Loading...
Sign in to edit your profile (add interests, mentoring, photo, etc.)

    Zoran Radic

    TitleAssociate Adjunct Professor
    SchoolUniversity of California, San Diego
    DepartmentSchool of Pharmacy and Pharmaceutical Science
    Address9500 Gilman Drive #0650
    CA La Jolla 92093
    Phone858-534-6841
    vCardDownload vCard

      Collapse Biography 
      Collapse Awards and Honors
      Croatian Society of Natural Sciences2016  - 2017Spiridion Brusina Medal
      New York Academy of Science2016  - 2017Professional Member (by invitation)

      Collapse Overview 
      Collapse Overview
      Dr. Radic is a graduate of the University of Zagreb in Croatia where he started his research in acetylcholinesterase (AChE) reaction kinetics under mentorship of late Dr. Elsa Reiner, one of founding contributors in the field of cholinesterases. His interests in the AChE molecular structure brought him to the laboratory of Dr. Palmer Taylor at UCSD where he provided key contributions to functional mapping of the AChE molecule using site directed mutagenesis and defined structural domains and amino acid residues critical for catalytic activity and ligand interaction. He was the first to propose common, simplified kinetic scheme and equation to describe acetylcholine (ACh) turnover by both AChE and its structurally and functionally close relative butyrylcholinesterase (BChE) that included both substrate inhibition of AChE and substrate activation of BChE, two prominent forms of deviation from Michaelis-Menten kinetcs. Later this scheme and mechanism proved useful for the description of oxime reactivation kinetics of nerve agent organophosphate (OP) and OP pesticide conjugated AChE and BChE including positive allosteric modulation in reactivation of OP-BChE and negative allosteric modulation of OP-AChE conjugates. Dr. Radic developed intrinsic tryptophane fluorescence based assays as a highly valuable tool for in vitro monitoring of time resolved and equilibrium interactions of cholinergic ligands with native nicotinic acetylcholine receptor ligand binding domain surrogates, ACh binding proteins (AChBPs), as well as with AChE and BChE. This assay proved essential in functional characterization of one of tightest known, high affinity binding small molecule femtomolar AChE inhibitors developed in collaboration with Dr. Barry Sharpless of The Scripps Research Institute in La Jolla (TSRI). Dr. Radic leads, as a PI, NIH funded international collaborative project with researchers from TSRI (Dr. Barry K. Sharpless and Dr. Valery Fokin) and Institute for Medical Research and Occupational Health in Zagreb, Croatia (Dr. Zrinka Kovarik) towards development of novel, improved oxime reactivators of OP-AChE and OP-BChE conjugates, both in vitro and in vivo.


      Collapse Research 
      Collapse Research Activities and Funding
      Overlay independent X-ray data analysis for enhanced oxime OP-ChE reactivation
      NIH/NINDS R21NS098998Sep 30, 2016 - Aug 31, 2018
      Role: Principal Investigator
      Accelerated AChE Reactivator Design by Mechanistic Neutron Scattering Studies
      NIH/NINDS U01NS083451Jul 15, 2014 - Jun 30, 2019
      Role: Principal Investigator
      BChE reactivators for nerve agent and pesticide OP detoxification in human tissue
      NIH/NINDS R21NS084904Sep 30, 2013 - Aug 31, 2015
      Role: Principal Investigator
      Optimization of nonpyridinium oximes for BChE hydrolysis of OPs in plasma
      NIH/NINDS R21NS072086Sep 30, 2010 - Aug 31, 2013
      Role: Principal Investigator

      Collapse ORNG Applications 
      Collapse Featured Videos
      Collapse Featured Presentations

      Collapse Bibliographic 
      Collapse Publications
      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
      List All   |   Timeline
      1. Rosenberg YJ, Mao L, Jiang X, Lees J, Zhang L, Radic Z, Taylor P. Post-exposure treatment with the oxime RS194B rapidly reverses early and advanced symptoms in macaques exposed to sarin vapor. Chem Biol Interact. 2017 Aug 25; 274:50-57. PMID: 28693885.
        View in: PubMed
      2. Kovalevsky A, Blumenthal DK, Cheng X, Taylor P, Radic Z. Limitations in current acetylcholinesterase structure-based design of oxime antidotes for organophosphate poisoning. Ann N Y Acad Sci. 2016 Aug; 1378(1):41-49. PMID: 27371941.
