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Richard Daneman

Title(s)Associate Professor, Pharmacology
SchoolVc-health Sciences-schools
Address9500 Gilman Drive #0636
La Jolla CA 92093
Phone8/8-22--2376
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    Collapse Biography 
    Collapse Education and Training
    McGill University, Montreal, CanadaB.ScBiochemistry
    Stanford University, Stanford, CAPh.D.Developmental Biology
    Collapse Awards and Honors
    2017  - 2022Rita Allen Foundation Milton E. Cassel Scholar Award
    2017  - 2020Klingenstein Simons Fellowship Award in Neuroscience
    2013  - 2016Takeda Pharmaceuticals New Frontiers Sceince Awardee
    2015American Association of Anatomists Young Investigator Award

    Collapse Overview 
    Collapse Overview
    Our goal is to understand the molecular mechanisms that regulate blood-brain barrier (BBB) function during health and disease. The BBB is a specialized structure formed by the blood vessels in the central nervous system (CNS) that is critical for proper brain function as well as protecting the brain from injury and disease. In most tissues the blood vessels are leaky allowing a relatively free flow of molecules and ions from blood into tissues, whereas in the brain, the blood vessels tightly restrict the movement of molecules and ions between the blood and the brain. This barrier clamps the extracellular environment allowing for proper neuronal communication while also limiting toxins, pathogens and even the body’s own immune system from entering and damaging the CNS. Breakdown of the BBB occurs during stroke, edema, brain trauma, and multiple sclerosis and is a major component of the symptoms and progression of these diseases. Furthermore, the BBB not only impedes the movement of toxins and pathogens, but also inhibits the delivery of potential therapeutic agents to the CNS.

    We use a combination of cellular, molecular and genetic approaches to understand the mechanisms of BBB formation and function. We are interested in asking:
    What are the molecules in CNS vascular cells that form the BBB?
    What are the mechanisms that lead to BBB disruption during disease?
    How does the BBB regulate brain function and behavior?

    The goal of our work is to elucidate these mechanisms, such that we will be able to develop therapeutics to rebuild the barrier to treat neurological diseases, as well as methods to bypass the barrier to deliver drugs to the CNS.

    Collapse Research 
    Collapse Research Activities and Funding
    Blood-brain barrier monoamine metabolism regulation of social behavior
    NIH/NIMH R01MH121761Jun 1, 2020 - Mar 31, 2022
    Role: Principal Investigator
    Examining the role of perivascular fibroblasts in cerebral amyloid angiopathy during Alzheimers disease
    NIH/NIA R21AG062918Apr 1, 2019 - Jan 31, 2021
    Role: Principal Investigator
    Milton E. Cassel Award
    Rita Allen Foundation Sep 1, 2017 - Aug 31, 2022
    Role: Principal Investigator
    Klingenstein-Simons Fellowship Award in Neuroscience
    Klingenstein Foundation Jul 1, 2017 - Jun 30, 2020
    Mechanisms of blood-brain barrier disruption during neuroinflammation
    NMSS RG-1501-02549Oct 1, 2015 - Sep 30, 2018
    Role: Principal Investigator
    Regulation of blood-brain barrier formation and function by EHDs
    NIH/NINDS R01NS091281Sep 30, 2015 - Jul 31, 2020
    Role: Principal Investigator
    RXRalpha and PPARdelta Signaling as Novel Regulators of the Blood-Brain Barrier
    NIH 1R01NS083688-01Jun 1, 2013 - May 31, 2018
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Websites

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Multidimensional Proteome Profiling of Blood-Brain Barrier Perturbation by Group B Streptococcus. mSystems. 2020 Aug 25; 5(4). Campeau A, Mills RH, Blanchette M, Bajc K, Malfavon M, Munji RN, Deng L, Hancock B, Patras KA, Olson J, Nizet V, Daneman R, Doran K, Gonzalez DJ. PMID: 32843537.
      View in: PubMed   Mentions:
    2. Unexpected amount of blood-borne protein enters the young brain. Nature. 2020 07; 583(7816):362-363. Munji RN, Daneman R. PMID: 32661413.
      View in: PubMed   Mentions:    Fields:    
    3. The blood-brain barrier in health and disease: Important unanswered questions. J Exp Med. 2020 Apr 06; 217(4). Profaci CP, Munji RN, Pulido RS, Daneman R. PMID: 32211826.
