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Sanjay Nigam

TitleProfessor
InstitutionUniversity of California San Diego
DepartmentPediatrics
Address9500 Gilman Drive #0693
La Jolla CA 92093
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    Collapse Overview 
    Collapse Overview
    A major focus of our lab is the biology and clinical importance of multi-specific "drug" transporters. We are particularly interested in the SLC22 family of transporters. Among these are the organic anion transporters (OATs) and organic cation transporters (OCTs) as well as other interesting SLC22 transporters--including a number discovered in the lab. These transporters are involved in the handling of many small molecule drugs, toxins and metabolites in the kidney and other organs (Nigam SK, Nature Reviews Drug Discovery 14, 29–44, 2015, see link under Dr. Nigam’s publications).

    We employ in vitro, ex vivo, in vivo (knockout) and computational methods. Current studies focus on SLC22 transporter endogenous function, systems pharmacology and developmental biology--with an eye toward understanding drug handling in infants and young children. A particular emphasis is OAT1, a major drug, toxin and metabolite transporter in the kidney and choroid plexus, which was first identified by our group as NKT. Among other functions, OAT1 appears to be a major transporter of uremic toxins that accumulate in chronic kidney disease (CKD).

    We also have a longstanding interest in kidney organogenesis and tissue engineering of the kidney.

    Research Focus Areas:

  • Developmental Biology

  • Glycobiology

  • Membrane Trafficking

  • Signal Transduction



  • Collapse Research 
    Collapse Research Activities and Funding
    The Role of OAT1 in Uremia
    NIH/NIDDK R01DK109392Jan 15, 2017 - Dec 31, 2020
    Role: Principal Investigator
    Pediatric and Developmental Pharmacology for Inflammatory and Infectious Diseases
    NIH/NICHD U54HD090259Sep 20, 2016 - Jun 30, 2021
    Role: Co-Investigator
    Structure Function Analysis of the Multi-specific Drug Transporter OCT1
    NIH/NIGMS R01GM104098Jun 1, 2013 - Feb 28, 2017
    Role: Principal Investigator
    Substrate Specificity of the Choroid Plexus and Kidney Transporter Oat1
    NIH/NIGMS R01GM098449Mar 5, 2012 - Feb 29, 2016
    Role: Principal Investigator
    Developmental and Translational Pharmacology of Pediatric Antimicrobial Therapy
    NIH/NICHD U54HD071600Sep 26, 2011 - Jun 30, 2016
    Role: Co-Investigator
    Systems Biology of Anionic Drug and Metabolite Handling
    NIH/NIGMS R01GM088824Aug 1, 2009 - Jun 30, 2013
    Role: Principal Investigator
    Combined Computational and Wet Lab Screening for Drugs Tested via OATs
    NIH/NINDS R01NS062156Apr 1, 2008 - Mar 31, 2012
    Role: Principal Investigator
    Mechanism of Fetal and Neonatal Handling of HIV Drugs
    NIH/NIDDK R01DK079784Mar 1, 2008 - Aug 31, 2012
    Role: Principal Investigator
    SNPs in Handling of Small Pox Antivirals and Other Drugs
    NIH/NIAID R01AI057695Jul 1, 2005 - Mar 31, 2011
    Role: Principal Investigator
    Identification of Genes Regulating Ureter Morphogenesis
    NIH/NIDDK R01DK065831Jan 1, 2004 - Nov 30, 2008
    Role: Principal Investigator
    Development &Maturation of Kidney Xenobiotic Transport
    NIH/NICHD R01HD040011Jul 1, 2001 - Jun 30, 2007
    Role: Principal Investigator
    Factor Mediating Ureteric Migration and Morphogenesis
    NIH/NIDDK R01DK057286Sep 30, 1999 - Nov 30, 2010
    Role: Principal Investigator
    KIDNEY DEVELOPMENT AND CYSTOGENESIS
    NIH P01DK054711Mar 1, 1999 - Feb 28, 2005
    Role: Co-Investigator
    KIDNEY ISCHEMIA AND TIGHT JUNCTION RECOVERY
    NIH/NIDDK R01DK053507Jan 1, 1998 - Dec 31, 2001
    Role: Principal Investigator
    CONFERENCE--MECHANISMS OF TISSUE INJURY AND REPAIR
    NIH/NIDDK R13DK053410Aug 1, 1997 - Sep 30, 1998
    Role: Principal Investigator
    ISCHEMIA &THE FOLDING OF MEMBRANE &SECRETED PROTEINS
    NIH/NIDDK R01DK051211Sep 15, 1996 - Jun 30, 2000
    Role: Principal Investigator
    DEVELOPMENT OF THE URINARY COLLECTING SYSTEM
    NIH/NIDDK R01DK049517Sep 1, 1994 - Nov 30, 2004
    Role: Principal Investigator
    KIDNEY EPITHELIAL MORPHOGENESIS AND CELLULAR SIGNALLING
    NIH/NIDDK R01DK044503Sep 30, 1992 - Sep 29, 1996
    Role: Principal Investigator
    GENETIC AND RECEPTOR MECHANISMS IN HYPERTENSION
    NIH/NHLBI P01HL035018Mar 1, 1986 - Dec 31, 2009
    Role: Co-Investigator
    CELLULAR TOPOGENESIS OF THE SODIUM GLUCOSE SYMPORTER
    NIH/NIADDK K11AM001467Sep 1, 1985 - Aug 31, 1990
    Role: Principal Investigator
    CHARACTERIZATION OF G PROTEINS IN THE ROUGH ENDOPLASMIC
    NIH/NIDDK K11DK001467Sep 1, 1985 - Aug 31, 1990
    Role: Principal Investigator

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    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Nigam SK, Bhatnagar V. The systems biology of uric acid transporters: the role of remote sensing and signaling. Curr Opin Nephrol Hypertens. 2018 Jul; 27(4):305-313. PMID: 29847376.