        View in: PubMed
      3. de Lima WE, Francisco A, da Cunha EF, Radic Z, Taylor P, França TC, Ramalho TC. Mechanistic studies of new oximes reactivators of human butyryl cholinesterase inhibited by cyclosarin and sarin. J Biomol Struct Dyn. 2017 May; 35(6):1272-1282. PMID: 27125569.
        View in: PubMed
      4. Macek Hrvat N, Žunec S, Taylor P, Radic Z, Kovarik Z. HI-6 assisted catalytic scavenging of VX by acetylcholinesterase choline binding site mutants. Chem Biol Interact. 2016 Nov 25; 259(Pt B):148-153. PMID: 27083141.
        View in: PubMed
      5. Mangas I, Radic Z, Taylor P, Ghassemian M, Candela H, Vilanova E, Estévez J. Butyrylcholinesterase identification in a phenylvalerate esterase-enriched fraction sensitive to low mipafox concentrations in chicken brain. Arch Toxicol. 2017 Feb; 91(2):909-919. PMID: 26838044.
        View in: PubMed
      6. Bourne Y, Sulzenbacher G, Radic Z, Aráoz R, Reynaud M, Benoit E, Zakarian A, Servent D, Molgó J, Taylor P, Marchot P. Marine Macrocyclic Imines, Pinnatoxins A and G: Structural Determinants and Functional Properties to Distinguish Neuronal a7 from Muscle a1(2)ß?d nAChRs. Structure. 2015 Jun 02; 23(6):1106-15. PMID: 26004441; PMCID: PMC4461042.
      7. Kovarik Z, Macek Hrvat N, Katalinic M, Sit RK, Paradyse A, Žunec S, Musilek K, Fokin VV, Taylor P, Radic Z. Catalytic Soman Scavenging by the Y337A/F338A Acetylcholinesterase Mutant Assisted with Novel Site-Directed Aldoximes. Chem Res Toxicol. 2015 May 18; 28(5):1036-44. PMID: 25835984; PMCID: PMC4791098.
      8. Schmidt HR, Radic Z, Taylor P, Fradinger EA. Quaternary and tertiary aldoxime antidotes for organophosphate exposure in a zebrafish model system. Toxicol Appl Pharmacol. 2015 Apr 15; 284(2):197-203. PMID: 25701203.
        View in: PubMed
      9. Kaczanowska K, Harel M, Radic Z, Changeux JP, Finn MG, Taylor P. Structural basis for cooperative interactions of substituted 2-aminopyrimidines with the acetylcholine binding protein. Proc Natl Acad Sci U S A. 2014 Jul 22; 111(29):10749-54. PMID: 25006260; PMCID: PMC4115551.
      10. Sit RK, Fokin VV, Amitai G, Sharpless KB, Taylor P, Radic Z. Imidazole aldoximes effective in assisting butyrylcholinesterase catalysis of organophosphate detoxification. J Med Chem. 2014 Feb 27; 57(4):1378-89. PMID: 24571195; PMCID: PMC4167068.
      11. Geng L, Gao Y, Chen X, Hou S, Zhan CG, Radic Z, Parks RJ, Russell SJ, Pham L, Brimijoin S. Gene transfer of mutant mouse cholinesterase provides high lifetime expression and reduced cocaine responses with no evident toxicity. PLoS One. 2013; 8(6):e67446. PMID: 23840704; PMCID: PMC3696080.
      12. Morris GM, Green LG, Radic Z, Taylor P, Sharpless KB, Olson AJ, Grynszpan F. Automated docking with protein flexibility in the design of femtomolar "click chemistry" inhibitors of acetylcholinesterase. J Chem Inf Model. 2013 Apr 22; 53(4):898-906. PMID: 23451944; PMCID: PMC3698963.
      13. Radic Z, Dale T, Kovarik Z, Berend S, Garcia E, Zhang L, Amitai G, Green C, Radic B, Duggan BM, Ajami D, Rebek J, Taylor P. Catalytic detoxification of nerve agent and pesticide organophosphates by butyrylcholinesterase assisted with non-pyridinium oximes. Biochem J. 2013 Feb 15; 450(1):231-42. PMID: 23216060; PMCID: PMC4772673.
