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Richard Klemke

TitleProfessor
InstitutionUniversity of California San Diego
DepartmentPathology
Address9500 Gilman Drive #0612
La Jolla CA 92093
Phone858-822-5610
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    Collapse Research 
    Collapse Research Activities and Funding
    Fingerprinting Invasive Membrane Protrusions to Discover Metastatic Signatures
    NIH/NCI R01CA182495Sep 1, 2014 - Aug 31, 2019
    Role: Principal Investigator
    Vascular communication in metastatic brain colonization
    NIH/NCI R01CA184594Apr 3, 2014 - Feb 28, 2019
    Role: Principal Investigator
    Pre-clinical development of a novel pancreatic cancer chemotherapeutic
    NIH/NCI R43CA180398Sep 1, 2013 - Aug 31, 2014
    Role: Principal Investigator
    Imagining Human Cancer Progression in a Novel Zebrafish Model
    NIH/NCI R01CA129231Aug 1, 2008 - May 31, 2013
    Role: Principal Investigator
    A Proteomics Research Resource for Integrative Biology
    NIH P41RR018522Sep 15, 2003 - Jun 30, 2013
    Role: Co-Investigator
    Mechanisms of Lamellipodia Growth and Retraction
    NIH/NIGMS R01GM068487Aug 1, 2003 - Jul 31, 2012
    Role: Principal Investigator
    Survival Mechanisms of Invasive Carcinoma Cells
    NIH/NCI R01CA097022Jul 1, 2002 - Feb 28, 2018
    Role: Principal Investigator
    Cell Migration Consortium
    NIH U54GM064346Sep 15, 2001 - Jul 31, 2014
    Role: Co-Investigator
    SIGNALING MECHANISMS OF CELL MOTILITY &TUMOR METASTASIS
    NIH/NCI R29CA078493Aug 1, 1998 - Jul 31, 2003
    Role: Principal Investigator
    Comprehensive Biology: Exploiting the Yeast Genome
    NIH P41RR011823Sep 30, 1996 - Jun 30, 2016
    Role: Co-Investigator
    MOLECULAR BASIS OF INTEGRIN MEDIATED CELL MIGRATION
    NIH/NCI F32CA067442Mar 31, 1996
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Wang H, Lapek J, Fujimura K, Strnadel J, Liu B, Gonzalez DJ, Zhang W, Watson F, Yu V, Liu C, Melo CM, Miller YI, Elliott KC, Cheresh DA, Klemke R. Pseudopodium-enriched atypical kinase 1 mediates angiogenesis by modulating GATA2-dependent VEGFR2 transcription. Cell Discov. 2018; 4:26. PMID: 29872538.
      View in: PubMed
    2. Liu C, Han T, Stachura DL, Wang H, Vaisman BL, Kim J, Klemke R, Remaley AT, Rana TM, Traver D, Miller YI. Lipoprotein lipase regulates hematopoietic stem progenitor cell maintenance through DHA supply. Nat Commun. 2018 Apr 03; 9(1):1310. PMID: 29615667.
      View in: PubMed
    3. Gilder AS, Natali L, Van Dyk DM, Zalfa C, Banki MA, Pizzo DP, Wang H, Klemke R, Mantuano E, Gonias SL. The Urokinase Receptor Induces a Mesenchymal Gene Expression Signature in Glioblastoma Cells and Promotes Tumor Cell Survival in Neurospheres. Sci Rep. 2018 Feb 14; 8(1):2982. PMID: 29445239.
      View in: PubMed
    4. Noriega-Guerra H, Cruz MC, Ribeiro PRL, Strnadel J, Wang H, Klemke R, Jaeger RG, Freitas VM. ADAMTS-1 disrupts HGF/c-MET signaling and HGF-stimulated cellular processes in fibrosarcoma. Exp Cell Res. 2018 Feb 15; 363(2):271-282. PMID: 29355494.
      View in: PubMed
    5. Fujimura K, Wang H, Watson F, Klemke R. KRAS Oncoprotein Expression Is Regulated by a Self-Governing eIF5A-PEAK1 Feed-Forward Regulatory Loop. Cancer Res. 2018 Mar 15; 78(6):1444-1456. PMID: 29321164.
      View in: PubMed
    6. Strnadel J, Choi S, Fujimura K, Wang H, Zhang W, Wyse M, Wright T, Gross E, Peinado C, Park HW, Bui J, Kelber J, Bouvet M, Guan KL, Klemke R. eIF5A-PEAK1 Signaling Regulates YAP1/TAZ Protein Expression and Pancreatic Cancer Cell Growth. Cancer Res. 2017 04 15; 77(8):1997-2007. PMID: 28381547.
