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Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco. Transgenic Res. 2024 Apr 04.
Gruchow HM, Opdensteinen P, Buyel JF. PMID: 38573429.
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PubMed Mentions: Fields:
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Production of enzymes for the removal of odorous substances in plant biomass. Protein Expr Purif. 2024 Feb; 214:106379.
Opdensteinen P, Knödler M, Buyel JF. PMID: 37816475.
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Animals
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Killer to cure: Expression and production costs calculation of tobacco plant-made cancer-immune checkpoint inhibitors. Plant Biotechnol J. 2023 06; 21(6):1254-1269.
Ridgley LA, Falci Finardi N, Gengenbach BB, Opdensteinen P, Croxford Z, Ma JK, Bodman-Smith M, Buyel JF, Teh AY. PMID: 36811226; PMCID: PMC10214761.
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PubMed Mentions:
3 Fields:
Translation:
HumansAnimals
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Simple plant-based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor-targeted drug delivery and bioimaging in cancer therapy. Biotechnol Bioeng. 2023 04; 120(4):1038-1054.
Knödler M, Opdensteinen P, Sankaranarayanan RA, Morgenroth A, Buhl EM, Mottaghy FM, Buyel JF. PMID: 36539373.
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PubMed Mentions:
3 Fields:
Translation:
Humans
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The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy. Plant Biotechnol J. 2022 10; 20(10):1928-1939.
Opdensteinen P, Sperl LE, Mohamadi M, Kündgen-Redding N, Hagn F, Buyel JF. PMID: 35702941; PMCID: PMC9491462.
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PubMed Mentions:
1 Fields:
Translation:
Cells
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Reducing water uptake into BY-2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs. Biotechnol J. 2022 Oct; 17(10):e2200134.
Opdensteinen P, Buyel JF. PMID: 35762355.
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PubMed Mentions:
1 Fields:
Translation:
AnimalsCells
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Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms. Front Bioeng Biotechnol. 2021; 9:708150.
Opdensteinen P, Dietz SJ, Gengenbach BB, Buyel JF. PMID: 34621728; PMCID: PMC8490750.
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PubMed Mentions:
1
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Heterologous production of α-Carotene in Corynebacterium glutamicum using a multi-copy chromosomal integration method. Bioresour Technol. 2021 Dec; 341:125782.
Li C, Swofford CA, Rückert C, Chatzivasileiou AO, Ou RW, Opdensteinen P, Luttermann T, Zhou K, Stephanopoulos G, Jones Prather KL, Zhong-Johnson EZL, Liang S, Zheng S, Lin Y, Sinskey AJ. PMID: 34419880.
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PubMed Mentions:
6 Fields:
Translation:
HumansCells
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Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins. J Chromatogr A. 2021 Aug 30; 1652:462379.
Bernau CR, Jäpel RC, Hübbers JW, Nölting S, Opdensteinen P, Buyel JF. PMID: 34256268.
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PubMed Mentions:
1 Fields:
Translation:
HumansCells
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A combined pH and temperature precipitation step facilitates the purification of tobacco-derived recombinant proteins that are sensitive to extremes of either parameter. Biotechnol J. 2021 Apr; 16(4):e2000340.
Opdensteinen P, Lobanov A, Buyel JF. PMID: 33247609.
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PubMed Mentions:
4 Fields:
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Robot Cookies - Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression. Front Bioeng Biotechnol. 2020; 8:393.
Gengenbach BB, Opdensteinen P, Buyel JF. PMID: 32432097; PMCID: PMC7214789.
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PubMed Mentions:
13
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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. J Vis Exp. 2019 08 16; (150).
Knödler M, Rühl C, Opdensteinen P, Buyel JF. PMID: 31475984.
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PubMed Mentions:
3 Fields:
Translation:
Animals
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Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin. Biotechnol Bioeng. 2019 09; 116(9):2236-2249.
Gengenbach BB, Keil LL, Opdensteinen P, Müschen CR, Melmer G, Lentzen H, Bührmann J, Buyel JF. PMID: 31140580; PMCID: PMC6772165.
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PubMed Mentions:
20 Fields:
Translation:
AnimalsCells
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A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts. Front Bioeng Biotechnol. 2018; 6:206.
Opdensteinen P, Clodt JI, Müschen CR, Filiz V, Buyel JF. PMID: 30687700; PMCID: PMC6334625.
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PubMed Mentions:
10
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A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies. J Chromatogr A. 2018 Oct 12; 1571:55-64.
Rühl C, Knödler M, Opdensteinen P, Buyel JF. PMID: 30104060.
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PubMed Mentions:
5 Fields:
Translation:
Animals
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Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synth Biol. 2017 12 15; 6(12):2183-2190.
Purcell O, Opdensteinen P, Chen W, Lowenhaupt K, Brown A, Hermann M, Cao J, Tenhaef N, Kallweit E, Kastilan R, Sinskey AJ, Perez-Pinera P, Buyel JF, Lu TK. PMID: 28786662.
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PubMed Mentions:
5 Fields:
Translation:
HumansAnimalsCells
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Cellulose-based filter aids increase the capacity of depth filters during the downstream processing of plant-derived biopharmaceutical proteins. Biotechnol J. 2015 Apr; 10(4):584-91.
Buyel JF, Opdensteinen P, Fischer R. PMID: 25611947.
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PubMed Mentions:
10 Fields:
Translation:
Cells