Nadejda Beliakova-bethell

Title(s)Associate Adjunct Professor, Medicine
SchoolVc-health Sciences-schools
Address9500 Gilman Drive #
La Jolla CA 92093
ORCID ORCID Icon0000-0001-9765-7786 Additional info
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    Collapse Biography 
    Collapse Education and Training
    St. Petersburg State University, St. Petersburg, RussiaB.S.12/1998Cell Biology and Histology
    University of California, Irvine, Irvine, CA, USAPh.D.03/2009Virology, Molecular Biology
    University of California, San Diego, La Jolla, CA, USAPostdoctoral06/2013HIV/AIDS, Bioinformatics

    Collapse Overview 
    Collapse Overview
    Despite the success of antiretroviral therapy to control HIV infection, the latent reservoir formed by this virus remains the main barrier to a complete cure. Our laboratory studies the molecular mechanisms of HIV latency establishment and maintenance in human primary CD4+ T cells. We aim to determine how gene expression profiles in phenotypically different cells influence the proviral state and its ability to reactivate from latency. Currently, we focus on investigating the roles of long non-coding RNAs in the regulation of HIV gene expression and latency. We use the in vitro primary T cell models of HIV latency and CD4+ T cells obtained from people with HIV. Our most recent study has focused on delineating the heterogeneity of the latent reservoir cells using single-cell RNA-Seq technology coupled with immunophenotyping. Within these heterogeneous cell populations, we aim to identify molecular signatures (“a biomarker”) of latency that can define latently infected cells. This knowledge will likely lead to the development of approaches to capture the latently infected cells and, ultimately, to target them for elimination.

    Collapse Research 
    Collapse Research Activities and Funding
    The spectrum of long non-coding RNAs that regulate HIV expression and latency
    NIH R21AI162159Aug 15, 2022 - Jul 31, 2024
    Role: Principal Investigator
    Leaving, Coming, and Staying HIV Obligate Microenvironments (HOME)
    NIH P01AI169609Apr 1, 2022 - Mar 31, 2027
    Role: Co-Investigator
    Cellular and viral determinants of the persistent HIV reservoir
    Office of Research and Development, Veterans Health Administration I01 BX005285Oct 1, 2021 - Sep 30, 2025
    Role: Principal Investigator
    Immunometabolicgene expression profiles associated with depressed mood and behavioral domains inpeople with HIV
    NIH R01MH128869Sep 15, 2021 - Jun 30, 2026
    Role: Co-Investigator
    Role of viral tropism in molecular signatures of HIV latency
    NIH R56AI157755Jul 15, 2021 - Jun 30, 2022
    Role: Principal Investigator
    Opioid Impacts on T Cell Pathways and Epigenetics to Modulate HIV Integration, Latency and Reservoirs.
    NIH R61DA047039Sep 30, 2018 - Jun 30, 2021
    Role: Co-Investigator
    HIV reactivation from latency - role of CD4 T cell maturation phenotype
    Office of Research and Development, Veterans Health Administration IK2 BX002731Jul 1, 2016 - Jun 30, 2021
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Targeting noncoding RNAs to reactivate or eliminate latent HIV reservoirs. Curr Opin HIV AIDS. 2024 03 01; 19(2):47-55. Beliakova-Bethell N. PMID: 38169367; PMCID: PMC10872953.
      View in: PubMed   Mentions:    Fields:    Translation:HumansCells
    2. Single-cell RNA sequencing reveals common and unique gene expression profiles in primary CD4+ T cells latently infected with HIV under different conditions. Front Cell Infect Microbiol. 2023; 13:1286168. Zhang X, Qazi AA, Deshmukh S, Lobato Ventura R, Mukim A, Beliakova-Bethell N. PMID: 38156317; PMCID: PMC10754520.
      View in: PubMed   Mentions: 1     Fields:    Translation:HumansCells
    3. Beyond the Syndemic of Opioid Use Disorders and HIV: The Impact of Opioids on Viral Reservoirs. Viruses. 2023 08 09; 15(8). Trunfio M, Chaillon A, Beliakova-Bethell N, Deiss R, Letendre SL, Riggs PK, Higgins N, Gianella S. PMID: 37632053; PMCID: PMC10458944.
      View in: PubMed   Mentions: 2     Fields:    Translation:Humans
    4. HIV Infection Elicits Differential Transcriptomic Remodeling in CD4+ T Cells with Variable Proliferative Responses to the T Cell Receptor Stimulus. Pathogens. 2023 Mar 24; 12(4). Zhang X, Deshmukh S, Mukim A, Zhang J, Beliakova-Bethell N. PMID: 37111397; PMCID: PMC10145558.
