Marilyn Farquhar

TitleRecall Faculty
InstitutionUniversity of California San Diego
DepartmentCellular and Molecular Medicine
Address9500 Gilman Drive #0651
La Jolla CA 92093
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    Collapse Overview 
    Collapse Overview
    Our lab uses cell biology, bioinformatics and proteomics approaches to define signaling networks and to study the spatial regulation of signaling mediated by protein trafficking and targeting along the exocytic and endocytic pathways. Emphasis is on trafficking and signaling events that regulate growth factor receptors and G protein coupled receptors controlling cell growth and proliferation. A second area of interest centers on defining the signaling and trafficking events regulating glomerular filtration and protein absorption by the kidney and on pinpointing the molecular defects in these processes that occur in kidney diseases associated with proteinuria.

    Research Focus Area:

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    Collapse Research Activities and Funding
    JEOL JEM-1400Plus Transmission Electron Microscope
    NIH S10OD023527May 1, 2017 - Apr 30, 2018
    Role: Principal Investigator
    FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope
    NIH/NCRR S10RR027744Jan 28, 2010 - Jan 27, 2011
    Role: Principal Investigator
    Spatial Regulation of RGS and G Protein Signaling
    NIH/NCI R01CA100768Apr 17, 2003 - Jan 31, 2020
    Role: Principal Investigator
    Discovery and Development of Therapeutic Genes for CHF
    NIH P01HL066941Sep 29, 2000 - Jun 30, 2018
    Role: Co-Investigator
    Cancer Biology, Informatics & Omics (CBIO) Training Program
    NIH/NCI T32CA067754Sep 25, 1995 - Jun 30, 2022
    Role: Principal Investigator
    NIH/NCI P01CA058689Aug 25, 1993 - Mar 31, 2004
    Role: Principal Investigator
    Membrane Traffic in Normal and Transformed Cells
    NIH/NCI P01CA046128May 1, 1988 - Dec 31, 2007
    Role: Principal Investigator
    Glomerular Capillaries-- Normal and Pathologic
    NIH/NIDDK R37DK017724May 1, 1979 - May 31, 2017
    Role: Principal Investigator
    Glomerular Capillaries-- Normal and Pathologic
    NIH/NIDDK R01DK017724May 1, 1979 - Apr 30, 2009
    Role: Principal Investigator
    NIH/NIADDK R01AM017724May 1, 1979 - Apr 30, 1989
    Role: Principal Investigator
    Hormone Secretion and Processing in Pituitary Cells
    NIH/NIDDK R01DK017780Jan 1, 1978 - Dec 31, 2011
    Role: Principal Investigator
    NIH/NIDDK R37DK017780Jan 1, 1978 - Jun 30, 1996
    Role: Principal Investigator
    NIH/NIADDK R01AM017780Jan 1, 1978 - Dec 31, 1987
    Role: Principal Investigator

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    Collapse Bibliographic 
    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Aznar N, Patel A, Rohena CC, Dunkel Y, Joosen LP, Taupin V, Kufareva I, Farquhar MG, Ghosh P. AMP-activated protein kinase fortifies epithelial tight junctions during energetic stress via its effector GIV/Girdin. Elife. 2016 11 04; 5. PMID: 27813479.
      View in: PubMed
    2. Gupta V, Bhandari D, Leyme A, Aznar N, Midde KK, Lo IC, Ear J, Niesman I, López-Sánchez I, Blanco-Canosa JB, von Zastrow M, Garcia-Marcos M, Farquhar MG, Ghosh P. GIV/Girdin activates Gai and inhibits Gas via the same motif. Proc Natl Acad Sci U S A. 2016 09 27; 113(39):E5721-30. PMID: 27621449.
      View in: PubMed
    3. Bhandari D, Lopez-Sanchez I, To A, Lo IC, Aznar N, Leyme A, Gupta V, Niesman I, Maddox AL, Garcia-Marcos M, Farquhar MG, Ghosh P. Cyclin-dependent kinase 5 activates guanine nucleotide exchange factor GIV/Girdin to orchestrate migration-proliferation dichotomy. Proc Natl Acad Sci U S A. 2015 Sep 01; 112(35):E4874-83. PMID: 26286990; PMCID: PMC4568279.