      14. Kovarik Z, Macek N, Sit RK, Radic Z, Fokin VV, Barry Sharpless K, Taylor P. Centrally acting oximes in reactivation of tabun-phosphoramidated AChE. Chem Biol Interact. 2013 Mar 25; 203(1):77-80. PMID: 22960624; PMCID: PMC3530643.
      15. Radic Z, Sit RK, Garcia E, Zhang L, Berend S, Kovarik Z, Amitai G, Fokin VV, Barry Sharpless K, Taylor P. Mechanism of interaction of novel uncharged, centrally active reactivators with OP-hAChE conjugates. Chem Biol Interact. 2013 Mar 25; 203(1):67-71. PMID: 22975155; PMCID: PMC3561488.
      16. Radic Z, Sit RK, Kovarik Z, Berend S, Garcia E, Zhang L, Amitai G, Green C, Radic B, Fokin VV, Sharpless KB, Taylor P. Refinement of structural leads for centrally acting oxime reactivators of phosphylated cholinesterases. J Biol Chem. 2012 Apr 06; 287(15):11798-809. PMID: 22343626; PMCID: PMC3320928.
      17. Cochran R, Kalisiak J, Küçükkilinç T, Radic Z, Garcia E, Zhang L, Ho KY, Amitai G, Kovarik Z, Fokin VV, Sharpless KB, Taylor P. Oxime-assisted acetylcholinesterase catalytic scavengers of organophosphates that resist aging. J Biol Chem. 2011 Aug 26; 286(34):29718-24. PMID: 21730071; PMCID: PMC3191013.
      18. Valle AM, Radic Z, Rana BK, Mahboubi V, Wessel J, Shih PA, Rao F, O'Connor DT, Taylor P. Naturally occurring variations in the human cholinesterase genes: heritability and association with cardiovascular and metabolic traits. J Pharmacol Exp Ther. 2011 Jul; 338(1):125-33. PMID: 21493754; PMCID: PMC3126649.
      19. Sit RK, Radic Z, Gerardi V, Zhang L, Garcia E, Katalinic M, Amitai G, Kovarik Z, Fokin VV, Sharpless KB, Taylor P. New structural scaffolds for centrally acting oxime reactivators of phosphylated cholinesterases. J Biol Chem. 2011 Jun 03; 286(22):19422-30. PMID: 21464125; PMCID: PMC3103321.
      20. Bourne Y, Radic Z, Taylor P, Marchot P. Conformational remodeling of femtomolar inhibitor-acetylcholinesterase complexes in the crystalline state. J Am Chem Soc. 2010 Dec 29; 132(51):18292-300. PMID: 21090615; PMCID: PMC3016896.
      21. Radic Z, Kalisiak J, Fokin VV, Sharpless KB, Taylor P. Interaction kinetics of oximes with native, phosphylated and aged human acetylcholinesterase. Chem Biol Interact. 2010 Sep 06; 187(1-3):163-6. PMID: 20412789; PMCID: PMC2912944.
      22. Küçükkilinç T, Cochran R, Kalisiak J, Garcia E, Valle A, Amitai G, Radic Z, Taylor P. Investigating the structural influence of surface mutations on acetylcholinesterase inhibition by organophosphorus compounds and oxime reactivation. Chem Biol Interact. 2010 Sep 06; 187(1-3):238-40. PMID: 20382137; PMCID: PMC2933076.
      23. Bourne Y, Radic Z, Aráoz R, Talley TT, Benoit E, Servent D, Taylor P, Molgó J, Marchot P. Structural determinants in phycotoxins and AChBP conferring high affinity binding and nicotinic AChR antagonism. Proc Natl Acad Sci U S A. 2010 Mar 30; 107(13):6076-81. PMID: 20224036; PMCID: PMC2851920.
      24. Talley TT, Harel M, Hibbs RE, Radic Z, Tomizawa M, Casida JE, Taylor P. Atomic interactions of neonicotinoid agonists with AChBP: molecular recognition of the distinctive electronegative pharmacophore. Proc Natl Acad Sci U S A. 2008 May 27; 105(21):7606-11. PMID: 18477694; PMCID: PMC2396707.
      25. Radic Z, Manetsch R, Fournier D, Sharpless KB, Taylor P. Probing gorge dimensions of cholinesterases by freeze-frame click chemistry. Chem Biol Interact. 2008 Sep 25; 175(1-3):161-5. PMID: 18555981; PMCID: PMC2585062.