      View in: PubMed
    2. Momper JD, Nigam SK. Developmental regulation of kidney and liver SLC and ABC drug transporters and drug metabolizing enzymes: the role of remote organ communication. Expert Opin Drug Metab Toxicol. 2018 May 10. PMID: 29746174.
      View in: PubMed
    3. Fu Y, Breljak D, Onishi A, Batz F, Patel R, Huang W, Song P, Freeman B, Mayoux E, Koepsell H, Anzai N, Nigam SK, Sabolic I, Vallon V. The organic anion transporter OAT3 enhances the glucosuric effect of the SGLT2 inhibitor empagliflozin. Am J Physiol Renal Physiol. 2018 Feb 07. PMID: 29412698.
      View in: PubMed
    4. Nigam SK. The SLC22 Transporter Family: A Paradigm for the Impact of Drug Transporters on Metabolic Pathways, Signaling, and Disease. Annu Rev Pharmacol Toxicol. 2018 Jan 06; 58:663-687. PMID: 29309257.
      View in: PubMed
    5. Bush KT, Wu W, Lun C, Nigam SK. The drug transporter OAT3 (SLC22A8) and endogenous metabolite communication via the gut-liver-kidney axis. J Biol Chem. 2017 09 22; 292(38):15789-15803. PMID: 28765282.
      View in: PubMed
    6. Wu W, Bush KT, Nigam SK. Key Role for the Organic Anion Transporters, OAT1 and OAT3, in the in vivo Handling of Uremic Toxins and Solutes. Sci Rep. 2017 Jul 10; 7(1):4939. PMID: 28694431.
      View in: PubMed
    7. Liu HC, Goldenberg A, Chen Y, Lun C, Wu W, Bush KT, Balac N, Rodriguez P, Abagyan R, Nigam SK. Molecular Properties of Drugs Interacting with SLC22 Transporters OAT1, OAT3, OCT1, and OCT2: A Machine-Learning Approach. J Pharmacol Exp Ther. 2016 10; 359(1):215-29. PMID: 27488918.
      View in: PubMed
    8. Liu HC, Jamshidi N, Chen Y, Eraly SA, Cho SY, Bhatnagar V, Wu W, Bush KT, Abagyan R, Palsson BO, Nigam SK. An Organic Anion Transporter 1 (OAT1)-centered Metabolic Network. J Biol Chem. 2016 09 09; 291(37):19474-86. PMID: 27440044; PMCID: PMC5016685 [Available on 09/09/17].
    9. He X, Garza D, Nigam SK, Chang G. Multispecific Organic Cation Transporter 1 (OCT1) from Bos taurus Has High Affinity and Slow Binding Kinetics towards Prostaglandin E2. PLoS One. 2016; 11(4):e0152969. PMID: 27046168; PMCID: PMC4821612.
    10. Bhatnagar V, Richard EL, Wu W, Nievergelt CM, Lipkowitz MS, Jeff J, Maihofer AX, Nigam SK. Analysis of ABCG2 and other urate transporters in uric acid homeostasis in chronic kidney disease: potential role of remote sensing and signaling. Clin Kidney J. 2016 Jun; 9(3):444-53. PMID: 27274832; PMCID: PMC4886906.
    11. Martovetsky G, Bush KT, Nigam SK. Kidney versus Liver Specification of SLC and ABC Drug Transporters, Tight Junction Molecules, and Biomarkers. Drug Metab Dispos. 2016 07; 44(7):1050-60. PMID: 27044799.
      View in: PubMed
    12. Kumar N, Richter J, Cutts J, Bush KT, Trujillo C, Nigam SK, Gaasterland T, Brafman D, Willert K. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells. Elife. 2015 Nov 10; 4. PMID: 26554899; PMCID: PMC4631902.
