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Steven Dowdy

TitleProfessor
InstitutionUniversity of California San Diego
DepartmentCellular and Molecular Medicine
Address9500 Gilman Drive #0686
La Jolla CA 92093
Phone858-534-7772
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    Collapse Overview 
    Collapse Overview
    Our lab is interested in the regulation of G1 cell cycle progression in cancer and determining the consequences of these genetic and epigenetic alterations and the mechanisms that activate the cell cycle machinery.

    Research Focus Areas



    Collapse Research 
    Collapse Research Activities and Funding
    Treating Adenovirus Conjunctivitis with Next-Gen siRNN RNAi Prodrugs
    NIH/NEI R21EY027507Mar 1, 2017 - Feb 28, 2019
    Role: Principal Investigator
    Treating Prostate Cancer with RNAi Prodrugs
    NIH/NCI R21CA195191Sep 1, 2015 - Aug 31, 2017
    Role: Principal Investigator
    Novel Cell Cycle Therapeutic Targets in Pancreatic Cancer
    NIH/NCI R21CA169849Mar 1, 2013 - Feb 28, 2015
    Role: Principal Investigator
    Transduction of Tumor Suppressor Proteins into Gliomas
    NIH/NCI R01CA096098Sep 5, 2001 - Jul 31, 2006
    Role: Principal Investigator
    Comprehensive Biology: Exploiting the Yeast Genome
    NIH P41RR011823Sep 30, 1996 - Jun 30, 2016
    Role: Co-Investigator

    Collapse ORNG Applications 
    Collapse Websites

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
    List All   |   Timeline
    1. Springer AD, Dowdy SF. GalNAc-siRNA Conjugates: Leading the Way for Delivery of RNAi Therapeutics. Nucleic Acid Ther. 2018 May 24. PMID: 29792572.
      View in: PubMed
    2. Dowdy SF, Levy M. RNA Therapeutics (Almost) Comes of Age: Targeting, Delivery and Endosomal Escape. Nucleic Acid Ther. 2018 May 21. PMID: 29782220.
      View in: PubMed
    3. Hagopian JC, Hamil AS, van den Berg A, Meade BR, Eguchi A, Palm-Apergi C, Dowdy SF. Induction of RNAi Responses by Short Left-Handed Hairpin RNAi Triggers. Nucleic Acid Ther. 2017 Oct; 27(5):260-271. PMID: 28933656.
      View in: PubMed
    4. Matsuda S, Hammaker D, Topolewski K, Briegel KJ, Boyle DL, Dowdy S, Wang W, Firestein GS. Regulation of the Cell Cycle and Inflammatory Arthritis by the Transcription Cofactor LBH Gene. J Immunol. 2017 10 01; 199(7):2316-2322. PMID: 28807995.
      View in: PubMed
    5. Yoshioka N, Dowdy SF. Enhanced generation of iPSCs from older adult human cells by a synthetic five-factor self-replicative RNA. PLoS One. 2017; 12(7):e0182018. PMID: 28750082.
      View in: PubMed
    6. Kolosenko I, Edsbäcker E, Björklund AC, Hamil AS, Goroshchuk O, Grandér D, Dowdy SF, Palm-Apergi C. RNAi prodrugs targeting Plk1 induce specific gene silencing in primary cells from pediatric T-acute lymphoblastic leukemia patients. J Control Release. 2017 09 10; 261:199-206. PMID: 28684168.
      View in: PubMed
    7. Dowdy SF. Overcoming cellular barriers for RNA therapeutics. Nat Biotechnol. 2017 Mar; 35(3):222-229. PMID: 28244992.
      View in: PubMed
    8. Lönn P, Kacsinta AD, Cui XS, Hamil AS, Kaulich M, Gogoi K, Dowdy SF. Enhancing Endosomal Escape for Intracellular Delivery of Macromolecular Biologic Therapeutics. Sci Rep. 2016 09 08; 6:32301. PMID: 27604151.