      26. Cheng YH, Cheng XL, Radic Z, McCammon JA. Acetylcholinesterase: mechanisms of covalent inhibition of H447I mutant determined by computational analyses. Chem Biol Interact. 2008 Sep 25; 175(1-3):196-9. PMID: 18657802; PMCID: PMC2576475.
      27. Valle AM, Radic Z, Rana BK, Whitfield JB, O'Connor DT, Martin NG, Taylor P. The cholinesterases: analysis by pharmacogenomics in man. Chem Biol Interact. 2008 Sep 25; 175(1-3):343-5. PMID: 18541229; PMCID: PMC2585411.
      28. Reiner E, Radic Z, Simeon-Rudolf V. Mechanisms of organophosphate toxicity and detoxication with emphasis on studies in Croatia. Arh Hig Rada Toksikol. 2007 Sep; 58(3):329-38. PMID: 17913688.
        View in: PubMed
      29. Taylor P, Reiner E, Kovarik Z, Radic Z. Application of recombinant DNA methods for production of cholinesterases as organophosphate antidotes and detectors. Arh Hig Rada Toksikol. 2007 Sep; 58(3):339-45. PMID: 17913689.
        View in: PubMed
      30. Cheng Y, Cheng X, Radic Z, McCammon JA. Acetylcholinesterase: mechanisms of covalent inhibition of wild-type and H447I mutant determined by computational analyses. J Am Chem Soc. 2007 May 23; 129(20):6562-70. PMID: 17461584.
        View in: PubMed
      31. Kang TS, Radic Z, Talley TT, Jois SD, Taylor P, Kini RM. Protein folding determinants: structural features determining alternative disulfide pairing in alpha- and chi/lambda-conotoxins. Biochemistry. 2007 Mar 20; 46(11):3338-55. PMID: 17315952; PMCID: PMC4778399.
      32. Cheng Y, Suen JK, Radic Z, Bond SD, Holst MJ, McCammon JA. Continuum simulations of acetylcholine diffusion with reaction-determined boundaries in neuromuscular junction models. Biophys Chem. 2007 May; 127(3):129-39. PMID: 17307283; PMCID: PMC2040065.
      33. Taylor P, Kovarik Z, Reiner E, Radic Z. Acetylcholinesterase: converting a vulnerable target to a template for antidotes and detection of inhibitor exposure. Toxicology. 2007 Apr 20; 233(1-3):70-8. PMID: 17196318; PMCID: PMC3279330.
      34. Hibbs RE, Radic Z, Taylor P, Johnson DA. Influence of agonists and antagonists on the segmental motion of residues near the agonist binding pocket of the acetylcholine-binding protein. J Biol Chem. 2006 Dec 22; 281(51):39708-18. PMID: 17068341.
        View in: PubMed
      35. Kovarik Z, Radic Z, Berman HA, Taylor P. Mutation of acetylcholinesterase to enhance oxime-assisted catalytic turnover of methylphosphonates. Toxicology. 2007 Apr 20; 233(1-3):79-84. PMID: 17046138.
        View in: PubMed
      36. Bourne Y, Radic Z, Sulzenbacher G, Kim E, Taylor P, Marchot P. Substrate and product trafficking through the active center gorge of acetylcholinesterase analyzed by crystallography and equilibrium binding. J Biol Chem. 2006 Sep 29; 281(39):29256-67. PMID: 16837465.
        View in: PubMed
      37. Kovarik Z, Ciban N, Radic Z, Simeon-Rudolf V, Taylor P. Active site mutant acetylcholinesterase interactions with 2-PAM, HI-6, and DDVP. Biochem Biophys Res Commun. 2006 Apr 14; 342(3):973-8. PMID: 16598855.
        View in: PubMed
      38. Bui JM, Radic Z, Taylor P, McCammon JA. Conformational transitions in protein-protein association: binding of fasciculin-2 to acetylcholinesterase. Biophys J. 2006 May 01; 90(9):3280-7. PMID: 16473897; PMCID: PMC1432132.
      39. De Jaco A, Comoletti D, Kovarik Z, Gaietta G, Radic Z, Lockridge O, Ellisman MH, Taylor P. A mutation linked with autism reveals a common mechanism of endoplasmic reticulum retention for the alpha,beta-hydrolase fold protein family. J Biol Chem. 2006 Apr 07; 281(14):9667-76. PMID: 16434405.