    13. Zhu C, Nigam KB, Date RC, Bush KT, Springer SA, Saier MH, Wu W, Nigam SK. Evolutionary Analysis and Classification of OATs, OCTs, OCTNs, and Other SLC22 Transporters: Structure-Function Implications and Analysis of Sequence Motifs. PLoS One. 2015; 10(11):e0140569. PMID: 26536134.
      View in: PubMed
    14. Nigam SK, Wu W, Bush KT, Hoenig MP, Blantz RC, Bhatnagar V. Handling of Drugs, Metabolites, and Uremic Toxins by Kidney Proximal Tubule Drug Transporters. Clin J Am Soc Nephrol. 2015 Nov 06; 10(11):2039-49. PMID: 26490509; PMCID: PMC4633783.
    15. Wu W, Bush KT, Liu HC, Zhu C, Abagyan R, Nigam SK. Shared Ligands Between Organic Anion Transporters (OAT1 and OAT6) and Odorant Receptors. Drug Metab Dispos. 2015 Dec; 43(12):1855-63. PMID: 26358290; PMCID: PMC4658493 [Available on 12/01/16].
    16. Eraly SA, Liu HC, Jamshidi N, Nigam SK. Transcriptome-based reconstructions from the murine knockout suggest involvement of the urate transporter, URAT1 (slc22a12), in novel metabolic pathways. Biochem Biophys Rep. 2015 Sep 01; 3:51-61. PMID: 26251846.
      View in: PubMed
    17. Patel V, Carrion K, Hollands A, Hinton A, Gallegos T, Dyo J, Sasik R, Leire E, Hardiman G, Mohamed SA, Nigam S, King CC, Nizet V, Nigam V. The stretch responsive microRNA miR-148a-3p is a novel repressor of IKBKB, NF-?B signaling, and inflammatory gene expression in human aortic valve cells. FASEB J. 2015 May; 29(5):1859-68. PMID: 25630970; PMCID: PMC4771066.
    18. Nigam SK, Bush KT, Martovetsky G, Ahn SY, Liu HC, Richard E, Bhatnagar V, Wu W. The organic anion transporter (OAT) family: a systems biology perspective. Physiol Rev. 2015 Jan; 95(1):83-123. PMID: 25540139; PMCID: PMC4281586.
    19. Nigam SK. What do drug transporters really do? Nat Rev Drug Discov. 2015 Jan; 14(1):29-44. PMID: 25475361; PMCID: PMC4750486.
    20. Martovetsky G, Nigam SK. Cellular and developmental strategies aimed at kidney tissue engineering. Nephron Exp Nephrol. 2014; 126(2):101. PMID: 24854650.
      View in: PubMed
    21. Bush KT, Martovetsky G, Nigam SK. Relevance of ureteric bud development and branching to tissue engineering, regeneration and repair in acute and chronic kidney disease. Curr Opin Organ Transplant. 2014 Apr; 19(2):153-61. PMID: 24503495.
      View in: PubMed
    22. Nigam SK, Bush KT. Growth factor-heparan sulfate "switches" regulating stages of branching morphogenesis. Pediatr Nephrol. 2014 Apr; 29(4):727-35. PMID: 24488503.
      View in: PubMed
    23. Nigam SK. Concise review: can the intrinsic power of branching morphogenesis be used for engineering epithelial tissues and organs? Stem Cells Transl Med. 2013 Dec; 2(12):993-1000. PMID: 24191267; PMCID: PMC3841094.
    24. Sharma K, Karl B, Mathew AV, Gangoiti JA, Wassel CL, Saito R, Pu M, Sharma S, You YH, Wang L, Diamond-Stanic M, Lindenmeyer MT, Forsblom C, Wu W, Ix JH, Ideker T, Kopp JB, Nigam SK, Cohen CD, Groop PH, Barshop BA, Natarajan L, Nyhan WL, Naviaux RK. Metabolomics reveals signature of mitochondrial dysfunction in diabetic kidney disease. J Am Soc Nephrol. 2013 Nov; 24(11):1901-12. PMID: 23949796; PMCID: PMC3810086.
    25. Martovetsky G, Tee JB, Nigam SK. Hepatocyte nuclear factors 4a and 1a regulate kidney developmental expression of drug-metabolizing enzymes and drug transporters. Mol Pharmacol. 2013 Dec; 84(6):808-23. PMID: 24038112; PMCID: PMC3834141.
    26. Wu W, Jamshidi N, Eraly SA, Liu HC, Bush KT, Palsson BO, Nigam SK. Multispecific drug transporter Slc22a8 (Oat3) regulates multiple metabolic and signaling pathways. Drug Metab Dispos. 2013 Oct; 41(10):1825-34. PMID: 23920220; PMCID: PMC3781372.
    27. Tee JB, Choi Y, Dnyanmote A, Decambre M, Ito C, Bush KT, Nigam SK. GDNF-independent ureteric budding: role of PI3K-independent activation of AKT and FOSB/JUN/AP-1 signaling. Biol Open. 2013; 2(9):952-9. PMID: 24143282; PMCID: PMC3773342.