      View in: PubMed
    7. Fujimura K, Choi S, Wyse M, Strnadel J, Wright T, Klemke R. Eukaryotic Translation Initiation Factor 5A (EIF5A) Regulates Pancreatic Cancer Metastasis by Modulating RhoA and Rho-associated Kinase (ROCK) Protein Expression Levels. J Biol Chem. 2015 Dec 11; 290(50):29907-19. PMID: 26483550; PMCID: PMC4706006 [Available on 12/11/16].
    8. Agajanian M, Campeau A, Hoover M, Hou A, Brambilla D, Kim SL, Klemke R, Kelber JA. PEAK1 Acts as a Molecular Switch to Regulate Context-Dependent TGFß Responses in Breast Cancer. PLoS One. 2015; 10(8):e0135748. PMID: 26267863; PMCID: PMC4533969.
    9. Fujimura K, Wright T, Strnadel J, Kaushal S, Metildi C, Lowy AM, Bouvet M, Kelber JA, Klemke R. A hypusine-eIF5A-PEAK1 switch regulates the pathogenesis of pancreatic cancer. Cancer Res. 2014 Nov 15; 74(22):6671-81. PMID: 25261239; PMCID: PMC4233190.
    10. Pasillas MP, Shields S, Reilly R, Strnadel J, Behl C, Park R, Yates JR, Klemke R, Gonias SL, Coppinger JA. Proteomic analysis reveals a role for Bcl2-associated athanogene 3 and major vault protein in resistance to apoptosis in senescent cells by regulating ERK1/2 activation. Mol Cell Proteomics. 2015 Jan; 14(1):1-14. PMID: 24997994; PMCID: PMC4288246.
    11. Spike BT, Kelber JA, Booker E, Kalathur M, Rodewald R, Lipianskaya J, La J, He M, Wright T, Klemke R, Wahl GM, Gray PC. CRIPTO/GRP78 signaling maintains fetal and adult mammary stem cells ex vivo. Stem Cell Reports. 2014 Apr 08; 2(4):427-39. PMID: 24749068; PMCID: PMC3986630.
    12. Choi S, Kelber J, Jiang X, Strnadel J, Fujimura K, Pasillas M, Coppinger J, Klemke R. Procedures for the biochemical enrichment and proteomic analysis of the cytoskeletome. Anal Biochem. 2014 Feb 01; 446:102-7. PMID: 24161902; PMCID: PMC3947189.
    13. Stoletov K, Strnadel J, Zardouzian E, Momiyama M, Park FD, Kelber JA, Pizzo DP, Hoffman R, VandenBerg SR, Klemke R. Role of connexins in metastatic breast cancer and melanoma brain colonization. J Cell Sci. 2013 Feb 15; 126(Pt 4):904-13. PMID: 23321642; PMCID: PMC3625812.
    14. Klemke R, Jiang X, Choi S, Kelber JA. Proteomic and biochemical methods to study the cytoskeletome. Methods Mol Biol. 2013; 1046:203-18. PMID: 23868590.
      View in: PubMed
    15. Bristow JM, Reno TA, Jo M, Gonias SL, Klemke R. Dynamic phosphorylation of tyrosine 665 in pseudopodium-enriched atypical kinase 1 (PEAK1) is essential for the regulation of cell migration and focal adhesion turnover. J Biol Chem. 2013 Jan 04; 288(1):123-31. PMID: 23105102; PMCID: PMC3537006.
    16. Klemke R. Trespassing cancer cells: 'fingerprinting' invasive protrusions reveals metastatic culprits. Curr Opin Cell Biol. 2012 Oct; 24(5):662-9. PMID: 22980730; PMCID: PMC3489010.
    17. Soman P, Kelber JA, Lee JW, Wright TN, Vecchio KS, Klemke R, Chen S. Cancer cell migration within 3D layer-by-layer microfabricated photocrosslinked PEG scaffolds with tunable stiffness. Biomaterials. 2012 Oct; 33(29):7064-70. PMID: 22809641; PMCID: PMC3420339.
    18. Kelber JA, Reno T, Kaushal S, Metildi C, Wright T, Stoletov K, Weems JM, Park FD, Mose E, Wang Y, Hoffman RM, Lowy AM, Bouvet M, Klemke R. KRas induces a Src/PEAK1/ErbB2 kinase amplification loop that drives metastatic growth and therapy resistance in pancreatic cancer. Cancer Res. 2012 May 15; 72(10):2554-64. PMID: 22589274; PMCID: PMC3366503.