        View in: PubMed
      40. Kovarik Z, Radic Z, Simeon-Rudolf V, Reiner E, Taylor P. Acetylcholinesterase mutants: oxime-assisted catalytic scavengers of organophosphonates. Chem Biol Interact. 2005 Dec 15; 157-158:388-90. PMID: 16429526.
        View in: PubMed
      41. Luo C, Dawson M, Chambers C, Chilukuri N, Radic Z, Taylor P, Doctor BP, Saxena A. Species-related differences in the oxime-induced reactivation of organophosphate-inhibited acetylcholinesterases. Chem Biol Interact. 2005 Dec 15; 157-158:393-4. PMID: 16429528.
        View in: PubMed
      42. Pezzementi L, Shi J, Johnson DA, Radic Z, Boyd A, Taylor P. Ligand-induced conformational changes in residues flanking the active site gorge of acetylcholinesterase. Chem Biol Interact. 2005 Dec 15; 157-158:413-4. PMID: 16429560.
        View in: PubMed
      43. Radic Z, Manetsch R, Krasinski A, Raushel J, Yamauchi J, Garcia C, Kolb H, Sharpless KB, Taylor P. Molecular basis of interactions of cholinesterases with tight binding inhibitors. Chem Biol Interact. 2005 Dec 15; 157-158:133-41. PMID: 16289416.
        View in: PubMed
      44. Bourne Y, Radic Z, Kolb HC, Sharpless KB, Taylor P, Marchot P. Structural insights into conformational flexibility at the peripheral site and within the active center gorge of AChE. Chem Biol Interact. 2005 Dec 15; 157-158:159-65. PMID: 16259971.
        View in: PubMed
      45. Krasinski A, Radic Z, Manetsch R, Raushel J, Taylor P, Sharpless KB, Kolb HC. In situ selection of lead compounds by click chemistry: target-guided optimization of acetylcholinesterase inhibitors. J Am Chem Soc. 2005 May 11; 127(18):6686-92. PMID: 15869290.
        View in: PubMed
      46. Zhang D, Suen J, Zhang Y, Song Y, Radic Z, Taylor P, Holst MJ, Bajaj C, Baker NA, McCammon JA. Tetrameric mouse acetylcholinesterase: continuum diffusion rate calculations by solving the steady-state Smoluchowski equation using finite element methods. Biophys J. 2005 Mar; 88(3):1659-65. PMID: 15626705; PMCID: PMC1305222.
      47. Manetsch R, Krasinski A, Radic Z, Raushel J, Taylor P, Sharpless KB, Kolb HC. In situ click chemistry: enzyme inhibitors made to their own specifications. J Am Chem Soc. 2004 Oct 13; 126(40):12809-18. PMID: 15469276.
        View in: PubMed
      48. Taylor P, Hansen SB, Talley TT, Hibbs RE, Radic Z. Contemporary paradigms for cholinergic ligand design guided by biological structure. Bioorg Med Chem Lett. 2004 Apr 19; 14(8):1875-7. PMID: 15050619; PMCID: PMC3191913.
      49. Boyd AE, Dunlop CS, Wong L, Radic Z, Taylor P, Johnson DA. Nanosecond dynamics of acetylcholinesterase near the active center gorge. J Biol Chem. 2004 Jun 18; 279(25):26612-8. PMID: 15078872.
        View in: PubMed
      50. Hansen SB, Talley TT, Radic Z, Taylor P. Structural and ligand recognition characteristics of an acetylcholine-binding protein from Aplysia californica. J Biol Chem. 2004 Jun 04; 279(23):24197-202. PMID: 15069068; PMCID: PMC4762456.
      51. Kovarik Z, Radic Z, Berman HA, Simeon-Rudolf V, Reiner E, Taylor P. Mutant cholinesterases possessing enhanced capacity for reactivation of their phosphonylated conjugates. Biochemistry. 2004 Mar 23; 43(11):3222-9. PMID: 15023072.
        View in: PubMed
      52. Bourne Y, Kolb HC, Radic Z, Sharpless KB, Taylor P, Marchot P. Freeze-frame inhibitor captures acetylcholinesterase in a unique conformation. Proc Natl Acad Sci U S A. 2004 Feb 10; 101(6):1449-54. PMID: 14757816; PMCID: PMC341740.
