Loading...
Sign in to edit your profile (add interests, mentoring, photo, etc.)

    Richard Daneman

    TitleAssistant Professor
    SchoolUniversity of California, San Diego
    DepartmentPharmacology
    Address9500 Gilman Drive #0636
    CA La Jolla 92093
    Phone858-822-2376
    vCardDownload vCard

      Collapse Biography 
      Collapse Education and Training
      McGill University, Montreal, CanadaB.ScBiochemistry
      Stanford University, Stanford, CAPh.D.Developmental Biology
      Collapse Awards and Honors
      2017  - 2022Rita Allen Foundation Milton E. Cassel Scholar Award
      2017  - 2020Klingenstein Simons Fellowship Award in Neuroscience
      2013  - 2016Takeda Pharmaceuticals New Frontiers Sceince Awardee
      2015American Association of Anatomists Young Investigator Award

      Collapse Overview 
      Collapse Overview
      Our goal is to understand the molecular mechanisms that regulate blood-brain barrier (BBB) function during health and disease. The BBB is a specialized structure formed by the blood vessels in the central nervous system (CNS) that is critical for proper brain function as well as protecting the brain from injury and disease. In most tissues the blood vessels are leaky allowing a relatively free flow of molecules and ions from blood into tissues, whereas in the brain, the blood vessels tightly restrict the movement of molecules and ions between the blood and the brain. This barrier clamps the extracellular environment allowing for proper neuronal communication while also limiting toxins, pathogens and even the body’s own immune system from entering and damaging the CNS. Breakdown of the BBB occurs during stroke, edema, brain trauma, and multiple sclerosis and is a major component of the symptoms and progression of these diseases. Furthermore, the BBB not only impedes the movement of toxins and pathogens, but also inhibits the delivery of potential therapeutic agents to the CNS.

      We use a combination of cellular, molecular and genetic approaches to understand the mechanisms of BBB formation and function. We are interested in asking:
      What are the molecules in CNS vascular cells that form the BBB?
      What are the mechanisms that lead to BBB disruption during disease?
      How does the BBB regulate brain function and behavior?

      The goal of our work is to elucidate these mechanisms, such that we will be able to develop therapeutics to rebuild the barrier to treat neurological diseases, as well as methods to bypass the barrier to deliver drugs to the CNS.


      Collapse Research 
      Collapse Research Activities and Funding
      Milton E. Cassel Award
      Rita Allen Foundation Sep 1, 2017 - Aug 31, 2022
      Role: Principal Investigator
      Klingenstein-Simons Fellowship Award in Neuroscience
      Klingenstein Foundation Jul 1, 2017 - Jun 30, 2020
      Mechanisms of blood-brain barrier disruption during neuroinflammation
      NMSS RG-1501-02549Oct 1, 2015 - Sep 30, 2018
      Role: Principal Investigator
      Regulation of blood-brain barrier formation and function by EHDs
      NIH R01NS091281Sep 30, 2015 - Jul 31, 2020
      Role: Principal Investigator
      RXRalpha and PPARdelta Signaling as Novel Regulators of the Blood-Brain Barrier
      NIH 1R01NS083688-01Jun 1, 2013 - May 31, 2018
      Role: Co-Investigator