      View in: PubMed   Mentions: 2  
    5. An Evaluation on the Role of Non-Coding RNA in HIV Transcription and Latency: A Review. HIV AIDS (Auckl). 2023; 15:115-134. Ramirez PW, Pantoja C, Beliakova-Bethell N. PMID: 36942082; PMCID: PMC10024501.
      View in: PubMed   Mentions: 5  
    6. A Camptothetin Analog, Topotecan, Promotes HIV Latency via Interference with HIV Transcription and RNA Splicing. J Virol. 2023 02 28; 97(2):e0163022. Mukim A, Smith DM, Deshmukh S, Qazi AA, Beliakova-Bethell N. PMID: 36719238; PMCID: PMC9973035.
      View in: PubMed   Mentions: 3     Fields:    Translation:HumansCells
    7. HIV-1 latency is established preferentially in minimally activated and non-dividing cells during productive infection of primary CD4 T cells. PLoS One. 2022; 17(7):e0271674. Soto PC, Terry VH, Lewinski MK, Deshmukh S, Beliakova-Bethell N, Spina CA. PMID: 35895672; PMCID: PMC9328514.
      View in: PubMed   Mentions: 7     Fields:    Translation:HumansCells
    8. Monocytic-Myeloid Derived Suppressor Cells Suppress T-Cell Responses in Recovered SARS CoV2-Infected Individuals. Front Immunol. 2022; 13:894543. Beliakova-Bethell N, Maruthai K, Xu R, Salvador LCM, Garg A. PMID: 35812392; PMCID: PMC9263272.
      View in: PubMed   Mentions: 8     Fields:    Translation:HumansCells
    9. Integrated proteomics and transcriptomics analyses identify novel cell surface markers of HIV latency. Virology. 2022 08; 573:50-58. Beliakova-Bethell N, Manousopoulou A, Deshmukh S, Mukim A, Richman DD, Garbis SD, Spina CA. PMID: 35714458; PMCID: PMC10427345.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansCells
    10. Micro RNA Targets in HIV Latency: Insights into Novel Layers of Latency Control. AIDS Res Hum Retroviruses. 2021 02; 37(2):109-121. Heinson AI, Woo J, Mukim A, White CH, Moesker B, Bosque A, Spina CA, Woelk CH, Macarthur BD, Beliakova-Bethell N. PMID: 33045840; PMCID: PMC7876363.
      View in: PubMed   Mentions: 11     Fields:    Translation:HumansCells
    11. Single-Cell Profiling Reveals Divergent, Globally Patterned Immune Responses in Murine Skin Inflammation. iScience. 2020 Oct 23; 23(10):101582. Liu Y, Cook C, Sedgewick AJ, Zhang S, Fassett MS, Ricardo-Gonzalez RR, Harirchian P, Kashem SW, Hanakawa S, Leistico JR, North JP, Taylor MA, Zhang W, Man MQ, Charruyer A, Beliakova-Bethell N, Benz SC, Ghadially R, Mauro TM, Kaplan DH, Kabashima K, Choi J, Song JS, Cho RJ, Cheng JB. PMID: 33205009; PMCID: PMC7648132.
      View in: PubMed   Mentions: 23  
    12. Long non-coding RNAs and latent HIV - A search for novel targets for latency reversal. PLoS One. 2019; 14(11):e0224879. Trypsteen W, White CH, Mukim A, Spina CA, De Spiegelaere W, Lefever S, Planelles V, Bosque A, Woelk CH, Vandekerckhove L, Beliakova-Bethell N. PMID: 31710657; PMCID: PMC6844474.
      View in: PubMed   Mentions: 15     Fields:    Translation:HumansCells
    13. Use of GapmeRs for gene expression knockdowns in human primary resting CD4+ T cells. J Immunol Methods. 2020 01; 476:112674. Abewe H, Deshmukh S, Mukim A, Beliakova-Bethell N. PMID: 31629740; PMCID: PMC6939142.
      View in: PubMed   Mentions: 2     Fields:    Translation:HumansCells
    14. Histone deacetylase inhibitors induce complex host responses that contribute to differential potencies of these compounds in HIV reactivation. J Biol Chem. 2019 04 05; 294(14):5576-5589. Beliakova-Bethell N, Mukim A, White CH, Deshmukh S, Abewe H, Richman DD, Spina CA. PMID: 30745362; PMCID: PMC6462528.