    28. Nigam SK, Bhatnagar V. How much do we know about drug handling by SLC and ABC drug transporters in children? Clin Pharmacol Ther. 2013 Jul; 94(1):27-9. PMID: 23778708.
      View in: PubMed
    29. Nagle MA, Wu W, Eraly SA, Nigam SK. Organic anion transport pathways in antiviral handling in choroid plexus in Oat1 (Slc22a6) and Oat3 (Slc22a8) deficient tissue. Neurosci Lett. 2013 Feb 08; 534:133-8. PMID: 23196129; PMCID: PMC3591490.
    30. Bush KT, Crawford BE, Garner OB, Nigam KB, Esko JD, Nigam SK. N-sulfation of heparan sulfate regulates early branching events in the developing mammary gland. J Biol Chem. 2012 Dec 07; 287(50):42064-70. PMID: 23060443; PMCID: PMC3516752.
    31. Nigam SK. The storytelling brain. Commentary on "On social attribution: implications of recent cognitive neuroscience research for race, law, and politics". Sci Eng Ethics. 2012 Sep; 18(3):567-71. PMID: 22843032.
      View in: PubMed
    32. Gallegos TF, Martovetsky G, Kouznetsova V, Bush KT, Nigam SK. Organic anion and cation SLC22 "drug" transporter (Oat1, Oat3, and Oct1) regulation during development and maturation of the kidney proximal tubule. PLoS One. 2012; 7(7):e40796. PMID: 22808265; PMCID: PMC3396597.
    33. Vallon V, Eraly SA, Rao SR, Gerasimova M, Rose M, Nagle M, Anzai N, Smith T, Sharma K, Nigam SK, Rieg T. A role for the organic anion transporter OAT3 in renal creatinine secretion in mice. Am J Physiol Renal Physiol. 2012 May 15; 302(10):F1293-9. PMID: 22338083.
      View in: PubMed
    34. Gallegos TF, Kouznetsova V, Kudlicka K, Sweeney DE, Bush KT, Willert K, Farquhar MG, Nigam SK. A protein kinase A and Wnt-dependent network regulating an intermediate stage in epithelial tubulogenesis during kidney development. Dev Biol. 2012 Apr 01; 364(1):11-21. PMID: 22290330; PMCID: PMC3310225.
    35. Zhang X, Bush KT, Nigam SK. In vitro culture of embryonic kidney rudiments and isolated ureteric buds. Methods Mol Biol. 2012; 886:13-21. PMID: 22639247.
      View in: PubMed
    36. Tsigelny IF, Kovalskyy D, Kouznetsova VL, Balinskyi O, Sharikov Y, Bhatnagar V, Nigam SK. Conformational changes of the multispecific transporter organic anion transporter 1 (OAT1/SLC22A6) suggests a molecular mechanism for initial stages of drug and metabolite transport. Cell Biochem Biophys. 2011 Nov; 61(2):251-9. PMID: 21499753.
      View in: PubMed
    37. Ahn SY, Jamshidi N, Mo ML, Wu W, Eraly SA, Dnyanmote A, Bush KT, Gallegos TF, Sweet DH, Palsson BØ, Nigam SK. Linkage of organic anion transporter-1 to metabolic pathways through integrated "omics"-driven network and functional analysis. J Biol Chem. 2011 Sep 09; 286(36):31522-31. PMID: 21757732; PMCID: PMC3173137.
    38. Torres AM, Dnyanmote AV, Bush KT, Wu W, Nigam SK. Deletion of multispecific organic anion transporter Oat1/Slc22a6 protects against mercury-induced kidney injury. J Biol Chem. 2011 Jul 29; 286(30):26391-5. PMID: 21652719; PMCID: PMC3143602.
    39. Shah MM, Sakurai H, Gallegos TF, Sweeney DE, Bush KT, Esko JD, Nigam SK. Growth factor-dependent branching of the ureteric bud is modulated by selective 6-O sulfation of heparan sulfate. Dev Biol. 2011 Aug 01; 356(1):19-27. PMID: 21600196; PMCID: PMC3130836.
    40. Garner OB, Bush KT, Nigam KB, Yamaguchi Y, Xu D, Esko JD, Nigam SK. Stage-dependent regulation of mammary ductal branching by heparan sulfate and HGF-cMet signaling. Dev Biol. 2011 Jul 15; 355(2):394-403. PMID: 21586278; PMCID: PMC3118867.
    41. Kouznetsova VL, Tsigelny IF, Nagle MA, Nigam SK. Elucidation of common pharmacophores from analysis of targeted metabolites transported by the multispecific drug transporter-Organic anion transporter1 (Oat1). Bioorg Med Chem. 2011 Jun 01; 19(11):3320-40. PMID: 21571536; PMCID: PMC3310230.
    42. Wikoff WR, Nagle MA, Kouznetsova VL, Tsigelny IF, Nigam SK. Untargeted metabolomics identifies enterobiome metabolites and putative uremic toxins as substrates of organic anion transporter 1 (Oat1). J Proteome Res. 2011 Jun 03; 10(6):2842-51. PMID: 21476605; PMCID: PMC3201759.