    19. Wang Y, Klemke R. Proteomics method for identification of pseudopodium phosphotyrosine proteins. Methods Mol Biol. 2012; 757:349-65. PMID: 21909922.
      View in: PubMed
    20. Wang Y, Yang F, Gritsenko MA, Wang Y, Clauss T, Liu T, Shen Y, Monroe ME, Lopez-Ferrer D, Reno T, Moore RJ, Klemke R, Camp DG, Smith RD. Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells. Proteomics. 2011 May; 11(10):2019-26. PMID: 21500348; PMCID: PMC3120047.
    21. Wang Y, Yang F, Fu Y, Huang X, Wang W, Jiang X, Gritsenko MA, Zhao R, Monore ME, Pertz OC, Purvine SO, Orton DJ, Jacobs JM, Camp DG, Smith RD, Klemke R. Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem. 2011 May 20; 286(20):18190-201. PMID: 21454597; PMCID: PMC3093891.
    22. Jo M, Eastman BM, Webb DL, Stoletov K, Klemke R, Gonias SL. Cell signaling by urokinase-type plasminogen activator receptor induces stem cell-like properties in breast cancer cells. Cancer Res. 2010 Nov 01; 70(21):8948-58. PMID: 20940399; PMCID: PMC2970644.
    23. Jin H, Garmy-Susini B, Avraamides CJ, Stoletov K, Klemke R, Varner JA. A PKA-Csk-pp60Src signaling pathway regulates the switch between endothelial cell invasion and cell-cell adhesion during vascular sprouting. Blood. 2010 Dec 16; 116(25):5773-83. PMID: 20826718; PMCID: PMC3031419.
    24. Kelber JA, Klemke R. PEAK1, a novel kinase target in the fight against cancer. Oncotarget. 2010 Jul; 1(3):219-23. PMID: 21301050; PMCID: PMC3057678.
    25. Stoletov K, Kato H, Zardouzian E, Kelber J, Yang J, Shattil S, Klemke R. Visualizing extravasation dynamics of metastatic tumor cells. J Cell Sci. 2010 Jul 01; 123(Pt 13):2332-41. PMID: 20530574; PMCID: PMC2886748.
    26. Wang Y, Kelber JA, Tran Cao HS, Cantin GT, Lin R, Wang W, Kaushal S, Bristow JM, Edgington TS, Hoffman RM, Bouvet M, Yates JR, Klemke R. Pseudopodium-enriched atypical kinase 1 regulates the cytoskeleton and cancer progression [corrected]. Proc Natl Acad Sci U S A. 2010 Jun 15; 107(24):10920-5. PMID: 20534451; PMCID: PMC2890752.
    27. Sampathkumar P, Ozyurt SA, Miller SA, Bain KT, Rutter ME, Gheyi T, Abrams B, Wang Y, Atwell S, Luz JG, Thompson DA, Wasserman SR, Emtage JS, Park EC, Rongo C, Jin Y, Klemke R, Sauder JM, Burley SK. Structures of PHR domains from Mus musculus Phr1 (Mycbp2) explain the loss-of-function mutation (Gly1092-->Glu) of the C. elegans ortholog RPM-1. J Mol Biol. 2010 Apr 09; 397(4):883-92. PMID: 20156452; PMCID: PMC2881670.
    28. Murphy EA, Shields DJ, Stoletov K, Dneprovskaia E, McElroy M, Greenberg JI, Lindquist J, Acevedo LM, Anand S, Majeti BK, Tsigelny I, Saldanha A, Walsh B, Hoffman RM, Bouvet M, Klemke R, Vogt PK, Arnold L, Wrasidlo W, Cheresh DA. Disruption of angiogenesis and tumor growth with an orally active drug that stabilizes the inactive state of PDGFRbeta/B-RAF. Proc Natl Acad Sci U S A. 2010 Mar 02; 107(9):4299-304. PMID: 20154271; PMCID: PMC2840076.
    29. Green CE, Liu T, Montel V, Hsiao G, Lester RD, Subramaniam S, Gonias SL, Klemke R. Chemoattractant signaling between tumor cells and macrophages regulates cancer cell migration, metastasis and neovascularization. PLoS One. 2009 Aug 21; 4(8):e6713. PMID: 19696929; PMCID: PMC2725301.
    30. Stoletov K, Fang L, Choi SH, Hartvigsen K, Hansen LF, Hall C, Pattison J, Juliano J, Miller ER, Almazan F, Crosier P, Witztum JL, Klemke R, Miller YI. Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish. Circ Res. 2009 Apr 24; 104(8):952-60. PMID: 19265037; PMCID: PMC2834250.