      View in: PubMed   Mentions: 2     Fields:    
    4. Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids. PLoS One. 2019; 14(11):e0225206. Huang Q, Chan KY, Tobey IG, Chan YA, Poterba T, Boutros CL, Balazs AB, Daneman R, Bloom JM, Seed C, Deverman BE. PMID: 31725765.
      View in: PubMed   Mentions: 7     Fields:    Translation:HumansAnimalsCells
    5. Profiling the mouse brain endothelial transcriptome in health and disease models reveals a core blood-brain barrier dysfunction module. Nat Neurosci. 2019 11; 22(11):1892-1902. Munji RN, Soung AL, Weiner GA, Sohet F, Semple BD, Trivedi A, Gimlin K, Kotoda M, Korai M, Aydin S, Batugal A, Cabangcala AC, Schupp PG, Oldham MC, Hashimoto T, Noble-Haeusslein LJ, Daneman R. PMID: 31611708.
      View in: PubMed   Mentions: 10     Fields:    Translation:AnimalsCells
    6. Cholinergic neural activity directs retinal layer-specific angiogenesis and blood retinal barrier formation. Nat Commun. 2019 06 06; 10(1):2477. Weiner GA, Shah SH, Angelopoulos CM, Bartakova AB, Pulido RS, Murphy A, Nudleman E, Daneman R, Goldberg JL. PMID: 31171770.
      View in: PubMed   Mentions: 1     Fields:    Translation:AnimalsCells
    7. Human pluripotent stem cell-derived brain pericyte-like cells induce blood-brain barrier properties. Sci Adv. 2019 03; 5(3):eaau7375. Stebbins MJ, Gastfriend BD, Canfield SG, Lee MS, Richards D, Faubion MG, Li WJ, Daneman R, Palecek SP, Shusta EV. PMID: 30891496.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansAnimalsCells
    8. The amazing brain drain. J Exp Med. 2017 12 04; 214(12):3469-3470. Blanchette M, Daneman R. PMID: 29162645.
      View in: PubMed   Mentions:    Fields:    Translation:AnimalsCells
    9. Corrigendum: Purification of Endothelial Cells from Rodent Brain by Immunopanning. Cold Spring Harb Protoc. 2017 11 01; 2017(11). Zhou L, Sohet F, Daneman R. PMID: 29093198.
      View in: PubMed   Mentions:    Fields:    
    10. Evolutionarily Conserved Roles for Blood-Brain Barrier Xenobiotic Transporters in Endogenous Steroid Partitioning and Behavior. Cell Rep. 2017 Oct 31; 21(5):1304-1316. Hindle SJ, Munji RN, Dolghih E, Gaskins G, Orng S, Ishimoto H, Soung A, DeSalvo M, Kitamoto T, Keiser MJ, Jacobson MP, Daneman R, Bainton RJ. PMID: 29091768.
      View in: PubMed   Mentions: 5     Fields:    Translation:AnimalsCells
    11. Activation of RARa, RAR?, or RXRa Increases Barrier Tightness in Human Induced Pluripotent Stem Cell-Derived Brain Endothelial Cells. Biotechnol J. 2018 Feb; 13(2). Stebbins MJ, Lippmann ES, Faubion MG, Daneman R, Palecek SP, Shusta EV. PMID: 28960887.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansCells
    12. Brain barriers in health and disease. Neurobiol Dis. 2017 11; 107:1-3. Daneman R, Engelhardt B. PMID: 28552387.
      View in: PubMed   Mentions: 4     Fields:    Translation:Humans
    13. Altered cargo proteins of human plasma endothelial cell-derived exosomes in atherosclerotic cerebrovascular disease. FASEB J. 2017 08; 31(8):3689-3694. Goetzl EJ, Schwartz JB, Mustapic M, Lobach IV, Daneman R, Abner EL, Jicha GA. PMID: 28476896.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansCells
    14. A Basic ApoE-Based Peptide Mediator to Deliver Proteins across the Blood-Brain Barrier: Long-Term Efficacy, Toxicity, and Mechanism. Mol Ther. 2017 07 05; 25(7):1531-1543. Meng Y, Wiseman JA, Nemtsova Y, Moore DF, Guevarra J, Reuhl K, Banks WA, Daneman R, Sleat DE, Lobel P. PMID: 28456380.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansAnimalsCells
    15. Finding NMO. Neurol Neuroimmunol Neuroinflamm. 2017 Jan; 4(1):e313. Daneman R. PMID: 28018944.
      View in: PubMed   Mentions: 1     Fields:    
    16. Peripheral and central neuronal ATF3 precedes CD4+ T-cell infiltration in EAE. Exp Neurol. 2016 09; 283(Pt A):224-34. Frezel N, Sohet F, Daneman R, Basbaum AI, Braz JM. PMID: 27343802.