    43. Sweeney DE, Vallon V, Rieg T, Wu W, Gallegos TF, Nigam SK. Functional maturation of drug transporters in the developing, neonatal, and postnatal kidney. Mol Pharmacol. 2011 Jul; 80(1):147-54. PMID: 21493727; PMCID: PMC3127534.
    44. Wu W, Dnyanmote AV, Nigam SK. Remote communication through solute carriers and ATP binding cassette drug transporter pathways: an update on the remote sensing and signaling hypothesis. Mol Pharmacol. 2011 May; 79(5):795-805. PMID: 21325265; PMCID: PMC3082935.
    45. Sweeney DE, Gallegos TF, Dnyanmote AV, Zhang X, Nigam SK. Branching morphogenesis: from individual molecules to a systems biology approach: commentary on "Sema4C-Plexin B2 signalling modulates ureteric branching in developing kidney" by Perälä et al. Differentiation. 2011 Feb; 81(2):79-80. PMID: 21168258.
      View in: PubMed
    46. Nagle MA, Truong DM, Dnyanmote AV, Ahn SY, Eraly SA, Wu W, Nigam SK. Analysis of three-dimensional systems for developing and mature kidneys clarifies the role of OAT1 and OAT3 in antiviral handling. J Biol Chem. 2011 Jan 07; 286(1):243-51. PMID: 20921221; PMCID: PMC3012981.
    47. Tee JB, Choi Y, Shah MM, Dnyanmote A, Sweeney DE, Gallegos TF, Johkura K, Ito C, Bush KT, Nigam SK. Protein kinase A regulates GDNF/RET-dependent but not GDNF/Ret-independent ureteric bud outgrowth from the Wolffian duct. Dev Biol. 2010 Nov 15; 347(2):337-47. PMID: 20816800; PMCID: PMC2981800.
    48. Rosines E, Johkura K, Zhang X, Schmidt HJ, Decambre M, Bush KT, Nigam SK. Constructing kidney-like tissues from cells based on programs for organ development: toward a method of in vitro tissue engineering of the kidney. Tissue Eng Part A. 2010 Aug; 16(8):2441-55. PMID: 20214453; PMCID: PMC2947461.
    49. Crawford BE, Garner OB, Bishop JR, Zhang DY, Bush KT, Nigam SK, Esko JD. Loss of the heparan sulfate sulfotransferase, Ndst1, in mammary epithelial cells selectively blocks lobuloalveolar development in mice. PLoS One. 2010 May 18; 5(5):e10691. PMID: 20502530; PMCID: PMC2872662.
    50. Shah MM, Sakurai H, Sweeney DE, Gallegos TF, Bush KT, Esko JD, Nigam SK. Hs2st mediated kidney mesenchyme induction regulates early ureteric bud branching. Dev Biol. 2010 Mar 15; 339(2):354-65. PMID: 20059993; PMCID: PMC2834281.
    51. Choi Y, Tee JB, Gallegos TF, Shah MM, Oishi H, Sakurai H, Kitamura S, Wu W, Bush KT, Nigam SK. Neuropeptide Y functions as a facilitator of GDNF-induced budding of the Wolffian duct. Development. 2009 Dec; 136(24):4213-24. PMID: 19934016; PMCID: PMC2781055.
    52. Ahn SY, Eraly SA, Tsigelny I, Nigam SK. Interaction of organic cations with organic anion transporters. J Biol Chem. 2009 Nov 06; 284(45):31422-30. PMID: 19737926; PMCID: PMC2781538.
    53. Shah MM, Tee JB, Meyer T, Meyer-Schwesinger C, Choi Y, Sweeney DE, Gallegos TF, Johkura K, Rosines E, Kouznetsova V, Rose DW, Bush KT, Sakurai H, Nigam SK. The instructive role of metanephric mesenchyme in ureteric bud patterning, sculpting, and maturation and its potential ability to buffer ureteric bud branching defects. Am J Physiol Renal Physiol. 2009 Nov; 297(5):F1330-41. PMID: 19726549; PMCID: PMC2781331.
    54. Lancaster MA, Louie CM, Silhavy JL, Sintasath L, Decambre M, Nigam SK, Willert K, Gleeson JG. Impaired Wnt-beta-catenin signaling disrupts adult renal homeostasis and leads to cystic kidney ciliopathy. Nat Med. 2009 Sep; 15(9):1046-54. PMID: 19718039; PMCID: PMC2895985.
    55. Ahn SY, Nigam SK. Toward a systems level understanding of organic anion and other multispecific drug transporters: a remote sensing and signaling hypothesis. Mol Pharmacol. 2009 Sep; 76(3):481-90. PMID: 19515966; PMCID: PMC2730381.