    31. Ding SJ, Wang Y, Jacobs JM, Qian WJ, Yang F, Tolmachev AV, Du X, Wang W, Moore RJ, Monroe ME, Purvine SO, Waters K, Heibeck TH, Adkins JN, Camp DG, Klemke R, Smith RD. Quantitative phosphoproteome analysis of lysophosphatidic acid induced chemotaxis applying dual-step (18)O labeling coupled with immobilized metal-ion affinity chromatography. J Proteome Res. 2008 Oct; 7(10):4215-24. PMID: 18785766; PMCID: PMC2791456.
    32. Wrasidlo W, Mielgo A, Torres VA, Barbero S, Stoletov K, Suyama TL, Klemke R, Gerwick WH, Carson DA, Stupack DG. The marine lipopeptide somocystinamide A triggers apoptosis via caspase 8. Proc Natl Acad Sci U S A. 2008 Feb 19; 105(7):2313-8. PMID: 18268346; PMCID: PMC2268133.
    33. Pertz OC, Wang Y, Yang F, Wang W, Gay LJ, Gristenko MA, Clauss TR, Anderson DJ, Liu T, Auberry KJ, Camp DG, Smith RD, Klemke R. Spatial mapping of the neurite and soma proteomes reveals a functional Cdc42/Rac regulatory network. Proc Natl Acad Sci U S A. 2008 Feb 12; 105(6):1931-6. PMID: 18245386; PMCID: PMC2542865.
    34. Stoletov K, Montel V, Lester RD, Gonias SL, Klemke R. High-resolution imaging of the dynamic tumor cell vascular interface in transparent zebrafish. Proc Natl Acad Sci U S A. 2007 Oct 30; 104(44):17406-11. PMID: 17954920; PMCID: PMC2077269.
    35. Wang Y, Klemke R. PhosphoBlast, a computational tool for comparing phosphoprotein signatures among large datasets. Mol Cell Proteomics. 2008 Jan; 7(1):145-62. PMID: 17934212.
      View in: PubMed
    36. Wang Y, Ding SJ, Wang W, Yang F, Jacobs JM, Camp D, Smith RD, Klemke R. Methods for pseudopodia purification and proteomic analysis. Sci STKE. 2007 Aug 21; 2007(400):pl4. PMID: 17712138.
      View in: PubMed
    37. Wang Y, Ding SJ, Wang W, Jacobs JM, Qian WJ, Moore RJ, Yang F, Camp DG, Smith RD, Klemke R. Profiling signaling polarity in chemotactic cells. Proc Natl Acad Sci U S A. 2007 May 15; 104(20):8328-33. PMID: 17494752; PMCID: PMC1895949.
    38. Wang Y, Klemke R. Biochemical purification of pseudopodia from migratory cells. Methods Mol Biol. 2007; 370:55-66. PMID: 17416987.
      View in: PubMed
    39. Wang Y, Hanley R, Klemke R. Computational methods for comparison of large genomic and proteomic datasets reveal protein markers of metastatic cancer. J Proteome Res. 2006 Apr; 5(4):907-15. PMID: 16602698.
      View in: PubMed
    40. Pertz O, Hodgson L, Klemke R, Hahn KM. Spatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006 Apr 20; 440(7087):1069-72. PMID: 16547516.
      View in: PubMed
    41. Holcomb M, Rufini A, Barilà D, Klemke R. Deregulation of proteasome function induces Abl-mediated cell death by uncoupling p130CAS and c-CrkII. J Biol Chem. 2006 Feb 03; 281(5):2430-40. PMID: 16267043.
      View in: PubMed
    42. Chodniewicz D, Klemke R. Guiding cell migration through directed extension and stabilization of pseudopodia. Exp Cell Res. 2004 Nov 15; 301(1):31-7. PMID: 15501442.
      View in: PubMed
    43. Wozniak MA, Kwong L, Chodniewicz D, Klemke R, Keely PJ. R-Ras controls membrane protrusion and cell migration through the spatial regulation of Rac and Rho. Mol Biol Cell. 2005 Jan; 16(1):84-96. PMID: 15525681; PMCID: PMC539154.
    44. Chodniewicz D, Klemke R. Regulation of integrin-mediated cellular responses through assembly of a CAS/Crk scaffold. Biochim Biophys Acta. 2004 Jul 05; 1692(2-3):63-76. PMID: 15246680.
      View in: PubMed
    45. Lin YH, Park ZY, Lin D, Brahmbhatt AA, Rio MC, Yates JR, Klemke R. Regulation of cell migration and survival by focal adhesion targeting of Lasp-1. J Cell Biol. 2004 May 10; 165(3):421-32. PMID: 15138294; PMCID: PMC2172195.