      View in: PubMed   Mentions: 7     Fields:    Translation:AnimalsCells
    17. Oligodendrocyte precursors migrate along vasculature in the developing nervous system. Science. 2016 Jan 22; 351(6271):379-84. Tsai HH, Niu J, Munji R, Davalos D, Chang J, Zhang H, Tien AC, Kuo CJ, Chan JR, Daneman R, Fancy SP. PMID: 26798014.
      View in: PubMed   Mentions: 61     Fields:    Translation:HumansAnimalsCells
    18. PDGFRß(+) cells in human and experimental neuro-vascular dysplasia and seizures. Neuroscience. 2015 Oct 15; 306:18-27. Garbelli R, de Bock F, Medici V, Rousset MC, Villani F, Boussadia B, Arango-Lievano M, Jeanneteau F, Daneman R, Bartolomei F, Marchi N. PMID: 26283024.
      View in: PubMed   Mentions: 4     Fields:    Translation:HumansAnimalsCells
    19. Formation and maintenance of the BBB. Mech Dev. 2015 Nov; 138 Pt 1:8-16. Blanchette M, Daneman R. PMID: 26215350.
      View in: PubMed   Mentions: 30     Fields:    Translation:HumansAnimalsCells
    20. LSR/angulin-1 is a tricellular tight junction protein involved in blood-brain barrier formation. J Cell Biol. 2015 Mar 16; 208(6):703-11. Sohet F, Lin C, Munji RN, Lee SY, Ruderisch N, Soung A, Arnold TD, Derugin N, Vexler ZS, Yen FT, Daneman R. PMID: 25753034.
      View in: PubMed   Mentions: 17     Fields:    Translation:AnimalsCells
    21. The blood-brain barrier. Cold Spring Harb Perspect Biol. 2015 Jan 05; 7(1):a020412. Daneman R, Prat A. PMID: 25561720.
      View in: PubMed   Mentions: 128     Fields:    Translation:HumansCells
    22. Experimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier. PLoS Pathog. 2014 Dec; 10(12):e1004528. Nacer A, Movila A, Sohet F, Girgis NM, Gundra UM, Loke P, Daneman R, Frevert U. PMID: 25474413.
      View in: PubMed   Mentions: 29     Fields:    Translation:HumansAnimalsCells
    23. Dissecting gene expression at the blood-brain barrier. Front Neurosci. 2014; 8:355. Huntley MA, Bien-Ly N, Daneman R, Watts RJ. PMID: 25414634.
      View in: PubMed   Mentions:
    24. High endothelial venules through a transcriptomics lens. Nat Immunol. 2014 Oct; 15(10):906-8. Rosen SD, Daneman R. PMID: 25232814.
      View in: PubMed   Mentions: 1     Fields:    Translation:AnimalsCells
    25. An RNA-sequencing transcriptome and splicing database of glia, neurons, and vascular cells of the cerebral cortex. J Neurosci. 2014 Sep 03; 34(36):11929-47. Zhang Y, Chen K, Sloan SA, Bennett ML, Scholze AR, O'Keeffe S, Phatnani HP, Guarnieri P, Caneda C, Ruderisch N, Deng S, Liddelow SA, Zhang C, Daneman R, Maniatis T, Barres BA, Wu JQ. PMID: 25186741.
      View in: PubMed   Mentions: 987     Fields:    Translation:AnimalsCells
    26. Oligodendrocyte-encoded HIF function couples postnatal myelination and white matter angiogenesis. Cell. 2014 Jul 17; 158(2):383-396. Yuen TJ, Silbereis JC, Griveau A, Chang SM, Daneman R, Fancy SPJ, Zahed H, Maltepe E, Rowitch DH. PMID: 25018103.
      View in: PubMed   Mentions: 84     Fields:    Translation:AnimalsCells
    27. Regulation of intrinsic axon growth ability at retinal ganglion cell growth cones. Invest Ophthalmol Vis Sci. 2014 Jun 06; 55(7):4369-77. Steketee MB, Oboudiyat C, Daneman R, Trakhtenberg E, Lamoureux P, Weinstein JE, Heidemann S, Barres BA, Goldberg JL. PMID: 24906860.