      View in: PubMed   Mentions: 14     Fields:    Translation:HumansCells
    15. Transcriptional Modulation of Human Endogenous Retroviruses in Primary CD4+ T Cells Following Vorinostat Treatment. Front Immunol. 2018; 9:603. White CH, Beliakova-Bethell N, Lada SM, Breen MS, Hurst TP, Spina CA, Richman DD, Frater J, Magiorkinis G, Woelk CH. PMID: 29706951; PMCID: PMC5906534.
      View in: PubMed   Mentions: 15     Fields:    Translation:HumansCells
    16. Relative efficacy of T cell stimuli as inducers of productive HIV-1 replication in latently infected CD4 lymphocytes from patients on suppressive cART. Virology. 2017 08; 508:127-133. Beliakova-Bethell N, Hezareh M, Wong JK, Strain MC, Lewinski MK, Richman DD, Spina CA. PMID: 28527342; PMCID: PMC5654527.
      View in: PubMed   Mentions: 14     Fields:    Translation:HumansCells
    17. Transcriptomic Analysis Implicates the p53 Signaling Pathway in the Establishment of HIV-1 Latency in Central Memory CD4 T Cells in an In Vitro Model. PLoS Pathog. 2016 Nov; 12(11):e1006026. White CH, Moesker B, Beliakova-Bethell N, Martins LJ, Spina CA, Margolis DM, Richman DD, Planelles V, Bosque A, Woelk CH. PMID: 27898737; PMCID: PMC5127598.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansCells
    18. Dose-responsive gene expression in suberoylanilide hydroxamic acid-treated resting CD4+ T cells. AIDS. 2015 Nov; 29(17):2235-44. Reardon B, Beliakova-Bethell N, Spina CA, Singhania A, Margolis DM, Richman DR, Woelk CH. PMID: 26258524; PMCID: PMC4917679.
      View in: PubMed   Mentions: 11     Fields:    Translation:HumansCells
    19. Acute psychological stress induces short-term variable immune response. Brain Behav Immun. 2016 Mar; 53:172-182. Breen MS, Beliakova-Bethell N, Mujica-Parodi LR, Carlson JM, Ensign WY, Woelk CH, Rana BK. PMID: 26476140.
      View in: PubMed   Mentions: 21     Fields:    Translation:HumansCells
    20. Mixed effects of suberoylanilide hydroxamic acid (SAHA) on the host transcriptome and proteome and their implications for HIV reactivation from latency. Antiviral Res. 2015 Nov; 123:78-85. White CH, Johnston HE, Moesker B, Manousopoulou A, Margolis DM, Richman DD, Spina CA, Garbis SD, Woelk CH, Beliakova-Bethell N. PMID: 26343910; PMCID: PMC5606336.
      View in: PubMed   Mentions: 27     Fields:    Translation:HumansCells
    21. Maraviroc intensification in patients with suppressed HIV viremia has limited effects on CD4+ T cell recovery and gene expression. Antiviral Res. 2014 Jul; 107:42-9. Beliakova-Bethell N, Jain S, Woelk CH, Witt MD, Sun X, Lada SM, Spina CA, Goicoechea M, Rought SE, Haubrich R, Dubé MP. PMID: 24769244; PMCID: PMC4116489.
      View in: PubMed   Mentions: 9     Fields:    Translation:HumansCells
    22. The effect of cell subset isolation method on gene expression in leukocytes. Cytometry A. 2014 Jan; 85(1):94-104. Beliakova-Bethell N, Massanella M, White C, Lada S, Du P, Vaida F, Blanco J, Spina CA, Woelk CH. PMID: 24115734; PMCID: PMC3975050.
      View in: PubMed   Mentions: 40     Fields:    Translation:HumansCells
    23. Differential gene expression in HIV-infected individuals following ART. Antiviral Res. 2013 Nov; 100(2):420-8. Massanella M, Singhania A, Beliakova-Bethell N, Pier R, Lada SM, White CH, Pérez-Santiago J, Blanco J, Richman DD, Little SJ, Woelk CH. PMID: 23933117; PMCID: PMC3899918.
      View in: PubMed   Mentions: 21     Fields:    Translation:Humans
    24. Suberoylanilide hydroxamic acid induces limited changes in the transcriptome of primary CD4(+) T cells. AIDS. 2013 Jan 02; 27(1):29-37. Beliakova-Bethell N, Zhang JX, Singhania A, Lee V, Terry VH, Richman DD, Spina CA, Woelk CH. PMID: 23221426; PMCID: PMC3752851.
