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Stephen Mayfield

TitleProfessor
InstitutionUniversity of California San Diego
DepartmentMolecular Biology
Address9500 Gilman Drive #0116
La Jolla CA 92093
Phone858-822-7743
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    Collapse Research 
    Collapse Research Activities and Funding
    Production of Antibodies in Algae for Biodefense
    NIH/NIAID R01AI059614Dec 1, 2004 - Nov 30, 2009
    Role: Principal Investigator
    Protein/RNA Interactions in Translational Regulation
    NIH/NIGMS R01GM054659May 1, 1997 - Nov 30, 2006
    Role: Principal Investigator
    NUCLEAR GENES REGULATING TRANSLATION OF ORGANELLES MRNAS
    NIH/NIGMS R01GM041353Dec 1, 1988 - Nov 30, 1992
    Role: Principal Investigator
    ISOLATION OF CAROTENOID BIOSYNTHETIC GENES
    NIH/NIGMS F32GM010246Oct 3, 1985
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Ravi A, Guo S, Rasala B, Tran M, Mayfield S, Nikolov ZL. Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii. Int J Mol Sci. 2018 Feb 16; 19(2). PMID: 29462927.
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    2. Molino JVD, de Carvalho JCM, Mayfield S. Comparison of secretory signal peptides for heterologous protein expression in microalgae: Expanding the secretion portfolio for Chlamydomonas reinhardtii. PLoS One. 2018; 13(2):e0192433. PMID: 29408937.
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    3. Shurin JB, Burkart MD, Mayfield S, Smith VH. Recent progress and future challenges in algal biofuel production. F1000Res. 2016; 5. PMID: 27781084.
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    4. Beyter D, Tang PZ, Becker S, Hoang T, Bilgin D, Lim YW, Peterson TC, Mayfield S, Haerizadeh F, Shurin JB, Bafna V, McBride R. Diversity, Productivity, and Stability of an Industrial Microbial Ecosystem. Appl Environ Microbiol. 2016 Apr; 82(8):2494-2505. PMID: 26896141.
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    5. Gregory JA, Shepley-McTaggart A, Umpierrez M, Hurlburt BK, Maleki SJ, Sampson HA, Mayfield S, Berin MC. Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice. Plant Biotechnol J. 2016 07; 14(7):1541-50. PMID: 26801740.
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    6. Vinyard DJ, Sun JS, Gimpel J, Ananyev GM, Mayfield S, Charles Dismukes G. Natural isoforms of the Photosystem II D1 subunit differ in photoassembly efficiency of the water-oxidizing complex. Photosynth Res. 2016 May; 128(2):141-50. PMID: 26687161.
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    7. Gimpel JA, Henríquez V, Mayfield S. In Metabolic Engineering of Eukaryotic Microalgae: Potential and Challenges Come with Great Diversity. Front Microbiol. 2015; 6:1376. PMID: 26696985; PMCID: PMC4678203.
    8. Gimpel JA, Nour-Eldin HH, Scranton MA, Li D, Mayfield S. Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii. ACS Synth Biol. 2016 07 15; 5(7):589-96. PMID: 26214707.
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    9. Ferreira-Camargo LS, Tran M, Beld J, Burkart MD, Mayfield S. Selenocystamine improves protein accumulation in chloroplasts of eukaryotic green algae. AMB Express. 2015 Dec; 5(1):126. PMID: 26137911; PMCID: PMC4489976.
    10. Mayfield S, Golden SS. Photosynthetic bio-manufacturing: food, fuel, and medicine for the 21st century. Photosynth Res. 2015 Mar; 123(3):225-6. PMID: 25676708.
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    11. Scranton MA, Ostrand JT, Fields FJ, Mayfield S. Chlamydomonas as a model for biofuels and bio-products production. Plant J. 2015 May; 82(3):523-31. PMID: 25641390.
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    12. Patra KP, Li F, Carter D, Gregory JA, Baga S, Reed SG, Mayfield S, Vinetz JM. Alga-produced malaria transmission-blocking vaccine candidate Pfs25 formulated with a human use-compatible potent adjuvant induces high-affinity antibodies that block Plasmodium falciparum infection of mosquitoes. Infect Immun. 2015 May; 83(5):1799-808. PMID: 25690099; PMCID: PMC4399074.
    13. Specht EA, Nour-Eldin HH, Hoang KT, Mayfield S. An improved ARS2-derived nuclear reporter enhances the efficiency and ease of genetic engineering in Chlamydomonas. Biotechnol J. 2015 Mar; 10(3):473-9. PMID: 25224580.