    4. Lo IC, Gupta V, Midde KK, Taupin V, Lopez-Sanchez I, Kufareva I, Abagyan R, Randazzo PA, Farquhar MG, Ghosh P. Activation of Gai at the Golgi by GIV/Girdin imposes finiteness in Arf1 signaling. Dev Cell. 2015 Apr 20; 33(2):189-203. PMID: 25865347; PMCID: PMC4415880.
    5. Garcia-Marcos M, Ghosh P, Farquhar MG. GIV/Girdin transmits signals from multiple receptors by triggering trimeric G protein activation. J Biol Chem. 2015 Mar 13; 290(11):6697-704. PMID: 25605737; PMCID: PMC4358093.
    6. Wang H, Misaki T, Taupin V, Eguchi A, Ghosh P, Farquhar MG. GIV/girdin links vascular endothelial growth factor signaling to Akt survival signaling in podocytes independent of nephrin. J Am Soc Nephrol. 2015 Feb; 26(2):314-27. PMID: 25012178; PMCID: PMC4310647.
    7. Declèves AE, Zolkipli Z, Satriano J, Wang L, Nakayama T, Rogac M, Le TP, Nortier JL, Farquhar MG, Naviaux RK, Sharma K. Regulation of lipid accumulation by AMP-activated kinase [corrected] in high fat diet-induced kidney injury. Kidney Int. 2014 Mar; 85(3):611-23. PMID: 24304883.
      View in: PubMed
    8. Farquhar M. Marilyn Farquhar: from the beginning. Interview by Sedwick Caitlin. J Cell Biol. 2013 Nov 25; 203(4):554-5. PMID: 24385482; PMCID: PMC3840935.
    9. Shah M, Baterina OY, Taupin V, Farquhar MG. ARH directs megalin to the endocytic recycling compartment to regulate its proteolysis and gene expression. J Cell Biol. 2013 Jul 08; 202(1):113-27. PMID: 23836931; PMCID: PMC3704979.
    10. López-Sánchez I, Garcia-Marcos M, Mittal Y, Aznar N, Farquhar MG, Ghosh P. Protein kinase C-theta (PKC?) phosphorylates and inhibits the guanine exchange factor, GIV/Girdin. Proc Natl Acad Sci U S A. 2013 Apr 02; 110(14):5510-5. PMID: 23509302; PMCID: PMC3619294.
    11. Varsano T, Taupin V, Guo L, Baterina OY, Farquhar MG. The PDZ protein GIPC regulates trafficking of the LPA1 receptor from APPL signaling endosomes and attenuates the cell's response to LPA. PLoS One. 2012; 7(11):e49227. PMID: 23145131; PMCID: PMC3493537.
    12. Beas AO, Taupin V, Teodorof C, Nguyen LT, Garcia-Marcos M, Farquhar MG. Gas promotes EEA1 endosome maturation and shuts down proliferative signaling through interaction with GIV (Girdin). Mol Biol Cell. 2012 Dec; 23(23):4623-34. PMID: 23051738; PMCID: PMC3510023.
    13. Farquhar MG. A man for all seasons: reflections on the life and legacy of George Palade. Annu Rev Cell Dev Biol. 2012; 28:1-28. PMID: 22831641.
      View in: PubMed
    14. Wasik AA, Polianskyte-Prause Z, Dong MQ, Shaw AS, Yates JR, Farquhar MG, Lehtonen S. Septin 7 forms a complex with CD2AP and nephrin and regulates glucose transporter trafficking. Mol Biol Cell. 2012 Sep; 23(17):3370-9. PMID: 22809625; PMCID: PMC3431928.
    15. Dvir H, Shah M, Girardi E, Guo L, Farquhar MG, Zajonc DM. Atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain in complex with the LDL-receptor tail. Proc Natl Acad Sci U S A. 2012 May 01; 109(18):6916-21. PMID: 22509010; PMCID: PMC3344954.