      View in: PubMed   Mentions: 18     Fields:    Translation:HumansCells
    25. HIV downregulates interferon-stimulated genes in primary macrophages. J Interferon Cytokine Res. 2013 Feb; 33(2):90-5. Wie SH, Du P, Luong TQ, Rought SE, Beliakova-Bethell N, Lozach J, Corbeil J, Kornbluth RS, Richman DD, Woelk CH. PMID: 23276142; PMCID: PMC3573720.
      View in: PubMed   Mentions: 23     Fields:    Translation:HumansCells
    26. Sequence requirements for localization and packaging of Ty3 retroelement RNA. Virus Res. 2013 Feb; 171(2):319-31. Clemens K, Bilanchone V, Beliakova-Bethell N, Larsen LS, Nguyen K, Sandmeyer S. PMID: 23073180; PMCID: PMC3578171.
      View in: PubMed   Mentions: 8     Fields:    Translation:AnimalsCells
    27. Gene expression before HAART initiation predicts HIV-infected individuals at risk of poor CD4+ T-cell recovery. AIDS. 2010 Jan 16; 24(2):217-22. Woelk CH, Beliakova-Bethell N, Goicoechea M, Zhao Y, Du P, Rought SE, Lozach J, Pérez-Santiago J, Richman DD, Smith DM, Little SJ. PMID: 19952713; PMCID: PMC2915828.
      View in: PubMed   Mentions: 13     Fields:    Translation:HumansCells
    28. Ty3 nuclear entry is initiated by viruslike particle docking on GLFG nucleoporins. J Virol. 2009 Nov; 83(22):11914-25. Beliakova-Bethell N, Terry LJ, Bilanchone V, DaSilva R, Nagashima K, Wente SR, Sandmeyer S. PMID: 19759143; PMCID: PMC2772691.
      View in: PubMed   Mentions: 12     Fields:    Translation:AnimalsCells
    29. Ty3 nucleocapsid controls localization of particle assembly. J Virol. 2008 Mar; 82(5):2501-14. Larsen LS, Beliakova-Bethell N, Bilanchone V, Zhang M, Lamsa A, Dasilva R, Hatfield GW, Nagashima K, Sandmeyer S. PMID: 18094177; PMCID: PMC2258933.
      View in: PubMed   Mentions: 26     Fields:    Translation:AnimalsCells
    30. Ty3 capsid mutations reveal early and late functions of the amino-terminal domain. J Virol. 2007 Jul; 81(13):6957-72. Larsen LS, Zhang M, Beliakova-Bethell N, Bilanchone V, Lamsa A, Nagashima K, Najdi R, Kosaka K, Kovacevic V, Cheng J, Baldi P, Hatfield GW, Sandmeyer S. PMID: 17442718; PMCID: PMC1933270.
      View in: PubMed   Mentions: 24     Fields:    Translation:AnimalsCells
    31. Virus-like particles of the Ty3 retrotransposon assemble in association with P-body components. RNA. 2006 Jan; 12(1):94-101. Beliakova-Bethell N, Beckham C, Giddings TH, Winey M, Parker R, Sandmeyer S. PMID: 16373495; PMCID: PMC1370889.
      View in: PubMed   Mentions: 64     Fields:    Translation:AnimalsCells
    32. Retroviruses and yeast retrotransposons use overlapping sets of host genes. Genome Res. 2005 May; 15(5):641-54. Irwin B, Aye M, Baldi P, Beliakova-Bethell N, Cheng H, Dou Y, Liou W, Sandmeyer S. PMID: 15837808; PMCID: PMC1088292.
      View in: PubMed   Mentions: 59     Fields:    Translation:AnimalsCells
    33. Host factors that affect Ty3 retrotransposition in Saccharomyces cerevisiae. Genetics. 2004 Nov; 168(3):1159-76. Aye M, Irwin B, Beliakova-Bethell N, Chen E, Garrus J, Sandmeyer S. PMID: 15579677; PMCID: PMC1448793.
      View in: PubMed   Mentions: 32     Fields:    Translation:AnimalsCells
    34. Ty3 integrase is required for initiation of reverse transcription. J Virol. 2002 Mar; 76(6):2804-16. Nymark-McMahon MH, Beliakova-Bethell NS, Darlix JL, Le Grice SF, Sandmeyer SB. PMID: 11861848; PMCID: PMC135951.
      View in: PubMed   Mentions: 25     Fields:    Translation:AnimalsCells
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