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    14. Barrera DJ, Rosenberg JN, Chiu JG, Chang YN, Debatis M, Ngoi SM, Chang JT, Shoemaker CB, Oyler GA, Mayfield S. Algal chloroplast produced camelid VH H antitoxins are capable of neutralizing botulinum neurotoxin. Plant Biotechnol J. 2015 Jan; 13(1):117-24. PMID: 25229405; PMCID: PMC4620920.
    15. Gimpel JA, Hyun JS, Schoepp NG, Mayfield S. Production of recombinant proteins in microalgae at pilot greenhouse scale. Biotechnol Bioeng. 2015 Feb; 112(2):339-45. PMID: 25116083.
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    16. Schoepp NG, Stewart RL, Sun V, Quigley AJ, Mendola D, Mayfield S, Burkart MD. System and method for research-scale outdoor production of microalgae and cyanobacteria. Bioresour Technol. 2014 Aug; 166:273-81. PMID: 24926599.
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    17. O'Sullivan T, Saddawi-Konefka R, Gross E, Tran M, Mayfield S, Ikeda H, Bui JD. Interleukin-17D mediates tumor rejection through recruitment of natural killer cells. Cell Rep. 2014 May 22; 7(4):989-98. PMID: 24794441; PMCID: PMC4084720.
    18. Rasala BA, Chao SS, Pier M, Barrera DJ, Mayfield S. Enhanced genetic tools for engineering multigene traits into green algae. PLoS One. 2014; 9(4):e94028. PMID: 24710110; PMCID: PMC3978050.
    19. Rasala BA, Mayfield S. Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses. Photosynth Res. 2015 Mar; 123(3):227-39. PMID: 24659086.
      View in: PubMed
    20. Vinyard DJ, Gimpel J, Ananyev GM, Mayfield S, Dismukes GC. Engineered Photosystem II reaction centers optimize photochemistry versus photoprotection at different solar intensities. J Am Chem Soc. 2014 Mar 12; 136(10):4048-55. PMID: 24548276.
      View in: PubMed
    21. Specht EA, Mayfield S. Algae-based oral recombinant vaccines. Front Microbiol. 2014; 5:60. PMID: 24596570; PMCID: PMC3925837.
    22. Gregory JA, Mayfield S. Developing inexpensive malaria vaccines from plants and algae. Appl Microbiol Biotechnol. 2014 Mar; 98(5):1983-90. PMID: 24413920.
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    23. Barrera D, Gimpel J, Mayfield S. Rapid screening for the robust expression of recombinant proteins in algal plastids. Methods Mol Biol. 2014; 1132:391-9. PMID: 24599869.
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    24. Mayfield S. The Green Revolution 2.0: the potential of algae for the production of biofuels and bioproducts. Genome. 2013 Oct; 56(10):551-5. PMID: 24237335.
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    25. Tran M, Henry RE, Siefker D, Van C, Newkirk G, Kim J, Bui J, Mayfield S. Production of anti-cancer immunotoxins in algae: ribosome inactivating proteins as fusion partners. Biotechnol Bioeng. 2013 Nov; 110(11):2826-35. PMID: 23719862.
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    26. Burkart MD, Mayfield S. Biofuels for the 21st century. Curr Opin Chem Biol. 2013 Jun; 17(3):436-8. PMID: 23722079.
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    27. Gimpel JA, Specht EA, Georgianna DR, Mayfield S. Advances in microalgae engineering and synthetic biology applications for biofuel production. Curr Opin Chem Biol. 2013 Jun; 17(3):489-95. PMID: 23684717.
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    28. Gregory JA, Topol AB, Doerner DZ, Mayfield S. Alga-produced cholera toxin-Pfs25 fusion proteins as oral vaccines. Appl Environ Microbiol. 2013 Jul; 79(13):3917-25. PMID: 23603678; PMCID: PMC3697563.
    29. Rasala BA, Barrera DJ, Ng J, Plucinak TM, Rosenberg JN, Weeks DP, Oyler GA, Peterson TC, Haerizadeh F, Mayfield S. Expanding the spectral palette of fluorescent proteins for the green microalga Chlamydomonas reinhardtii. Plant J. 2013 May; 74(4):545-56. PMID: 23521393.