    16. Garcia-Marcos M, Kietrsunthorn PS, Pavlova Y, Adia MA, Ghosh P, Farquhar MG. Functional characterization of the guanine nucleotide exchange factor (GEF) motif of GIV protein reveals a threshold effect in signaling. Proc Natl Acad Sci U S A. 2012 Feb 07; 109(6):1961-6. PMID: 22308453; PMCID: PMC3277514.
    17. Gallegos TF, Kouznetsova V, Kudlicka K, Sweeney DE, Bush KT, Willert K, Farquhar MG, Nigam SK. A protein kinase A and Wnt-dependent network regulating an intermediate stage in epithelial tubulogenesis during kidney development. Dev Biol. 2012 Apr 01; 364(1):11-21. PMID: 22290330; PMCID: PMC3310225.
    18. Fukasawa H, Obayashi H, Schmieder S, Lee J, Ghosh P, Farquhar MG. Phosphorylation of podocalyxin (Ser415) Prevents RhoA and ezrin activation and disrupts its interaction with the actin cytoskeleton. Am J Pathol. 2011 Nov; 179(5):2254-65. PMID: 21945805; PMCID: PMC3204015.
    19. Garcia-Marcos M, Kietrsunthorn PS, Wang H, Ghosh P, Farquhar MG. G Protein binding sites on Calnuc (nucleobindin 1) and NUCB2 (nucleobindin 2) define a new class of G(alpha)i-regulatory motifs. J Biol Chem. 2011 Aug 12; 286(32):28138-49. PMID: 21653697; PMCID: PMC3151059.
    20. Ghosh P, Garcia-Marcos M, Farquhar MG. GIV/Girdin is a rheostat that fine-tunes growth factor signals during tumor progression. Cell Adh Migr. 2011 May-Jun; 5(3):237-48. PMID: 21546796; PMCID: PMC3210208.
    21. Garcia-Marcos M, Ear J, Farquhar MG, Ghosh P. A GDI (AGS3) and a GEF (GIV) regulate autophagy by balancing G protein activity and growth factor signals. Mol Biol Cell. 2011 Mar 01; 22(5):673-86. PMID: 21209316; PMCID: PMC3046063.
    22. Schmid SL, Farquhar MG. The Palade symposium: celebrating cell biology at its best. Mol Biol Cell. 2010 Jul 15; 21(14):2367-70. PMID: 20505070; PMCID: PMC2903666.
    23. Ghosh P, Beas AO, Bornheimer SJ, Garcia-Marcos M, Forry EP, Johannson C, Ear J, Jung BH, Cabrera B, Carethers JM, Farquhar MG. A G{alpha}i-GIV molecular complex binds epidermal growth factor receptor and determines whether cells migrate or proliferate. Mol Biol Cell. 2010 Jul 01; 21(13):2338-54. PMID: 20462955; PMCID: PMC2893996.
    24. Garcia-Marcos M, Ghosh P, Ear J, Farquhar MG. A structural determinant that renders G alpha(i) sensitive to activation by GIV/girdin is required to promote cell migration. J Biol Chem. 2010 Apr 23; 285(17):12765-77. PMID: 20157114; PMCID: PMC2857069.
    25. Dippold HC, Ng MM, Farber-Katz SE, Lee SK, Kerr ML, Peterman MC, Sim R, Wiharto PA, Galbraith KA, Madhavarapu S, Fuchs GJ, Meerloo T, Farquhar MG, Zhou H, Field SJ. GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote budding. Cell. 2009 Oct 16; 139(2):337-51. PMID: 19837035; PMCID: PMC2779841.
    26. Fukasawa H, Bornheimer S, Kudlicka K, Farquhar MG. Slit diaphragms contain tight junction proteins. J Am Soc Nephrol. 2009 Jul; 20(7):1491-503. PMID: 19478094; PMCID: PMC2709684.
    27. Lin P, Fischer T, Lavoie C, Huang H, Farquhar MG. Calnuc plays a role in dynamic distribution of Galphai but not Gbeta subunits and modulates ACTH secretion in AtT-20 neuroendocrine secretory cells. Mol Neurodegener. 2009 Mar 25; 4:15. PMID: 19320978; PMCID: PMC2670299.