    56. Wu W, Kitamura S, Truong DM, Rieg T, Vallon V, Sakurai H, Bush KT, Vera DR, Ross RS, Nigam SK. Beta1-integrin is required for kidney collecting duct morphogenesis and maintenance of renal function. Am J Physiol Renal Physiol. 2009 Jul; 297(1):F210-7. PMID: 19439520; PMCID: PMC2711709.
    57. Wu W, Baker ME, Eraly SA, Bush KT, Nigam SK. Analysis of a large cluster of SLC22 transporter genes, including novel USTs, reveals species-specific amplification of subsets of family members. Physiol Genomics. 2009 Jul 09; 38(2):116-24. PMID: 19417012; PMCID: PMC2712220.
    58. Tsigelny IF, Kouznetsova VL, Sweeney DE, Wu W, Bush KT, Nigam SK. Analysis of metagene portraits reveals distinct transitions during kidney organogenesis. Sci Signal. 2008 Dec 09; 1(49):ra16. PMID: 19066399; PMCID: PMC3016920.
    59. Nigam SK, Shah MM. How does the ureteric bud branch? J Am Soc Nephrol. 2009 Jul; 20(7):1465-9. PMID: 19056872.
      View in: PubMed
    60. Tsigelny IF, Greenberg J, Kouznetsova V, Nigam SK. Modeling of glycerol-3-phosphate transporter suggests a potential 'tilt' mechanism involved in its function. J Bioinform Comput Biol. 2008 Oct; 6(5):885-904. PMID: 18942157; PMCID: PMC2676871.
    61. Nigam SK, Wu W, Bush KT. Organogenesis forum lecture: In vitro kidney development, tissue engineering and systems biology. Organogenesis. 2008 Jul; 4(3):137-43. PMID: 19279725; PMCID: PMC2634587.
    62. Vallon V, Eraly SA, Wikoff WR, Rieg T, Kaler G, Truong DM, Ahn SY, Mahapatra NR, Mahata SK, Gangoiti JA, Wu W, Barshop BA, Siuzdak G, Nigam SK. Organic anion transporter 3 contributes to the regulation of blood pressure. J Am Soc Nephrol. 2008 Sep; 19(9):1732-40. PMID: 18508962; PMCID: PMC2518443.
    63. Eraly SA, Vallon V, Rieg T, Gangoiti JA, Wikoff WR, Siuzdak G, Barshop BA, Nigam SK. Multiple organic anion transporters contribute to net renal excretion of uric acid. Physiol Genomics. 2008 Apr 22; 33(2):180-92. PMID: 18270321; PMCID: PMC3016923.
    64. Sharikov Y, Walker RC, Greenberg J, Kouznetsova V, Nigam SK, Miller MA, Masliah E, Tsigelny IF. MAPAS: a tool for predicting membrane-contacting protein surfaces. Nat Methods. 2008 Feb; 5(2):119. PMID: 18235431.
      View in: PubMed
    65. Vallon V, Rieg T, Ahn SY, Wu W, Eraly SA, Nigam SK. Overlapping in vitro and in vivo specificities of the organic anion transporters OAT1 and OAT3 for loop and thiazide diuretics. Am J Physiol Renal Physiol. 2008 Apr; 294(4):F867-73. PMID: 18216144.
      View in: PubMed
    66. Truong DM, Kaler G, Khandelwal A, Swaan PW, Nigam SK. Multi-level analysis of organic anion transporters 1, 3, and 6 reveals major differences in structural determinants of antiviral discrimination. J Biol Chem. 2008 Mar 28; 283(13):8654-63. PMID: 18174163; PMCID: PMC2417182.
    67. Maeshima A, Sakurai H, Choi Y, Kitamura S, Vaughn DA, Tee JB, Nigam SK. Glial cell-derived neurotrophic factor independent ureteric bud outgrowth from the Wolffian duct. J Am Soc Nephrol. 2007 Dec; 18(12):3147-55. PMID: 18003772.
      View in: PubMed
    68. Rosines E, Schmidt HJ, Nigam SK. The effect of hyaluronic acid size and concentration on branching morphogenesis and tubule differentiation in developing kidney culture systems: potential applications to engineering of renal tissues. Biomaterials. 2007 Nov; 28(32):4806-17. PMID: 17706761; PMCID: PMC2700261.
    69. Nigam SK, Bush KT, Bhatnagar V. Drug and toxicant handling by the OAT organic anion transporters in the kidney and other tissues. Nat Clin Pract Nephrol. 2007 Aug; 3(8):443-8. PMID: 17653123.
      View in: PubMed
    70. Kaler G, Truong DM, Khandelwal A, Nagle M, Eraly SA, Swaan PW, Nigam SK. Structural variation governs substrate specificity for organic anion transporter (OAT) homologs. Potential remote sensing by OAT family members. J Biol Chem. 2007 Aug 17; 282(33):23841-53. PMID: 17553798; PMCID: PMC3812435.