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    30. Vinyard DJ, Gimpel J, Ananyev GM, Cornejo MA, Golden SS, Mayfield S, Dismukes GC. Natural variants of photosystem II subunit D1 tune photochemical fitness to solar intensity. J Biol Chem. 2013 Feb 22; 288(8):5451-62. PMID: 23271739; PMCID: PMC3581373.
    31. Tran M, Van C, Barrera DJ, Pettersson PL, Peinado CD, Bui J, Mayfield S. Production of unique immunotoxin cancer therapeutics in algal chloroplasts. Proc Natl Acad Sci U S A. 2013 Jan 02; 110(1):E15-22. PMID: 23236148; PMCID: PMC3538218.
    32. Gimpel JA, Mayfield S. Analysis of heterologous regulatory and coding regions in algal chloroplasts. Appl Microbiol Biotechnol. 2013 May; 97(10):4499-510. PMID: 23179624.
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    33. Specht EA, Mayfield S. Synthetic oligonucleotide libraries reveal novel regulatory elements in Chlamydomonas chloroplast mRNAs. ACS Synth Biol. 2013 Jan 18; 2(1):34-46. PMID: 23656324.
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    34. Blatti JL, Beld J, Behnke CA, Mendez M, Mayfield S, Burkart MD. Manipulating fatty acid biosynthesis in microalgae for biofuel through protein-protein interactions. PLoS One. 2012; 7(9):e42949. PMID: 23028438.
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    35. Rasala BA, Lee PA, Shen Z, Briggs SP, Mendez M, Mayfield S. Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoS One. 2012; 7(8):e43349. PMID: 22937037; PMCID: PMC3427385.
    36. Georgianna DR, Mayfield S. Exploiting diversity and synthetic biology for the production of algal biofuels. Nature. 2012 Aug 16; 488(7411):329-35. PMID: 22895338.
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    37. Jones CS, Luong T, Hannon M, Tran M, Gregory JA, Shen Z, Briggs SP, Mayfield S. Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii. Appl Microbiol Biotechnol. 2013 Mar; 97(5):1987-95. PMID: 22592550.
      View in: PubMed
    38. Gregory JA, Li F, Tomosada LM, Cox CJ, Topol AB, Vinetz JM, Mayfield S. Algae-produced Pfs25 elicits antibodies that inhibit malaria transmission. PLoS One. 2012; 7(5):e37179. PMID: 22615931; PMCID: PMC3353897.
    39. Jones CS, Mayfield S. Steps toward a globally available malaria vaccine: harnessing the potential of algae for future low cost vaccines. Bioengineered. 2013 May-Jun; 4(3):164-7. PMID: 23090388; PMCID: PMC3669158.
    40. Jones CS, Mayfield S. Algae biofuels: versatility for the future of bioenergy. Curr Opin Biotechnol. 2012 Jun; 23(3):346-51. PMID: 22104720.
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    41. Yu WL, Ansari W, Schoepp NG, Hannon MJ, Mayfield S, Burkart MD. Modifications of the metabolic pathways of lipid and triacylglycerol production in microalgae. Microb Cell Fact. 2011 Nov 02; 10:91. PMID: 22047615; PMCID: PMC3234195.
    42. Mayfield S, Wong PK. Forum. Chemical engineering: Fuel for debate. Nature. 2011 Aug 24; 476(7361):402-3. PMID: 21866147.
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    43. Rasala BA, Muto M, Sullivan J, Mayfield S. Improved heterologous protein expression in the chloroplast of Chlamydomonas reinhardtii through promoter and 5' untranslated region optimization. Plant Biotechnol J. 2011 Aug; 9(6):674-83. PMID: 21535358.
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    44. Coragliotti AT, Beligni MV, Franklin SE, Mayfield S. Molecular factors affecting the accumulation of recombinant proteins in the Chlamydomonas reinhardtii chloroplast. Mol Biotechnol. 2011 May; 48(1):60-75. PMID: 21113690; PMCID: PMC3068253.
    45. Rasala BA, Mayfield S. The microalga Chlamydomonas reinhardtii as a platform for the production of human protein therapeutics. Bioeng Bugs. 2011 Jan-Feb; 2(1):50-4. PMID: 21636988; PMCID: PMC3127021.
    46. Hannon M, Gimpel J, Tran M, Rasala B, Mayfield S. Biofuels from algae: challenges and potential. Biofuels. 2010 Sep; 1(5):763-784. PMID: 21833344.