    28. Garcia-Marcos M, Ghosh P, Farquhar MG. GIV is a nonreceptor GEF for G alpha i with a unique motif that regulates Akt signaling. Proc Natl Acad Sci U S A. 2009 Mar 03; 106(9):3178-83. PMID: 19211784; PMCID: PMC2651282.
    29. Tang T, Zheng B, Chen SH, Murphy AN, Kudlicka K, Zhou H, Farquhar MG. hNOA1 interacts with complex I and DAP3 and regulates mitochondrial respiration and apoptosis. J Biol Chem. 2009 Feb 20; 284(8):5414-24. PMID: 19103604; PMCID: PMC2643507.
    30. Ghosh P, Garcia-Marcos M, Bornheimer SJ, Farquhar MG. Activation of Galphai3 triggers cell migration via regulation of GIV. J Cell Biol. 2008 Jul 28; 182(2):381-93. PMID: 18663145; PMCID: PMC2483528.
    31. Lehtonen S, Shah M, Nielsen R, Iino N, Ryan JJ, Zhou H, Farquhar MG. The endocytic adaptor protein ARH associates with motor and centrosomal proteins and is involved in centrosome assembly and cytokinesis. Mol Biol Cell. 2008 Jul; 19(7):2949-61. PMID: 18417616; PMCID: PMC2441659.
    32. Green RS, Stone EL, Tenno M, Lehtonen E, Farquhar MG, Marth JD. Mammalian N-glycan branching protects against innate immune self-recognition and inflammation in autoimmune disease pathogenesis. Immunity. 2007 Aug; 27(2):308-20. PMID: 17681821.
      View in: PubMed
    33. Chen Y, Lin P, Qiu S, Peng XX, Looi K, Farquhar MG, Zhang JY. Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection. Int J Oncol. 2007 May; 30(5):1137-44. PMID: 17390015.
      View in: PubMed
    34. Patel HH, Zhang S, Murray F, Suda RY, Head BP, Yokoyama U, Swaney JS, Niesman IR, Schermuly RT, Pullamsetti SS, Thistlethwaite PA, Miyanohara A, Farquhar MG, Yuan JX, Insel PA. Increased smooth muscle cell expression of caveolin-1 and caveolae contribute to the pathophysiology of idiopathic pulmonary arterial hypertension. FASEB J. 2007 Sep; 21(11):2970-9. PMID: 17470567.
      View in: PubMed
    35. Lin P, Li F, Zhang YW, Huang H, Tong G, Farquhar MG, Xu H. Calnuc binds to Alzheimer's beta-amyloid precursor protein and affects its biogenesis. J Neurochem. 2007 Mar; 100(6):1505-14. PMID: 17348862.
      View in: PubMed
    36. Zheng B, Tang T, Tang N, Kudlicka K, Ohtsubo K, Ma P, Marth JD, Farquhar MG, Lehtonen E. Essential role of RGS-PX1/sorting nexin 13 in mouse development and regulation of endocytosis dynamics. Proc Natl Acad Sci U S A. 2006 Nov 07; 103(45):16776-81. PMID: 17077144; PMCID: PMC1636531.
    37. Varsano T, Dong MQ, Niesman I, Gacula H, Lou X, Ma T, Testa JR, Yates JR, Farquhar MG. GIPC is recruited by APPL to peripheral TrkA endosomes and regulates TrkA trafficking and signaling. Mol Cell Biol. 2006 Dec; 26(23):8942-52. PMID: 17015470; PMCID: PMC1636811.
    38. Farquhar MG. The glomerular basement membrane: not gone, just forgotten. J Clin Invest. 2006 Aug; 116(8):2090-3. PMID: 16886057; PMCID: PMC1523392.
    39. Head BP, Patel HH, Roth DM, Murray F, Swaney JS, Niesman IR, Farquhar MG, Insel PA. Microtubules and actin microfilaments regulate lipid raft/caveolae localization of adenylyl cyclase signaling components. J Biol Chem. 2006 Sep 08; 281(36):26391-9. PMID: 16818493.