      View in: PubMed
    9. Meitinger F, Anzola JV, Kaulich M, Richardson A, Stender JD, Benner C, Glass CK, Dowdy SF, Desai A, Shiau AK, Oegema K. 53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration. J Cell Biol. 2016 07 18; 214(2):155-66. PMID: 27432897.
      View in: PubMed
    10. Amit I, Baker D, Barker R, Berger B, Bertozzi C, Bhatia S, Biffi A, Demichelis F, Doudna J, Dowdy SF, Endy D, Helmstaedter M, Junca H, June C, Kamb S, Khvorova A, Kim DH, Kim JS, Krishnan Y, Lakadamyali M, Lappalainen T, Lewin S, Liao J, Loman N, Lundberg E, Lynd L, Martin C, Mellman I, Miyawaki A, Mummery C, Nelson K, Paz J, Peralta-Yahya P, Picotti P, Polyak K, Prather K, Qin J, Quake S, Regev A, Rogers JA, Shetty R, Sommer M, Stevens M, Stolovitzky G, Takahashi M, Tang F, Teichmann S, Torres-Padilla ME, Tripathi L, Vemula P, Verdine G, Vollmer F, Wang J, Ying JY, Zhang F, Zhang T. Voices of biotech. Nat Biotechnol. 2016 Mar; 34(3):270-5. PMID: 26963549.
      View in: PubMed
    11. Hamil AS, Dowdy SF. Synthesis and Conjugation of Small Interfering Ribonucleic Neutral SiRNNs. Methods Mol Biol. 2016; 1364:1-9. PMID: 26472437.
      View in: PubMed
    12. Tobias IS, Kaulich M, Kim PK, Simon N, Jacinto E, Dowdy SF, King CC, Newton AC. Protein kinase C? exhibits constitutive phosphorylation and phosphatidylinositol-3,4,5-triphosphate-independent regulation. Biochem J. 2016 Feb 15; 473(4):509-23. PMID: 26635352; PMCID: PMC4888060.
    13. Kacsinta AD, Dowdy SF. Current views on inducing synthetic lethal RNAi responses in the treatment of cancer. Expert Opin Biol Ther. 2016; 16(2):161-72. PMID: 26630128.
      View in: PubMed
    14. Kaulich M, Dowdy SF. Combining CRISPR/Cas9 and rAAV Templates for Efficient Gene Editing. Nucleic Acid Ther. 2015 Dec; 25(6):287-96. PMID: 26540648; PMCID: PMC4657517 [Available on 12/01/16].
    15. Lönn P, Dowdy SF. Cationic PTD/CPP-mediated macromolecular delivery: charging into the cell. Expert Opin Drug Deliv. 2015; 12(10):1627-36. PMID: 25994800.
      View in: PubMed
    16. Shin D, Lönn P, Dowdy SF, Tor Y. Cellular activity of siRNA oligonucleotides containing synthetic isomorphic nucleoside surrogates. Chem Commun (Camb). 2015 Jan 31; 51(9):1662-5. PMID: 25500944; PMCID: PMC4298453.
    17. Kaulich M, Lee YJ, Lönn P, Springer AD, Meade BR, Dowdy SF. Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi. Nucleic Acids Res. 2015 Apr 20; 43(7):e45. PMID: 25586224; PMCID: PMC4402508.
    18. Meade BR, Gogoi K, Hamil AS, Palm-Apergi C, van den Berg A, Hagopian JC, Springer AD, Eguchi A, Kacsinta AD, Dowdy CF, Presente A, Lönn P, Kaulich M, Yoshioka N, Gros E, Cui XS, Dowdy SF. Efficient delivery of RNAi prodrugs containing reversible charge-neutralizing phosphotriester backbone modifications. Nat Biotechnol. 2014 Dec; 32(12):1256-61. PMID: 25402614; PMCID: PMC4378643.