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    47. Specht E, Miyake-Stoner S, Mayfield S. Micro-algae come of age as a platform for recombinant protein production. Biotechnol Lett. 2010 Oct; 32(10):1373-83. PMID: 20556634; PMCID: PMC2941057.
    48. Rasala BA, Muto M, Lee PA, Jager M, Cardoso RM, Behnke CA, Kirk P, Hokanson CA, Crea R, Mendez M, Mayfield S. Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii. Plant Biotechnol J. 2010 Aug; 8(6):719-33. PMID: 20230484; PMCID: PMC2918638.
    49. Tran M, Zhou B, Pettersson PL, Gonzalez MJ, Mayfield S. Synthesis and assembly of a full-length human monoclonal antibody in algal chloroplasts. Biotechnol Bioeng. 2009 Nov 01; 104(4):663-73. PMID: 19562731.
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    50. Muto M, Henry RE, Mayfield S. Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast. BMC Biotechnol. 2009 Mar 26; 9:26. PMID: 19323825; PMCID: PMC2671499.
    51. Beligni MV, Mayfield S. Arabidopsis thaliana mutants reveal a role for CSP41a and CSP41b, two ribosome-associated endonucleases, in chloroplast ribosomal RNA metabolism. Plant Mol Biol. 2008 Jul; 67(4):389-401. PMID: 18398686.
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    52. Merchant SS, Prochnik SE, Vallon O, Harris EH, Karpowicz SJ, Witman GB, Terry A, Salamov A, Fritz-Laylin LK, Maréchal-Drouard L, Marshall WF, Qu LH, Nelson DR, Sanderfoot AA, Spalding MH, Kapitonov VV, Ren Q, Ferris P, Lindquist E, Shapiro H, Lucas SM, Grimwood J, Schmutz J, Cardol P, Cerutti H, Chanfreau G, Chen CL, Cognat V, Croft MT, Dent R, Dutcher S, Fernández E, Fukuzawa H, González-Ballester D, González-Halphen D, Hallmann A, Hanikenne M, Hippler M, Inwood W, Jabbari K, Kalanon M, Kuras R, Lefebvre PA, Lemaire SD, Lobanov AV, Lohr M, Manuell A, Meier I, Mets L, Mittag M, Mittelmeier T, Moroney JV, Moseley J, Napoli C, Nedelcu AM, Niyogi K, Novoselov SV, Paulsen IT, Pazour G, Purton S, Ral JP, Riaño-Pachón DM, Riekhof W, Rymarquis L, Schroda M, Stern D, Umen J, Willows R, Wilson N, Zimmer SL, Allmer J, Balk J, Bisova K, Chen CJ, Elias M, Gendler K, Hauser C, Lamb MR, Ledford H, Long JC, Minagawa J, Page MD, Pan J, Pootakham W, Roje S, Rose A, Stahlberg E, Terauchi AM, Yang P, Ball S, Bowler C, Dieckmann CL, Gladyshev VN, Green P, Jorgensen R, Mayfield S, Mueller-Roeber B, Rajamani S, Sayre RT, Brokstein P, Dubchak I, Goodstein D, Hornick L, Huang YW, Jhaveri J, Luo Y, Martínez D, Ngau WC, Otillar B, Poliakov A, Porter A, Szajkowski L, Werner G, Zhou K, Grigoriev IV, Rokhsar DS, Grossman AR. The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science. 2007 Oct 12; 318(5848):245-50. PMID: 17932292; PMCID: PMC2875087.
    53. Manuell AL, Quispe J, Mayfield S. Structure of the chloroplast ribosome: novel domains for translation regulation. PLoS Biol. 2007 Aug; 5(8):e209. PMID: 17683199; PMCID: PMC1939882.
    54. Manuell AL, Beligni MV, Elder JH, Siefker DT, Tran M, Weber A, McDonald TL, Mayfield S. Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast. Plant Biotechnol J. 2007 May; 5(3):402-12. PMID: 17359495.
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    55. Mayfield S, Manuell AL, Chen S, Wu J, Tran M, Siefker D, Muto M, Marin-Navarro J. Chlamydomonas reinhardtii chloroplasts as protein factories. Curr Opin Biotechnol. 2007 Apr; 18(2):126-33. PMID: 17317144.
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    56. Fletcher SP, Muto M, Mayfield S. Optimization of recombinant protein expression in the chloroplasts of green algae. Adv Exp Med Biol. 2007; 616:90-8. PMID: 18161493.
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    57. Manuell AL, Mayfield S. A bright future for Chlamydomonas. Genome Biol. 2006; 7(9):327. PMID: 16973002; PMCID: PMC1794549.