    71. Monte JC, Sakurai H, Bush KT, Nigam SK. The developmental nephrome: systems biology in the developing kidney. Curr Opin Nephrol Hypertens. 2007 Jan; 16(1):3-9. PMID: 17143064.
      View in: PubMed
    72. Meyer TN, Schwesinger C, Sampogna RV, Vaughn DA, Stuart RO, Steer DL, Bush KT, Nigam SK. Rho kinase acts at separate steps in ureteric bud and metanephric mesenchyme morphogenesis during kidney development. Differentiation. 2006 Dec; 74(9-10):638-47. PMID: 17177859.
      View in: PubMed
    73. Kaler G, Truong DM, Sweeney DE, Logan DW, Nagle M, Wu W, Eraly SA, Nigam SK. Olfactory mucosa-expressed organic anion transporter, Oat6, manifests high affinity interactions with odorant organic anions. Biochem Biophys Res Commun. 2006 Dec 29; 351(4):872-6. PMID: 17094945; PMCID: PMC1810587.
    74. Bush KT, Vaughn DA, Li X, Rosenfeld MG, Rose DW, Mendoza SA, Nigam SK. Development and differentiation of the ureteric bud into the ureter in the absence of a kidney collecting system. Dev Biol. 2006 Oct 15; 298(2):571-84. PMID: 16934795.
      View in: PubMed
    75. Bhatnagar V, Xu G, Hamilton BA, Truong DM, Eraly SA, Wu W, Nigam SK. Analyses of 5' regulatory region polymorphisms in human SLC22A6 (OAT1) and SLC22A8 (OAT3). J Hum Genet. 2006; 51(6):575-80. PMID: 16648942.
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    76. Maeshima A, Vaughn DA, Choi Y, Nigam SK. Activin A is an endogenous inhibitor of ureteric bud outgrowth from the Wolffian duct. Dev Biol. 2006 Jul 15; 295(2):473-85. PMID: 16643884.
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    77. Eraly SA, Vallon V, Vaughn DA, Gangoiti JA, Richter K, Nagle M, Monte JC, Rieg T, Truong DM, Long JM, Barshop BA, Kaler G, Nigam SK. Decreased renal organic anion secretion and plasma accumulation of endogenous organic anions in OAT1 knock-out mice. J Biol Chem. 2006 Feb 24; 281(8):5072-83. PMID: 16354673.
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    78. Maeshima A, Sakurai H, Nigam SK. Adult kidney tubular cell population showing phenotypic plasticity, tubulogenic capacity, and integration capability into developing kidney. J Am Soc Nephrol. 2006 Jan; 17(1):188-98. PMID: 16338966.
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    79. Xu G, Bhatnagar V, Wen G, Hamilton BA, Eraly SA, Nigam SK. Analyses of coding region polymorphisms in apical and basolateral human organic anion transporter (OAT) genes [OAT1 (NKT), OAT2, OAT3, OAT4, URAT (RST)]. Kidney Int. 2005 Oct; 68(4):1491-9. PMID: 16164626.
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    80. Sakurai H, Bush KT, Nigam SK. Heregulin induces glial cell line-derived neurotrophic growth factor-independent, non-branching growth and differentiation of ureteric bud epithelia. J Biol Chem. 2005 Dec 23; 280(51):42181-7. PMID: 16183643.
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    81. Sampogna RV, Nigam SK. Implications of gene networks for understanding resilience and vulnerability in the kidney branching program. Physiology (Bethesda). 2004 Dec; 19:339-47. PMID: 15546851.
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    82. Meyer TN, Schwesinger C, Bush KT, Stuart RO, Rose DW, Shah MM, Vaughn DA, Steer DL, Nigam SK. Spatiotemporal regulation of morphogenetic molecules during in vitro branching of the isolated ureteric bud: toward a model of branching through budding in the developing kidney. Dev Biol. 2004 Nov 01; 275(1):44-67. PMID: 15464572.
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    83. Monte JC, Nagle MA, Eraly SA, Nigam SK. Identification of a novel murine organic anion transporter family member, OAT6, expressed in olfactory mucosa. Biochem Biophys Res Commun. 2004 Oct 15; 323(2):429-36. PMID: 15369770.
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    84. George SK, Meyer TN, Abdeen O, Bush KT, Nigam SK. Tunicamycin preserves intercellular junctions, cytoarchitecture, and cell-substratum interactions in ATP-depleted epithelial cells. Biochem Biophys Res Commun. 2004 Sep 10; 322(1):223-31. PMID: 15313195.
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    85. Steer DL, Shah MM, Bush KT, Stuart RO, Sampogna RV, Meyer TN, Schwesinger C, Bai X, Esko JD, Nigam SK. Regulation of ureteric bud branching morphogenesis by sulfated proteoglycans in the developing kidney. Dev Biol. 2004 Aug 15; 272(2):310-27. PMID: 15282150.
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    86. Eraly SA, Monte JC, Nigam SK. Novel slc22 transporter homologs in fly, worm, and human clarify the phylogeny of organic anion and cation transporters. Physiol Genomics. 2004 Jun 17; 18(1):12-24. PMID: 15054140.