      View in: PubMed   Mentions: 11     Fields:    Translation:AnimalsCells
    28. Purification and culture of central nervous system pericytes. Cold Spring Harb Protoc. 2014 Jun 02; 2014(6):581-3. Zhou L, Sohet F, Daneman R. PMID: 24890216.
      View in: PubMed   Mentions: 2     Fields:    Translation:AnimalsCells
    29. Purification of pericytes from rodent optic nerve by immunopanning. Cold Spring Harb Protoc. 2014 Jun 02; 2014(6):608-17. Zhou L, Sohet F, Daneman R. PMID: 24890207.
      View in: PubMed   Mentions: 1     Fields:    Translation:AnimalsCells
    30. Purification of endothelial cells from rodent brain by immunopanning. Cold Spring Harb Protoc. 2014 Jan 01; 2014(1):65-77. Zhou L, Sohet F, Daneman R. PMID: 24371317.
      View in: PubMed   Mentions: 4     Fields:    Translation:AnimalsCells
    31. Purification and culture of central nervous system endothelial cells. Cold Spring Harb Protoc. 2014 Jan 01; 2014(1):44-6. Zhou L, Sohet F, Daneman R. PMID: 24371313.
      View in: PubMed   Mentions: 1     Fields:    Translation:AnimalsCells
    32. Development, maintenance and disruption of the blood-brain barrier. Nat Med. 2013 Dec; 19(12):1584-96. Obermeier B, Daneman R, Ransohoff RM. PMID: 24309662.
      View in: PubMed   Mentions: 320     Fields:    Translation:HumansAnimalsCells
    33. 'Sealing off the CNS': cellular and molecular regulation of blood-brain barriergenesis. Curr Opin Neurobiol. 2013 Dec; 23(6):1057-64. Siegenthaler JA, Sohet F, Daneman R. PMID: 23867075.
      View in: PubMed   Mentions: 28     Fields:    Translation:HumansAnimals
    34. Foxc1 is required by pericytes during fetal brain angiogenesis. Biol Open. 2013 Jul 15; 2(7):647-59. Siegenthaler JA, Choe Y, Patterson KP, Hsieh I, Li D, Jaminet SC, Daneman R, Kume T, Huang EJ, Pleasure SJ. PMID: 23862012.
      View in: PubMed   Mentions:
    35. Transporters of the blood-brain and blood-CSF interfaces in development and in the adult. Mol Aspects Med. 2013 Apr-Jun; 34(2-3):742-52. Saunders NR, Daneman R, Dziegielewska KM, Liddelow SA. PMID: 23506907.
      View in: PubMed   Mentions: 37     Fields:    Translation:HumansCells
    36. Genetic mouse models to study blood-brain barrier development and function. Fluids Barriers CNS. 2013 Jan 10; 10(1):3. Sohet F, Daneman R. PMID: 23305182.
      View in: PubMed   Mentions: 13     Fields:    
    37. The blood-brain barrier in health and disease. Ann Neurol. 2012 Nov; 72(5):648-72. Daneman R. PMID: 23280789.
      View in: PubMed   Mentions: 97     Fields:    Translation:Humans
    38. Blood-brain barrier permeability is increased after acute adult stroke but not neonatal stroke in the rat. J Neurosci. 2012 Jul 11; 32(28):9588-600. Fernández-López D, Faustino J, Daneman R, Zhou L, Lee SY, Derugin N, Wendland MF, Vexler ZS. PMID: 22787045.
      View in: PubMed   Mentions: 64     Fields:    Translation:AnimalsCells
    39. Development of a method for the purification and culture of rodent astrocytes. Neuron. 2011 Sep 08; 71(5):799-811. Foo LC, Allen NJ, Bushong EA, Ventura PB, Chung WS, Zhou L, Cahoy JD, Daneman R, Zong H, Ellisman MH, Barres BA. PMID: 21903074.
      View in: PubMed   Mentions: 99     Fields:    Translation:AnimalsCells
    40. Tyrosine kinase inhibitors ameliorate autoimmune encephalomyelitis in a mouse model of multiple sclerosis. J Clin Immunol. 2011 Dec; 31(6):1010-20. Crespo O, Kang SC, Daneman R, Lindstrom TM, Ho PP, Sobel RA, Steinman L, Robinson WH. PMID: 21847523.
      View in: PubMed   Mentions: 20     Fields:    Translation:HumansAnimalsCells
    41. The mouse blood-brain barrier transcriptome: a new resource for understanding the development and function of brain endothelial cells. PLoS One. 2010 Oct 29; 5(10):e13741. Daneman R, Zhou L, Agalliu D, Cahoy JD, Kaushal A, Barres BA. PMID: 21060791.
