Patrick Opdensteinen

Title(s)Postdoctoral Scholar, Nanoengineering
SchoolVc-academic Affairs
Address9500 Gilman Drive #
La Jolla CA 92093
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help. to make corrections and additions.
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    Altmetrics Details PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Membrane-based inverse-transition purification facilitates a rapid isolation of various spider-silk elastin-like polypeptide fusion proteins from extracts of transgenic tobacco. Transgenic Res. 2024 Apr 04. Gruchow HM, Opdensteinen P, Buyel JF. PMID: 38573429.
      View in: PubMed   Mentions:    Fields:    
    2. Production of enzymes for the removal of odorous substances in plant biomass. Protein Expr Purif. 2024 Feb; 214:106379. Opdensteinen P, Knödler M, Buyel JF. PMID: 37816475.
      View in: PubMed   Mentions:    Fields:    Translation:Animals
    3. Killer to cure: Expression and production costs calculation of tobacco plant-made cancer-immune checkpoint inhibitors. Plant Biotechnol J. 2023 06; 21(6):1254-1269. Ridgley LA, Falci Finardi N, Gengenbach BB, Opdensteinen P, Croxford Z, Ma JK, Bodman-Smith M, Buyel JF, Teh AY. PMID: 36811226; PMCID: PMC10214761.
      View in: PubMed   Mentions: 3     Fields:    Translation:HumansAnimals
    4. Simple plant-based production and purification of the assembled human ferritin heavy chain as a nanocarrier for tumor-targeted drug delivery and bioimaging in cancer therapy. Biotechnol Bioeng. 2023 04; 120(4):1038-1054. Knödler M, Opdensteinen P, Sankaranarayanan RA, Morgenroth A, Buhl EM, Mottaghy FM, Buyel JF. PMID: 36539373.
      View in: PubMed   Mentions: 3     Fields:    Translation:Humans
    5. The transient expression of recombinant proteins in plant cell packs facilitates stable isotope labelling for NMR spectroscopy. Plant Biotechnol J. 2022 10; 20(10):1928-1939. Opdensteinen P, Sperl LE, Mohamadi M, Kündgen-Redding N, Hagn F, Buyel JF. PMID: 35702941; PMCID: PMC9491462.
      View in: PubMed   Mentions: 1     Fields:    Translation:Cells
    6. Reducing water uptake into BY-2 cells by systematically optimizing the cultivation parameters increases product yields achieved by transient expression in plant cell packs. Biotechnol J. 2022 Oct; 17(10):e2200134. Opdensteinen P, Buyel JF. PMID: 35762355.
      View in: PubMed   Mentions: 1     Fields:    Translation:AnimalsCells
    7. Expression of Biofilm-Degrading Enzymes in Plants and Automated High-Throughput Activity Screening Using Experimental Bacillus subtilis Biofilms. Front Bioeng Biotechnol. 2021; 9:708150. Opdensteinen P, Dietz SJ, Gengenbach BB, Buyel JF. PMID: 34621728; PMCID: PMC8490750.
      View in: PubMed   Mentions: 1  
    8. Heterologous production of α-Carotene in Corynebacterium glutamicum using a multi-copy chromosomal integration method. Bioresour Technol. 2021 Dec; 341:125782. Li C, Swofford CA, Rückert C, Chatzivasileiou AO, Ou RW, Opdensteinen P, Luttermann T, Zhou K, Stephanopoulos G, Jones Prather KL, Zhong-Johnson EZL, Liang S, Zheng S, Lin Y, Sinskey AJ. PMID: 34419880.
      View in: PubMed   Mentions: 6     Fields:    Translation:HumansCells
    9. Precision analysis for the determination of steric mass action parameters using eight tobacco host cell proteins. J Chromatogr A. 2021 Aug 30; 1652:462379. Bernau CR, Jäpel RC, Hübbers JW, Nölting S, Opdensteinen P, Buyel JF. PMID: 34256268.
      View in: PubMed   Mentions: 1     Fields:    Translation:HumansCells
    10. A combined pH and temperature precipitation step facilitates the purification of tobacco-derived recombinant proteins that are sensitive to extremes of either parameter. Biotechnol J. 2021 Apr; 16(4):e2000340. Opdensteinen P, Lobanov A, Buyel JF. PMID: 33247609.
      View in: PubMed   Mentions: 4     Fields:    
    11. Robot Cookies - Plant Cell Packs as an Automated High-Throughput Screening Platform Based on Transient Expression. Front Bioeng Biotechnol. 2020; 8:393. Gengenbach BB, Opdensteinen P, Buyel JF. PMID: 32432097; PMCID: PMC7214789.
      View in: PubMed   Mentions: 13  
    12. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. J Vis Exp. 2019 08 16; (150). Knödler M, Rühl C, Opdensteinen P, Buyel JF. PMID: 31475984.
      View in: PubMed   Mentions: 3     Fields:    Translation:Animals
    13. Comparison of microbial and transient expression (tobacco plants and plant-cell packs) for the production and purification of the anticancer mistletoe lectin viscumin. Biotechnol Bioeng. 2019 09; 116(9):2236-2249. Gengenbach BB, Keil LL, Opdensteinen P, Müschen CR, Melmer G, Lentzen H, Bührmann J, Buyel JF. PMID: 31140580; PMCID: PMC6772165.
      View in: PubMed   Mentions: 20     Fields:    Translation:AnimalsCells
    14. A Combined Ultrafiltration/Diafiltration Step Facilitates the Purification of Cyanovirin-N From Transgenic Tobacco Extracts. Front Bioeng Biotechnol. 2018; 6:206. Opdensteinen P, Clodt JI, Müschen CR, Filiz V, Buyel JF. PMID: 30687700; PMCID: PMC6334625.
      View in: PubMed   Mentions: 10  
    15. A linear epitope coupled to DsRed provides an affinity ligand for the capture of monoclonal antibodies. J Chromatogr A. 2018 Oct 12; 1571:55-64. Rühl C, Knödler M, Opdensteinen P, Buyel JF. PMID: 30104060.
      View in: PubMed   Mentions: 5     Fields:    Translation:Animals
    16. Production of Functional Anti-Ebola Antibodies in Pichia pastoris. ACS Synth Biol. 2017 12 15; 6(12):2183-2190. Purcell O, Opdensteinen P, Chen W, Lowenhaupt K, Brown A, Hermann M, Cao J, Tenhaef N, Kallweit E, Kastilan R, Sinskey AJ, Perez-Pinera P, Buyel JF, Lu TK. PMID: 28786662.
      View in: PubMed   Mentions: 5     Fields:    Translation:HumansAnimalsCells
    17. Cellulose-based filter aids increase the capacity of depth filters during the downstream processing of plant-derived biopharmaceutical proteins. Biotechnol J. 2015 Apr; 10(4):584-91. Buyel JF, Opdensteinen P, Fischer R. PMID: 25611947.
      View in: PubMed   Mentions: 10     Fields:    Translation:Cells
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