      53. Saxena A, Fedorko JM, Vinayaka CR, Medhekar R, Radic Z, Taylor P, Lockridge O, Doctor BP. Aromatic amino-acid residues at the active and peripheral anionic sites control the binding of E2020 (Aricept) to cholinesterases. Eur J Biochem. 2003 Nov; 270(22):4447-58. PMID: 14622273.
        View in: PubMed
      54. Luo C, Leader H, Radic Z, Maxwell DM, Taylor P, Doctor BP, Saxena A. Two possible orientations of the HI-6 molecule in the reactivation of organophosphate-inhibited acetylcholinesterase. Biochem Pharmacol. 2003 Aug 01; 66(3):387-92. PMID: 12907237.
        View in: PubMed
      55. Kovarik Z, Radic Z, Berman HA, Simeon-Rudolf V, Reiner E, Taylor P. Acetylcholinesterase active centre and gorge conformations analysed by combinatorial mutations and enantiomeric phosphonates. Biochem J. 2003 Jul 01; 373(Pt 1):33-40. PMID: 12665427; PMCID: PMC1223469.
      56. Bourne Y, Taylor P, Radic Z, Marchot P. Structural insights into ligand interactions at the acetylcholinesterase peripheral anionic site. EMBO J. 2003 Jan 02; 22(1):1-12. PMID: 12505979; PMCID: PMC140045.
      57. Lewis WG, Green LG, Grynszpan F, Radic Z, Carlier PR, Taylor P, Finn MG, Sharpless KB. Click chemistry in situ: acetylcholinesterase as a reaction vessel for the selective assembly of a femtomolar inhibitor from an array of building blocks. Angew Chem Int Ed Engl. 2002 Mar 15; 41(6):1053-7. PMID: 12491310.
        View in: PubMed
      58. Radic Z, Taylor P. Peripheral site ligands accelerate inhibition of acetylcholinesterase by neutral organophosphates. J Appl Toxicol. 2001 Dec; 21 Suppl 1:S13-4. PMID: 11920914.
        View in: PubMed
      59. Shi J, Boyd AE, Radic Z, Taylor P. Reversibly bound and covalently attached ligands induce conformational changes in the omega loop, Cys69-Cys96, of mouse acetylcholinesterase. J Biol Chem. 2001 Nov 09; 276(45):42196-204. PMID: 11517229.
        View in: PubMed
      60. Radic Z, Taylor P. Interaction kinetics of reversible inhibitors and substrates with acetylcholinesterase and its fasciculin 2 complex. J Biol Chem. 2001 Feb 16; 276(7):4622-33. PMID: 11036076.
        View in: PubMed
      61. Wong L, Radic Z, Brüggemann RJ, Hosea N, Berman HA, Taylor P. Mechanism of oxime reactivation of acetylcholinesterase analyzed by chirality and mutagenesis. Biochemistry. 2000 May 16; 39(19):5750-7. PMID: 10801325.
        View in: PubMed
      62. Radic Z, Taylor P. The influence of peripheral site ligands on the reaction of symmetric and chiral organophosphates with wildtype and mutant acetylcholinesterases. Chem Biol Interact. 1999 May 14; 119-120:111-7. PMID: 10421444.
        View in: PubMed
      63. Taylor P, Wong L, Radic Z, Tsigelny I, Brüggemann R, Hosea NA, Berman HA. Analysis of cholinesterase inactivation and reactivation by systematic structural modification and enantiomeric selectivity. Chem Biol Interact. 1999 May 14; 119-120:3-15. PMID: 10421434.
        View in: PubMed
      64. Tara S, Elcock AH, Kirchhoff PD, Briggs JM, Radic Z, Taylor P, McCammon JA. Rapid binding of a cationic active site inhibitor to wild type and mutant mouse acetylcholinesterase: Brownian dynamics simulation including diffusion in the active site gorge. Biopolymers. 1998 Dec; 46(7):465-74. PMID: 9838872.
        View in: PubMed
      65. Radic Z, Kirchhoff PD, Quinn DM, McCammon JA, Taylor P. Electrostatic influence on the kinetics of ligand binding to acetylcholinesterase. Distinctions between active center ligands and fasciculin. J Biol Chem. 1997 Sep 12; 272(37):23265-77. PMID: 9287336.
        View in: PubMed
      66. Marchot P, Prowse CN, Kanter J, Camp S, Ackermann EJ, Radic Z, Bougis PE, Taylor P. Expression and activity of mutants of fasciculin, a peptidic acetylcholinesterase inhibitor from mamba venom. J Biol Chem. 1997 Feb 07; 272(6):3502-10. PMID: 9013597.