      View in: PubMed   Mentions: 159     Fields:    Translation:Animals
    42. Pericytes are required for blood-brain barrier integrity during embryogenesis. Nature. 2010 Nov 25; 468(7323):562-6. Daneman R, Zhou L, Kebede AA, Barres BA. PMID: 20944625.
      View in: PubMed   Mentions: 500     Fields:    Translation:AnimalsCells
    43. The gut immune barrier and the blood-brain barrier: are they so different? Immunity. 2009 Nov 20; 31(5):722-35. Daneman R, Rescigno M. PMID: 19836264.
      View in: PubMed   Mentions: 23     Fields:    Translation:Humans
    44. Wnt/beta-catenin signaling is required for CNS, but not non-CNS, angiogenesis. Proc Natl Acad Sci U S A. 2009 Jan 13; 106(2):641-6. Daneman R, Agalliu D, Zhou L, Kuhnert F, Kuo CJ, Barres BA. PMID: 19129494.
      View in: PubMed   Mentions: 210     Fields:    Translation:AnimalsCells
    45. A novel purification method for CNS projection neurons leads to the identification of brain vascular cells as a source of trophic support for corticospinal motor neurons. J Neurosci. 2008 Aug 13; 28(33):8294-305. Dugas JC, Mandemakers W, Rogers M, Ibrahim A, Daneman R, Barres BA. PMID: 18701692.
      View in: PubMed   Mentions: 50     Fields:    Translation:AnimalsCells
    46. The blood-brain barrier--lessons from moody flies. Cell. 2005 Oct 07; 123(1):9-12. Daneman R, Barres BA. PMID: 16213208.
      View in: PubMed   Mentions: 12     Fields:    Translation:HumansAnimalsCells
    47. A protein trap strategy to detect GFP-tagged proteins expressed from their endogenous loci in Drosophila. Proc Natl Acad Sci U S A. 2001 Dec 18; 98(26):15050-5. Morin X, Daneman R, Zavortink M, Chia W. PMID: 11742088.
      View in: PubMed   Mentions: 317     Fields:    Translation:AnimalsCells
    48. In situ measurements of the pH of mammalian peroxisomes using the fluorescent protein pHluorin. J Biol Chem. 2001 Dec 28; 276(52):48748-53. Jankowski A, Kim JH, Collins RF, Daneman R, Walton P, Grinstein S. PMID: 11641408.
      View in: PubMed   Mentions: 19     Fields:    Translation:HumansAnimalsCells
    49. Discrepancy between CYP2D6 phenotype and genotype derived from post-mortem dextromethorphan blood level. Forensic Sci Int. 2000 May 08; 110(1):61-70. Bailey B, Daneman R, Daneman N, Mayer JM, Koren G. PMID: 10802201.
      View in: PubMed   Mentions: 1     Fields:    Translation:HumansAnimals
    50. The efficacy of diazepam in the treatment of acute iron overload in rats. Can J Physiol Pharmacol. 1998 Sep; 76(9):895-9. Fassos FF, Berkovitch M, Daneman N, Koren L, Cameron R, Klein J, Falcitelli C, St Louis P, Daneman R, Koren G. PMID: 10066140.
      View in: PubMed   Mentions: 2     Fields:    Translation:Animals
    51. Noninvasive measurement of the pH of the endoplasmic reticulum at rest and during calcium release. Proc Natl Acad Sci U S A. 1998 Mar 17; 95(6):2997-3002. Kim JH, Johannes L, Goud B, Antony C, Lingwood CA, Daneman R, Grinstein S. PMID: 9501204.
      View in: PubMed   Mentions: 52     Fields:    Translation:HumansCells
    52. Interactions of clobazam with conventional antiepileptics in children. J Child Neurol. 1997 Apr; 12(3):208-13. Theis JG, Koren G, Daneman R, Sherwin AL, Menzano E, Cortez M, Hwang P. PMID: 9130097.
      View in: PubMed   Mentions: 10     Fields:    Translation:Humans
    53. Efficacy of deferiprone in the treatment of acute iron intoxication in rats. J Toxicol Clin Toxicol. 1996; 34(3):279-87. Fassos FF, Berkovitch M, Daneman N, Koren L, Cameron RG, Klein J, Falcitelli C, St Louis P, Daneman R, Koren G. PMID: 8667465.
      View in: PubMed   Mentions: 1     Fields:    Translation:Animals
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