    19. Lönn P, Dowdy SF. 14. From bench to bedside: cationic TAT peptide transduction domain enters cells by macropinocytosis (2005): Original research article: Cationic TAT peptide transduction domain enters cells by macropinocytosis, 2005. J Control Release. 2014 Sep 28; 190:56-8. PMID: 25356487.
      View in: PubMed
    20. Wu X, Zhang W, Font-Burgada J, Palmer T, Hamil AS, Biswas SK, Poidinger M, Borcherding N, Xie Q, Ellies LG, Lytle NK, Wu LW, Fox RG, Yang J, Dowdy SF, Reya T, Karin M. Ubiquitin-conjugating enzyme Ubc13 controls breast cancer metastasis through a TAK1-p38 MAP kinase cascade. Proc Natl Acad Sci U S A. 2014 Sep 23; 111(38):13870-5. PMID: 25189770; PMCID: PMC4183333.
    21. Narasimha AM, Kaulich M, Shapiro GS, Choi YJ, Sicinski P, Dowdy SF. Cyclin D activates the Rb tumor suppressor by mono-phosphorylation. Elife. 2014 Jun 04; 3. PMID: 24876129; PMCID: PMC4076869.
    22. Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, Chi NC, Fu XD, Yu BD, Dowdy SF. Efficient generation of human iPSCs by a synthetic self-replicative RNA. Cell Stem Cell. 2013 Aug 01; 13(2):246-54. PMID: 23910086; PMCID: PMC3845961.
    23. Kogiso T, Nagahara H, Otsuka M, Shiratori K, Dowdy SF. Transdifferentiation of human fibroblasts into hepatocyte-like cells by defined transcriptional factors. Hepatol Int. 2013 Jul; 7(3):937-44. PMID: 26201932.
      View in: PubMed
    24. Presente A, Dowdy SF. PTD/CPP peptide-mediated delivery of siRNAs. Curr Pharm Des. 2013; 19(16):2943-7. PMID: 23140460.
      View in: PubMed
    25. Wadia J, Eguchi A, Dowdy SF. DNA delivery into mammalian cells using bacteriophage ? displaying the TAT transduction domain. Cold Spring Harb Protoc. 2013 Jan 01; 2013(1). PMID: 23282641.
      View in: PubMed
    26. Keerthivasan G, Liu H, Gump JM, Dowdy SF, Wickrema A, Crispino JD. A novel role for survivin in erythroblast enucleation. Haematologica. 2012 Oct; 97(10):1471-9. PMID: 22491741; PMCID: PMC3487547.
    27. Palm-Apergi C, Lönn P, Dowdy SF. Do cell-penetrating peptides actually "penetrate" cellular membranes? Mol Ther. 2012 Apr; 20(4):695-7. PMID: 22472979; PMCID: PMC3322330.
    28. van den Berg A, Dowdy SF. Protein transduction domain delivery of therapeutic macromolecules. Curr Opin Biotechnol. 2011 Dec; 22(6):888-93. PMID: 21489777.
      View in: PubMed
    29. Palm-Apergi C, Eguchi A, Dowdy SF. PTD-DRBD siRNA delivery. Methods Mol Biol. 2011; 683:339-47. PMID: 21053141.
      View in: PubMed
    30. June RK, Gogoi K, Eguchi A, Cui XS, Dowdy SF. Synthesis of a pH-sensitive nitrilotriacetic linker to peptide transduction domains to enable intracellular delivery of histidine imidazole ring-containing macromolecules. J Am Chem Soc. 2010 Aug 11; 132(31):10680-2. PMID: 20681698; PMCID: PMC2915637.
    31. Pawar SA, Sarkar TR, Balamurugan K, Sharan S, Wang J, Zhang Y, Dowdy SF, Huang AM, Sterneck E. C/EBP{delta} targets cyclin D1 for proteasome-mediated degradation via induction of CDC27/APC3 expression. Proc Natl Acad Sci U S A. 2010 May 18; 107(20):9210-5. PMID: 20439707; PMCID: PMC2889124.