      Collapse ORNG Applications 
      Collapse Websites

      Collapse Bibliographic 
      Collapse Publications
      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
      List All   |   Timeline
      1. Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV. Activation of RARa, RAR?, or RXRa Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Biotechnol J. 2017 Sep 28. PMID: 28960887.
        View in: PubMed
      2. Daneman R, Engelhardt B. Brain barriers in health and disease. Neurobiol Dis. 2017 Nov; 107:1-3. PMID: 28552387.
        View in: PubMed
      3. Goetzl EJ, Schwartz JB, Mustapic M, Lobach IV, Daneman R, Abner EL, Jicha GA. Altered cargo proteins of human plasma endothelial cell-derived exosomes in atherosclerotic cerebrovascular disease. FASEB J. 2017 May 05. PMID: 28476896.
        View in: PubMed
      4. Meng Y, Wiseman JA, Nemtsova Y, Moore DF, Guevarra J, Reuhl K, Banks WA, Daneman R, Sleat DE, Lobel P. A Basic ApoE-Based Peptide Mediator to Deliver Proteins across the Blood-Brain Barrier: Long-Term Efficacy, Toxicity, and Mechanism. Mol Ther. 2017 Jul 05; 25(7):1531-1543. PMID: 28456380.
        View in: PubMed
      5. Daneman R. Finding NMO. Neurol Neuroimmunol Neuroinflamm. 2017 Jan; 4(1):e313. PMID: 28018944.
        View in: PubMed
      6. Frezel N, Sohet F, Daneman R, Basbaum AI, Braz JM. Peripheral and central neuronal ATF3 precedes CD4+ T-cell infiltration in EAE. Exp Neurol. 2016 Sep; 283(Pt A):224-34. PMID: 27343802.
        View in: PubMed
      7. Tsai HH, Niu J, Munji R, Davalos D, Chang J, Zhang H, Tien AC, Kuo CJ, Chan JR, Daneman R, Fancy SP. Oligodendrocyte precursors migrate along vasculature in the developing nervous system. Science. 2016 Jan 22; 351(6271):379-84. PMID: 26798014.
        View in: PubMed
      8. Garbelli R, de Bock F, Medici V, Rousset MC, Villani F, Boussadia B, Arango-Lievano M, Jeanneteau F, Daneman R, Bartolomei F, Marchi N. PDGFRß(+) cells in human and experimental neuro-vascular dysplasia and seizures. Neuroscience. 2015 Oct 15; 306:18-27. PMID: 26283024.
        View in: PubMed
      9. Blanchette M, Daneman R. Formation and maintenance of the BBB. Mech Dev. 2015 Nov; 138 Pt 1:8-16. PMID: 26215350.
        View in: PubMed
      10. Sohet F, Lin C, Munji RN, Lee SY, Ruderisch N, Soung A, Arnold TD, Derugin N, Vexler ZS, Yen FT, Daneman R. LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation. J Cell Biol. 2015 Mar 16; 208(6):703-11. PMID: 25753034; PMCID: PMC4362448.
      11. Daneman R, Prat A. The blood-brain barrier. Cold Spring Harb Perspect Biol. 2015 Jan 05; 7(1):a020412. PMID: 25561720.
        View in: PubMed
      12. Nacer A, Movila A, Sohet F, Girgis NM, Gundra UM, Loke P, Daneman R, Frevert U. Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier. PLoS Pathog. 2014 Dec; 10(12):e1004528. PMID: 25474413; PMCID: PMC4256476.
      13. Huntley MA, Bien-Ly N, Daneman R, Watts RJ. Dissecting gene expression at the blood-brain barrier. Front Neurosci. 2014; 8:355. PMID: 25414634; PMCID: PMC4222230.
      14. Rosen SD, Daneman R. High endothelial venules through a transcriptomics lens. Nat Immunol. 2014 Oct; 15(10):906-8. PMID: 25232814.
        View in: PubMed
      15. Zhang Y, Chen K, Sloan SA, Bennett ML, Scholze AR, O'Keeffe S, Phatnani HP, Guarnieri P, Caneda C, Ruderisch N, Deng S, Liddelow SA, Zhang C, Daneman R, Maniatis T, Barres BA, Wu JQ. An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J Neurosci. 2014 Sep 03; 34(36):11929-47. PMID: 25186741; PMCID: PMC4152602.