    58. Barnes D, Franklin S, Schultz J, Henry R, Brown E, Coragliotti A, Mayfield S. Contribution of 5'- and 3'-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes. Mol Genet Genomics. 2005 Dec; 274(6):625-36. PMID: 16231149.
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    59. Manuell AL, Yamaguchi K, Haynes PA, Milligan RA, Mayfield S. Composition and structure of the 80S ribosome from the green alga Chlamydomonas reinhardtii: 80S ribosomes are conserved in plants and animals. J Mol Biol. 2005 Aug 12; 351(2):266-79. PMID: 16005888.
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    60. Somanchi A, Barnes D, Mayfield S. A nuclear gene of Chlamydomonas reinhardtii, Tba1, encodes a putative oxidoreductase required for translation of the chloroplast psbA mRNA. Plant J. 2005 May; 42(3):341-52. PMID: 15842620.
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    61. Mayfield S, Franklin SE. Expression of human antibodies in eukaryotic micro-algae. Vaccine. 2005 Mar 07; 23(15):1828-32. PMID: 15734050.
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    62. Franklin SE, Mayfield S. Recent developments in the production of human therapeutic proteins in eukaryotic algae. Expert Opin Biol Ther. 2005 Feb; 5(2):225-35. PMID: 15757384.
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    63. Beligni MV, Yamaguchi K, Mayfield S. Chloroplast elongation factor ts pro-protein is an evolutionarily conserved fusion with the s1 domain-containing plastid-specific ribosomal protein-7. Plant Cell. 2004 Dec; 16(12):3357-69. PMID: 15548736; PMCID: PMC535878.
    64. Barnes D, Cohen A, Bruick RK, Kantardjieff K, Fowler S, Efuet E, Mayfield S. Identification and characterization of a novel RNA binding protein that associates with the 5'-untranslated region of the chloroplast psbA mRNA. Biochemistry. 2004 Jul 06; 43(26):8541-50. PMID: 15222765.
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    65. Franklin SE, Mayfield S. Prospects for molecular farming in the green alga Chlamydomonas. Curr Opin Plant Biol. 2004 Apr; 7(2):159-65. PMID: 15003216.
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    66. Mayfield S, Schultz J. Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast. Plant J. 2004 Feb; 37(3):449-58. PMID: 14731263.
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    67. Beligni MV, Yamaguchi K, Mayfield S. The translational apparatus of Chlamydomonas reinhardtii chloroplast. Photosynth Res. 2004; 82(3):315-25. PMID: 16143843.
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    68. Yamaguchi K, Beligni MV, Prieto S, Haynes PA, McDonald WH, Yates JR, Mayfield S. Proteomic characterization of the Chlamydomonas reinhardtii chloroplast ribosome. Identification of proteins unique to th e70 S ribosome. J Biol Chem. 2003 Sep 05; 278(36):33774-85. PMID: 12826678.
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    69. Barnes D, Mayfield S. Redox control of posttranscriptional processes in the chloroplast. Antioxid Redox Signal. 2003 Feb; 5(1):89-94. PMID: 12626120.
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    70. Mayfield S, Franklin SE, Lerner RA. Expression and assembly of a fully active antibody in algae. Proc Natl Acad Sci U S A. 2003 Jan 21; 100(2):438-42. PMID: 12522151; PMCID: PMC141013.
    71. Yamaguchi K, Prieto S, Beligni MV, Haynes PA, McDonald WH, Yates JR, Mayfield S. Proteomic characterization of the small subunit of Chlamydomonas reinhardtii chloroplast ribosome: identification of a novel S1 domain-containing protein and unusually large orthologs of bacterial S2, S3, and S5. Plant Cell. 2002 Nov; 14(11):2957-74. PMID: 12417713; PMCID: PMC152739.
    72. Kim J, Mayfield S. The active site of the thioredoxin-like domain of chloroplast protein disulfide isomerase, RB60, catalyzes the redox-regulated binding of chloroplast poly(A)-binding protein, RB47, to the 5' untranslated region of psbA mRNA. Plant Cell Physiol. 2002 Oct; 43(10):1238-43. PMID: 12407204.
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    73. Franklin S, Ngo B, Efuet E, Mayfield S. Development of a GFP reporter gene for Chlamydomonas reinhardtii chloroplast. Plant J. 2002 Jun; 30(6):733-44. PMID: 12061904.
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