        View in: PubMed
      67. Taylor P, Hosea NA, Tsigelny I, Radic Z, Berman HA. Determining ligand orientation and transphosphonylation mechanisms on acetylcholinesterase by Rp, Sp enantiomer selectivity and site-specific mutagenesis. Enantiomer. 1997; 2(3-4):249-60. PMID: 9676269.
        View in: PubMed
      68. Hosea NA, Radic Z, Tsigelny I, Berman HA, Quinn DM, Taylor P. Aspartate 74 as a primary determinant in acetylcholinesterase governing specificity to cationic organophosphonates. Biochemistry. 1996 Aug 20; 35(33):10995-1004. PMID: 8718893.
        View in: PubMed
      69. Taylor P, Radic Z, Hosea NA, Camp S, Marchot P, Berman HA. Structural bases for the specificity of cholinesterase catalysis and inhibition. Toxicol Lett. 1995 Dec; 82-83:453-8. PMID: 8597093.
        View in: PubMed
      70. Radic Z, Quinn DM, Vellom DC, Camp S, Taylor P. Allosteric control of acetylcholinesterase catalysis by fasciculin. J Biol Chem. 1995 Sep 01; 270(35):20391-9. PMID: 7657613.
        View in: PubMed
      71. van den Born HK, Radic Z, Marchot P, Taylor P, Tsigelny I. Theoretical analysis of the structure of the peptide fasciculin and its docking to acetylcholinesterase. Protein Sci. 1995 Apr; 4(4):703-15. PMID: 7613468; PMCID: PMC2143104.
      72. Taylor P, Radic Z, Kreienkamp HJ, Maeda R, Luo Z, Fuentes ME, Vellom D, Pickering N. Expression and ligand specificity of acetylcholinesterase and the nicotinic receptor: a tale of two cholinergic sites. Biochem Soc Trans. 1994 Aug; 22(3):740-5. PMID: 7821676.
        View in: PubMed
      73. Radic Z, Duran R, Vellom DC, Li Y, Cervenansky C, Taylor P. Site of fasciculin interaction with acetylcholinesterase. J Biol Chem. 1994 Apr 15; 269(15):11233-9. PMID: 8157652.
        View in: PubMed
      74. Taylor P, Radic Z. The cholinesterases: from genes to proteins. Annu Rev Pharmacol Toxicol. 1994; 34:281-320. PMID: 8042853.
        View in: PubMed
      75. Radic Z, Pickering NA, Vellom DC, Camp S, Taylor P. Three distinct domains in the cholinesterase molecule confer selectivity for acetyl- and butyrylcholinesterase inhibitors. Biochemistry. 1993 Nov 16; 32(45):12074-84. PMID: 8218285.
        View in: PubMed
      76. Taylor P, Li Y, Camp S, Rachinsky TL, Ekström T, Getman D, Fuentes ME, Vellom DC, Radic Z. Structure and regulation of expression of the acetylcholinesterase gene. Chem Biol Interact. 1993 Jun; 87(1-3):199-207. PMID: 8343976.
        View in: PubMed
      77. Vellom DC, Radic Z, Li Y, Pickering NA, Camp S, Taylor P. Amino acid residues controlling acetylcholinesterase and butyrylcholinesterase specificity. Biochemistry. 1993 Jan 12; 32(1):12-7. PMID: 8418833.
        View in: PubMed
      78. Radic Z, Gibney G, Kawamoto S, MacPhee-Quigley K, Bongiorno C, Taylor P. Expression of recombinant acetylcholinesterase in a baculovirus system: kinetic properties of glutamate 199 mutants. Biochemistry. 1992 Oct 13; 31(40):9760-7. PMID: 1356436.
        View in: PubMed
      79. Radic Z, Reiner E, Taylor P. Role of the peripheral anionic site on acetylcholinesterase: inhibition by substrates and coumarin derivatives. Mol Pharmacol. 1991 Jan; 39(1):98-104. PMID: 1987454.
        View in: PubMed
      80. Abramson SN, Radic Z, Manker D, Faulkner DJ, Taylor P. Onchidal: a naturally occurring irreversible inhibitor of acetylcholinesterase with a novel mechanism of action. Mol Pharmacol. 1989 Sep; 36(3):349-54. PMID: 2779521.
        View in: PubMed