      16. Yuen TJ, Silbereis JC, Griveau A, Chang SM, Daneman R, Fancy SPJ, Zahed H, Maltepe E, Rowitch DH. Oligodendrocyte-encoded HIF function couples postnatal myelination and white matter angiogenesis. Cell. 2014 Jul 17; 158(2):383-396. PMID: 25018103; PMCID: PMC4149873.
      17. Steketee MB, Oboudiyat C, Daneman R, Trakhtenberg E, Lamoureux P, Weinstein JE, Heidemann S, Barres BA, Goldberg JL. Regulation of intrinsic axon growth ability at retinal ganglion cell growth cones. Invest Ophthalmol Vis Sci. 2014 Jun 06; 55(7):4369-77. PMID: 24906860; PMCID: PMC4102390.
      18. Zhou L, Sohet F, Daneman R. Purification and culture of central nervous system pericytes. Cold Spring Harb Protoc. 2014 Jun 02; 2014(6):581-3. PMID: 24890216.
        View in: PubMed
      19. Zhou L, Sohet F, Daneman R. Purification of pericytes from rodent optic nerve by immunopanning. Cold Spring Harb Protoc. 2014 Jun 02; 2014(6):608-17. PMID: 24890207.
        View in: PubMed
      20. Zhou L, Sohet F, Daneman R. Purification of endothelial cells from rodent brain by immunopanning. Cold Spring Harb Protoc. 2014 Jan 01; 2014(1):65-77. PMID: 24371317.
        View in: PubMed
      21. Zhou L, Sohet F, Daneman R. Purification and culture of central nervous system endothelial cells. Cold Spring Harb Protoc. 2014 Jan 01; 2014(1):44-6. PMID: 24371313.
        View in: PubMed
      22. Obermeier B, Daneman R, Ransohoff RM. Development, maintenance and disruption of the blood-brain barrier. Nat Med. 2013 Dec; 19(12):1584-96. PMID: 24309662; PMCID: PMC4080800.
      23. Siegenthaler JA, Sohet F, Daneman R. 'Sealing off the CNS': cellular and molecular regulation of blood-brain barriergenesis. Curr Opin Neurobiol. 2013 Dec; 23(6):1057-64. PMID: 23867075; PMCID: PMC4061913.
      24. Siegenthaler JA, Choe Y, Patterson KP, Hsieh I, Li D, Jaminet SC, Daneman R, Kume T, Huang EJ, Pleasure SJ. Foxc1 is required by pericytes during fetal brain angiogenesis. Biol Open. 2013 Jul 15; 2(7):647-59. PMID: 23862012; PMCID: PMC3711032.
      25. Saunders NR, Daneman R, Dziegielewska KM, Liddelow SA. Transporters of the blood-brain and blood-CSF interfaces in development and in the adult. Mol Aspects Med. 2013 Apr-Jun; 34(2-3):742-52. PMID: 23506907.
        View in: PubMed
      26. Sohet F, Daneman R. Genetic mouse models to study blood-brain barrier development and function. Fluids Barriers CNS. 2013 Jan 10; 10(1):3. PMID: 23305182; PMCID: PMC3675378.
      27. Daneman R. The blood-brain barrier in health and disease. Ann Neurol. 2012 Nov; 72(5):648-72. PMID: 23280789.
        View in: PubMed
      28. Fernández-López D, Faustino J, Daneman R, Zhou L, Lee SY, Derugin N, Wendland MF, Vexler ZS. Blood-brain barrier permeability is increased after acute adult stroke but not neonatal stroke in the rat. J Neurosci. 2012 Jul 11; 32(28):9588-600. PMID: 22787045; PMCID: PMC3539825.
      29. Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA. Development of a method for the purification and culture of rodent astrocytes. Neuron. 2011 Sep 08; 71(5):799-811. PMID: 21903074; PMCID: PMC3172573.
      30. Crespo O, Kang SC, Daneman R, Lindstrom TM, Ho PP, Sobel RA, Steinman L, Robinson WH. Tyrosine kinase inhibitors ameliorate autoimmune encephalomyelitis in a mouse model of multiple sclerosis. J Clin Immunol. 2011 Dec; 31(6):1010-20. PMID: 21847523; PMCID: PMC3225802.