    32. Eguchi A, Dowdy SF. Efficient siRNA delivery by novel PTD-DRBD fusion proteins. Cell Cycle. 2010 Feb 01; 9(3):424-5. PMID: 20090414.
      View in: PubMed
    33. Michiue H, Eguchi A, Scadeng M, Dowdy SF. Induction of in vivo synthetic lethal RNAi responses to treat glioblastoma. Cancer Biol Ther. 2009 Dec; 8(23):2306-13. PMID: 19901546.
      View in: PubMed
    34. Meade BR, Dowdy SF. The road to therapeutic RNA interference (RNAi): Tackling the 800 pound siRNA delivery gorilla. Discov Med. 2009 Dec; 8(43):253-6. PMID: 20040280.
      View in: PubMed
    35. Gump JM, June RK, Dowdy SF. Revised role of glycosaminoglycans in TAT protein transduction domain-mediated cellular transduction. J Biol Chem. 2010 Jan 08; 285(2):1500-7. PMID: 19858185; PMCID: PMC2801275.
    36. Eguchi A, Dowdy SF. siRNA delivery using peptide transduction domains. Trends Pharmacol Sci. 2009 Jul; 30(7):341-5. PMID: 19545914.
      View in: PubMed
    37. Eguchi A, Meade BR, Chang YC, Fredrickson CT, Willert K, Puri N, Dowdy SF. Efficient siRNA delivery into primary cells by a peptide transduction domain-dsRNA binding domain fusion protein. Nat Biotechnol. 2009 Jun; 27(6):567-71. PMID: 19448630; PMCID: PMC2694965.
    38. Wagner MV, Smolka MB, de Bruin RA, Zhou H, Wittenberg C, Dowdy SF. Whi5 regulation by site specific CDK-phosphorylation in Saccharomyces cerevisiae. PLoS One. 2009; 4(1):e4300. PMID: 19172996; PMCID: PMC2627923.
    39. Cerchietti LC, Yang SN, Shaknovich R, Hatzi K, Polo JM, Chadburn A, Dowdy SF, Melnick A. A peptomimetic inhibitor of BCL6 with potent antilymphoma effects in vitro and in vivo. Blood. 2009 Apr 09; 113(15):3397-405. PMID: 18927431; PMCID: PMC2668844.
    40. Wadia JS, Schaller M, Williamson RA, Dowdy SF. Pathologic prion protein infects cells by lipid-raft dependent macropinocytosis. PLoS One. 2008; 3(10):e3314. PMID: 19390657; PMCID: PMC2671965.
    41. Xu Y, Liu S, Yu G, Chen J, Chen J, Xu X, Wu Y, Zhang A, Dowdy SF, Cheng G. Excision of selectable genes from transgenic goat cells by a protein transducible TAT-Cre recombinase. Gene. 2008 Aug 01; 419(1-2):70-4. PMID: 18547746.
      View in: PubMed
    42. Cerchietti LC, Polo JM, Da Silva GF, Farinha P, Shaknovich R, Gascoyne RD, Dowdy SF, Melnick A. Sequential transcription factor targeting for diffuse large B-cell lymphomas. Cancer Res. 2008 May 01; 68(9):3361-9. PMID: 18451163; PMCID: PMC2748725.
    43. Besson A, Dowdy SF, Roberts JM. CDK inhibitors: cell cycle regulators and beyond. Dev Cell. 2008 Feb; 14(2):159-69. PMID: 18267085.
      View in: PubMed
    44. Meade BR, Dowdy SF. Enhancing the cellular uptake of siRNA duplexes following noncovalent packaging with protein transduction domain peptides. Adv Drug Deliv Rev. 2008 Mar 01; 60(4-5):530-6. PMID: 18155315; PMCID: PMC2293332.