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    87. Chen D, Roberts R, Pohl M, Nigam S, Kreidberg J, Wang Z, Heino J, Ivaska J, Coffa S, Harris RC, Pozzi A, Zent R. Differential expression of collagen- and laminin-binding integrins mediates ureteric bud and inner medullary collecting duct cell tubulogenesis. Am J Physiol Renal Physiol. 2004 Oct; 287(4):F602-11. PMID: 15187002.
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    88. Shah MM, Sampogna RV, Sakurai H, Bush KT, Nigam SK. Branching morphogenesis and kidney disease. Development. 2004 Apr; 131(7):1449-62. PMID: 15023929.
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    89. Eraly SA, Bush KT, Sampogna RV, Bhatnagar V, Nigam SK. The molecular pharmacology of organic anion transporters: from DNA to FDA? Mol Pharmacol. 2004 Mar; 65(3):479-87. PMID: 14978224.
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    90. Bush KT, Sakurai H, Steer DL, Leonard MO, Sampogna RV, Meyer TN, Schwesinger C, Qiao J, Nigam SK. TGF-beta superfamily members modulate growth, branching, shaping, and patterning of the ureteric bud. Dev Biol. 2004 Feb 15; 266(2):285-98. PMID: 14738877.
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    91. Steer DL, Nigam SK. Developmental approaches to kidney tissue engineering. Am J Physiol Renal Physiol. 2004 Jan; 286(1):F1-7. PMID: 14656756.
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    92. Tsigelny IF, Nigam SK. Complex dynamics of chaperone-protein interactions under cellular stress. Cell Biochem Biophys. 2004; 40(3):263-76. PMID: 15211027.
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    93. Sykes D, Sweet DH, Lowes S, Nigam SK, Pritchard JB, Miller DS. Organic anion transport in choroid plexus from wild-type and organic anion transporter 3 (Slc22a8)-null mice. Am J Physiol Renal Physiol. 2004 May; 286(5):F972-8. PMID: 15075193.
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    94. Stuart RO, Bush KT, Nigam SK. Changes in gene expression patterns in the ureteric bud and metanephric mesenchyme in models of kidney development. Kidney Int. 2003 Dec; 64(6):1997-2008. PMID: 14633122.
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    95. Nigam SK. From the ureteric bud to the penome. Kidney Int. 2003 Dec; 64(6):2320-2. PMID: 14633158.
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    96. Li X, Oghi KA, Zhang J, Krones A, Bush KT, Glass CK, Nigam SK, Aggarwal AK, Maas R, Rose DW, Rosenfeld MG. Eya protein phosphatase activity regulates Six1-Dach-Eya transcriptional effects in mammalian organogenesis. Nature. 2003 Nov 20; 426(6964):247-54. PMID: 14628042.
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    97. Eraly SA, Blantz RC, Bhatnagar V, Nigam SK. Novel aspects of renal organic anion transporters. Curr Opin Nephrol Hypertens. 2003 Sep; 12(5):551-8. PMID: 12920404.
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    98. Keller SH, Nigam SK. Biochemical processing of E-cadherin under cellular stress. Biochem Biophys Res Commun. 2003 Jul 25; 307(2):215-23. PMID: 12859942.
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    99. Li Z, Stuart RO, Eraly SA, Gittes G, Beier DR, Nigam SK. Debt91, a putative zinc finger protein differentially expressed during epithelial morphogenesis. Biochem Biophys Res Commun. 2003 Jul 04; 306(3):623-8. PMID: 12810064.
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    100. Eraly SA, Hamilton BA, Nigam SK. Organic anion and cation transporters occur in pairs of similar and similarly expressed genes. Biochem Biophys Res Commun. 2003 Jan 10; 300(2):333-42. PMID: 12504088.
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    101. Steer DL, Bush KT, Meyer TN, Schwesinger C, Nigam SK. A strategy for in vitro propagation of rat nephrons. Kidney Int. 2002 Dec; 62(6):1958-65. PMID: 12427120.
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    102. Eraly SA, Nigam SK. Novel human cDNAs homologous to Drosophila Orct and mammalian carnitine transporters. Biochem Biophys Res Commun. 2002 Oct 11; 297(5):1159-66. PMID: 12372408.
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    103. Sweet DH, Miller DS, Pritchard JB, Fujiwara Y, Beier DR, Nigam SK. Impaired organic anion transport in kidney and choroid plexus of organic anion transporter 3 (Oat3 (Slc22a8)) knockout mice. J Biol Chem. 2002 Jul 26; 277(30):26934-43. PMID: 12011098.
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    104. Pohl M, Bhatnagar V, Mendoza SA, Nigam SK. Toward an etiological classification of developmental disorders of the kidney and upper urinary tract. Kidney Int. 2002 Jan; 61(1):10-9. PMID: 11786080.
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