    46. Kain KH, Gooch S, Klemke R. Cytoplasmic c-Abl provides a molecular 'Rheostat' controlling carcinoma cell survival and invasion. Oncogene. 2003 Sep 04; 22(38):6071-80. PMID: 12955086.
      View in: PubMed
    47. Smit M, Leng J, Klemke R. Assay for neurite outgrowth quantification. Biotechniques. 2003 Aug; 35(2):254-6. PMID: 12951763.
      View in: PubMed
    48. Brahmbhatt AA, Klemke R. ERK and RhoA differentially regulate pseudopodia growth and retraction during chemotaxis. J Biol Chem. 2003 Apr 11; 278(15):13016-25. PMID: 12571246.
      View in: PubMed
    49. Cho SY, Klemke R. Purification of pseudopodia from polarized cells reveals redistribution and activation of Rac through assembly of a CAS/Crk scaffold. J Cell Biol. 2002 Feb 18; 156(4):725-36. PMID: 11839772; PMCID: PMC2174083.
    50. Ting AY, Kain KH, Klemke R, Tsien RY. Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells. Proc Natl Acad Sci U S A. 2001 Dec 18; 98(26):15003-8. PMID: 11752449; PMCID: PMC64973.
    51. Kain KH, Klemke R. Inhibition of cell migration by Abl family tyrosine kinases through uncoupling of Crk-CAS complexes. J Biol Chem. 2001 May 11; 276(19):16185-92. PMID: 11279004.
      View in: PubMed
    52. Cho SY, Klemke R. Extracellular-regulated kinase activation and CAS/Crk coupling regulate cell migration and suppress apoptosis during invasion of the extracellular matrix. J Cell Biol. 2000 Apr 03; 149(1):223-36. PMID: 10747099; PMCID: PMC2175095.
    53. Spencer KS, Graus-Porta D, Leng J, Hynes NE, Klemke R. ErbB2 is necessary for induction of carcinoma cell invasion by ErbB family receptor tyrosine kinases. J Cell Biol. 2000 Jan 24; 148(2):385-97. PMID: 10648571; PMCID: PMC2174273.
    54. Stupack DG, Cho SY, Klemke R. Molecular signaling mechanisms of cell migration and invasion. Immunol Res. 2000; 21(2-3):83-8. PMID: 10852105.
      View in: PubMed
    55. Leng J, Klemke R, Reddy AC, Cheresh DA. Potentiation of cell migration by adhesion-dependent cooperative signals from the GTPase Rac and Raf kinase. J Biol Chem. 1999 Dec 31; 274(53):37855-61. PMID: 10608850.
      View in: PubMed
    56. Cheresh DA, Leng J, Klemke R. Regulation of cell contraction and membrane ruffling by distinct signals in migratory cells. J Cell Biol. 1999 Sep 06; 146(5):1107-16. PMID: 10477763; PMCID: PMC2169492.
    57. Voice JK, Klemke R, Le A, Jackson JH. Four human ras homologs differ in their abilities to activate Raf-1, induce transformation, and stimulate cell motility. J Biol Chem. 1999 Jun 11; 274(24):17164-70. PMID: 10358073.
      View in: PubMed
    58. Klemke R, Leng J, Molander R, Brooks PC, Vuori K, Cheresh DA. CAS/Crk coupling serves as a "molecular switch" for induction of cell migration. J Cell Biol. 1998 Feb 23; 140(4):961-72. PMID: 9472046; PMCID: PMC2141747.
    59. Klemke R, Cai S, Giannini AL, Gallagher PJ, de Lanerolle P, Cheresh DA. Regulation of cell motility by mitogen-activated protein kinase. J Cell Biol. 1997 Apr 21; 137(2):481-92. PMID: 9128257; PMCID: PMC2139771.
    60. Brooks PC, Klemke R, Schon S, Lewis JM, Schwartz MA, Cheresh DA. Insulin-like growth factor receptor cooperates with integrin alpha v beta 5 to promote tumor cell dissemination in vivo. J Clin Invest. 1997 Mar 15; 99(6):1390-8. PMID: 9077549; PMCID: PMC507955.
    61. Klemke R, Yebra M, Bayna EM, Cheresh DA. Receptor tyrosine kinase signaling required for integrin alpha v beta 5-directed cell motility but not adhesion on vitronectin. J Cell Biol. 1994 Nov; 127(3):859-66. PMID: 7525598; PMCID: PMC2120222.
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