    45. Denicourt C, Saenz CC, Datnow B, Cui XS, Dowdy SF. Relocalized p27Kip1 tumor suppressor functions as a cytoplasmic metastatic oncogene in melanoma. Cancer Res. 2007 Oct 01; 67(19):9238-43. PMID: 17909030.
      View in: PubMed
    46. Gump JM, Dowdy SF. TAT transduction: the molecular mechanism and therapeutic prospects. Trends Mol Med. 2007 Oct; 13(10):443-8. PMID: 17913584.
      View in: PubMed
    47. Meade BR, Dowdy SF. Exogenous siRNA delivery using peptide transduction domains/cell penetrating peptides. Adv Drug Deliv Rev. 2007 Mar 30; 59(2-3):134-40. PMID: 17451840.
      View in: PubMed
    48. Muntean AG, Pang L, Poncz M, Dowdy SF, Blobel GA, Crispino JD. Cyclin D-Cdk4 is regulated by GATA-1 and required for megakaryocyte growth and polyploidization. Blood. 2007 Jun 15; 109(12):5199-207. PMID: 17317855; PMCID: PMC1890844.
    49. Haberichter T, Mädge B, Christopher RA, Yoshioka N, Dhiman A, Miller R, Gendelman R, Aksenov SV, Khalil IG, Dowdy SF. A systems biology dynamical model of mammalian G1 cell cycle progression. Mol Syst Biol. 2007; 3:84. PMID: 17299420; PMCID: PMC1828753.
    50. Dowdy SF. The anonymous American Idol manuscript reviewer. Cell. 2006 Nov 17; 127(4):662-3; author reply 664-5. PMID: 17110321.
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    51. Murriel CL, Dowdy SF. Influence of protein transduction domains on intracellular delivery of macromolecules. Expert Opin Drug Deliv. 2006 Nov; 3(6):739-46. PMID: 17076596.
      View in: PubMed
    52. Havens CG, Ho A, Yoshioka N, Dowdy SF. Regulation of late G1/S phase transition and APC Cdh1 by reactive oxygen species. Mol Cell Biol. 2006 Jun; 26(12):4701-11. PMID: 16738333; PMCID: PMC1489138.
    53. Cai SR, Xu G, Becker-Hapak M, Ma M, Dowdy SF, McLeod HL. The kinetics and tissue distribution of protein transduction in mice. Eur J Pharm Sci. 2006 Mar; 27(4):311-9. PMID: 16376528.
      View in: PubMed
    54. Snyder EL, Saenz CC, Denicourt C, Meade BR, Cui XS, Kaplan IM, Dowdy SF. Enhanced targeting and killing of tumor cells expressing the CXC chemokine receptor 4 by transducible anticancer peptides. Cancer Res. 2005 Dec 01; 65(23):10646-50. PMID: 16322205.
      View in: PubMed
    55. Kaplan IM, Wadia JS, Dowdy SF. Cationic TAT peptide transduction domain enters cells by macropinocytosis. J Control Release. 2005 Jan 20; 102(1):247-53. PMID: 15653149.
      View in: PubMed
    56. Snyder EL, Dowdy SF. Recent advances in the use of protein transduction domains for the delivery of peptides, proteins and nucleic acids in vivo. Expert Opin Drug Deliv. 2005 Jan; 2(1):43-51. PMID: 16296734.
      View in: PubMed
    57. Wadia JS, Dowdy SF. Transmembrane delivery of protein and peptide drugs by TAT-mediated transduction in the treatment of cancer. Adv Drug Deliv Rev. 2005 Feb 28; 57(4):579-96. PMID: 15722165.
      View in: PubMed
    58. Denicourt C, Dowdy SF. Medicine. Targeting apoptotic pathways in cancer cells. Science. 2004 Sep 03; 305(5689):1411-3. PMID: 15353788.