      31. Daneman R, Zhou L, Agalliu D, Cahoy JD, Kaushal A, Barres BA. The mouse blood-brain barrier transcriptome: a new resource for understanding the development and function of brain endothelial cells. PLoS One. 2010 Oct 29; 5(10):e13741. PMID: 21060791; PMCID: PMC2966423.
      32. Daneman R, Zhou L, Kebede AA, Barres BA. Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature. 2010 Nov 25; 468(7323):562-6. PMID: 20944625; PMCID: PMC3241506.
      33. Daneman R, Rescigno M. The gut immune barrier and the blood-brain barrier: are they so different? Immunity. 2009 Nov 20; 31(5):722-35. PMID: 19836264.
        View in: PubMed
      34. Daneman R, Agalliu D, Zhou L, Kuhnert F, Kuo CJ, Barres BA. Wnt/beta-catenin signaling is required for CNS, but not non-CNS, angiogenesis. Proc Natl Acad Sci U S A. 2009 Jan 13; 106(2):641-6. PMID: 19129494; PMCID: PMC2626756.
      35. Dugas JC, Mandemakers W, Rogers M, Ibrahim A, Daneman R, Barres BA. A novel purification method for CNS projection neurons leads to the identification of brain vascular cells as a source of trophic support for corticospinal motor neurons. J Neurosci. 2008 Aug 13; 28(33):8294-305. PMID: 18701692; PMCID: PMC2567869.
      36. Daneman R, Barres BA. The blood-brain barrier--lessons from moody flies. Cell. 2005 Oct 07; 123(1):9-12. PMID: 16213208.
        View in: PubMed
      37. Morin X, Daneman R, Zavortink M, Chia W. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc Natl Acad Sci U S A. 2001 Dec 18; 98(26):15050-5. PMID: 11742088; PMCID: PMC64981.
      38. Jankowski A, Kim JH, Collins RF, Daneman R, Walton P, Grinstein S. In situ measurements of the pH of mammalian peroxisomes using the fluorescent protein pHluorin. J Biol Chem. 2001 Dec 28; 276(52):48748-53. PMID: 11641408.
        View in: PubMed
      39. Bailey B, Daneman R, Daneman N, Mayer JM, Koren G. Discrepancy between CYP2D6 phenotype and genotype derived from post-mortem dextromethorphan blood level. Forensic Sci Int. 2000 May 08; 110(1):61-70. PMID: 10802201.
        View in: PubMed
      40. Fassos FF, Berkovitch M, Daneman N, Koren L, Cameron R, Klein J, Falcitelli C, St Louis P, Daneman R, Koren G. The efficacy of diazepam in the treatment of acute iron overload in rats. Can J Physiol Pharmacol. 1998 Sep; 76(9):895-9. PMID: 10066140.
        View in: PubMed
      41. Kim JH, Johannes L, Goud B, Antony C, Lingwood CA, Daneman R, Grinstein S. Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release. Proc Natl Acad Sci U S A. 1998 Mar 17; 95(6):2997-3002. PMID: 9501204; PMCID: PMC19683.
      42. Theis JG, Koren G, Daneman R, Sherwin AL, Menzano E, Cortez M, Hwang P. Interactions of clobazam with conventional antiepileptics in children. J Child Neurol. 1997 Apr; 12(3):208-13. PMID: 9130097.
        View in: PubMed
      43. Fassos FF, Berkovitch M, Daneman N, Koren L, Cameron RG, Klein J, Falcitelli C, St Louis P, Daneman R, Koren G. Efficacy of deferiprone in the treatment of acute iron intoxication in rats. J Toxicol Clin Toxicol. 1996; 34(3):279-87. PMID: 8667465.
        View in: PubMed
      Richard's Networks
      Concepts
      Derived automatically from this person's publications.
      _
      Co-Authors
      People in Profiles who have published with this person.
      _
      Similar People
      People who share similar concepts with this person.
      _
      Same Department
      Physical Neighbors
      People whose addresses are nearby this person.
      _