      View in: PubMed
    59. Denicourt C, Dowdy SF. Cip/Kip proteins: more than just CDKs inhibitors. Genes Dev. 2004 Apr 15; 18(8):851-5. PMID: 15107401.
      View in: PubMed
    60. Snyder EL, Dowdy SF. Cell penetrating peptides in drug delivery. Pharm Res. 2004 Mar; 21(3):389-93. PMID: 15070086.
      View in: PubMed
    61. Snyder EL, Meade BR, Saenz CC, Dowdy SF. Treatment of terminal peritoneal carcinomatosis by a transducible p53-activating peptide. PLoS Biol. 2004 Feb; 2(2):E36. PMID: 14966535; PMCID: PMC340944.
    62. Wadia JS, Stan RV, Dowdy SF. Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Nat Med. 2004 Mar; 10(3):310-5. PMID: 14770178.
      View in: PubMed
    63. Denicourt C, Dowdy SF. Another twist in the transforming growth factor beta-induced cell-cycle arrest chronicle. Proc Natl Acad Sci U S A. 2003 Dec 23; 100(26):15290-1. PMID: 14676328; PMCID: PMC307558.
    64. Yu BD, Becker-Hapak M, Snyder EL, Vooijs M, Denicourt C, Dowdy SF. Distinct and nonoverlapping roles for pRB and cyclin D:cyclin-dependent kinases 4/6 activity in melanocyte survival. Proc Natl Acad Sci U S A. 2003 Dec 09; 100(25):14881-6. PMID: 14630948; PMCID: PMC299840.
    65. Snyder EL, Meade BR, Dowdy SF. Anti-cancer protein transduction strategies: reconstitution of p27 tumor suppressor function. J Control Release. 2003 Aug 28; 91(1-2):45-51. PMID: 12932636.
      View in: PubMed
    66. Denicourt C, Dowdy SF. Protein transduction technology offers novel therapeutic approach for brain ischemia. Trends Pharmacol Sci. 2003 May; 24(5):216-8. PMID: 12767717.
      View in: PubMed
    67. Becker-Hapak M, Dowdy SF. Protein transduction: generation of full-length transducible proteins using the TAT system. Curr Protoc Cell Biol. 2003 May; Chapter 20:Unit 20.2. PMID: 18228426.
      View in: PubMed
    68. Wadia JS, Dowdy SF. Modulation of cellular function by TAT mediated transduction of full length proteins. Curr Protein Pept Sci. 2003 Apr; 4(2):97-104. PMID: 12678849.
      View in: PubMed
    69. Tanaka Y, Dowdy SF, Linehan DC, Eberlein TJ, Goedegebuure PS. Induction of antigen-specific CTL by recombinant HIV trans-activating fusion protein-pulsed human monocyte-derived dendritic cells. J Immunol. 2003 Feb 01; 170(3):1291-8. PMID: 12538688.
      View in: PubMed
    70. McAllister SS, Becker-Hapak M, Pintucci G, Pagano M, Dowdy SF. Novel p27(kip1) C-terminal scatter domain mediates Rac-dependent cell migration independent of cell cycle arrest functions. Mol Cell Biol. 2003 Jan; 23(1):216-28. PMID: 12482975; PMCID: PMC140659.
    71. Hsia CY, Cheng S, Owyang AM, Dowdy SF, Liou HC. c-Rel regulation of the cell cycle in primary mouse B lymphocytes. Int Immunol. 2002 Aug; 14(8):905-16. PMID: 12147627.
      View in: PubMed
    72. Ho A, Dowdy SF. Regulation of G(1) cell-cycle progression by oncogenes and tumor suppressor genes. Curr Opin Genet Dev. 2002 Feb; 12(1):47-52. PMID: 11790554.
      View in: PubMed
    73. Wadia JS, Dowdy SF. Protein transduction technology. Curr Opin Biotechnol. 2002 Feb; 13(1):52-6. PMID: 11849958.
      View in: PubMed