      View in: PubMed
    40. Lehtonen S, Ryan JJ, Kudlicka K, Iino N, Zhou H, Farquhar MG. Cell junction-associated proteins IQGAP1, MAGI-2, CASK, spectrins, and alpha-actinin are components of the nephrin multiprotein complex. Proc Natl Acad Sci U S A. 2005 Jul 12; 102(28):9814-9. PMID: 15994232; PMCID: PMC1175008.
    41. Head BP, Patel HH, Roth DM, Lai NC, Niesman IR, Farquhar MG, Insel PA. G-protein-coupled receptor signaling components localize in both sarcolemmal and intracellular caveolin-3-associated microdomains in adult cardiac myocytes. J Biol Chem. 2005 Sep 02; 280(35):31036-44. PMID: 15961389.
      View in: PubMed
    42. Le-Niculescu H, Niesman I, Fischer T, DeVries L, Farquhar MG. Identification and characterization of GIV, a novel Galpha i/s-interacting protein found on COPI, endoplasmic reticulum-Golgi transport vesicles. J Biol Chem. 2005 Jun 10; 280(23):22012-20. PMID: 15749703.
      View in: PubMed
    43. Zhang YW, Luo WJ, Wang H, Lin P, Vetrivel KS, Liao F, Li F, Wong PC, Farquhar MG, Thinakaran G, Xu H. Nicastrin is critical for stability and trafficking but not association of other presenilin/gamma-secretase components. J Biol Chem. 2005 Apr 29; 280(17):17020-6. PMID: 15711015; PMCID: PMC1201533.
    44. Bornheimer SJ, Maurya MR, Farquhar MG, Subramaniam S. Computational modeling reveals how interplay between components of a GTPase-cycle module regulates signal transduction. Proc Natl Acad Sci U S A. 2004 Nov 09; 101(45):15899-904. PMID: 15520372; PMCID: PMC524695.
    45. Zheng B, Lavoie C, Tang TD, Ma P, Meerloo T, Beas A, Farquhar MG. Regulation of epidermal growth factor receptor degradation by heterotrimeric Galphas protein. Mol Biol Cell. 2004 Dec; 15(12):5538-50. PMID: 15469987; PMCID: PMC532032.
    46. Schmieder S, Nagai M, Orlando RA, Takeda T, Farquhar MG. Podocalyxin activates RhoA and induces actin reorganization through NHERF1 and Ezrin in MDCK cells. J Am Soc Nephrol. 2004 Sep; 15(9):2289-98. PMID: 15339978.
      View in: PubMed
    47. Lehtonen S, Lehtonen E, Kudlicka K, Holthöfer H, Farquhar MG. Nephrin forms a complex with adherens junction proteins and CASK in podocytes and in Madin-Darby canine kidney cells expressing nephrin. Am J Pathol. 2004 Sep; 165(3):923-36. PMID: 15331416; PMCID: PMC1618613.
    48. Farquhar M. Acceptance of the 2003 A.N. Richards Award. Kidney Int. 2003 Nov; 64(5):1943-4. PMID: 14531841.
      View in: PubMed
    49. Nagai M, Meerloo T, Takeda T, Farquhar MG. The adaptor protein ARH escorts megalin to and through endosomes. Mol Biol Cell. 2003 Dec; 14(12):4984-96. PMID: 14528014; PMCID: PMC284800.
    50. Elenko E, Fischer T, Niesman I, Harding T, McQuistan T, Von Zastrow M, Farquhar MG. Spatial regulation of Galphai protein signaling in clathrin-coated membrane microdomains containing GAIP. Mol Pharmacol. 2003 Jul; 64(1):11-20. PMID: 12815156.
      View in: PubMed
    51. Fischer T, De Vries L, Meerloo T, Farquhar MG. Promotion of G alpha i3 subunit down-regulation by GIPN, a putative E3 ubiquitin ligase that interacts with RGS-GAIP. Proc Natl Acad Sci U S A. 2003 Jul 08; 100(14):8270-5. PMID: 12826607; PMCID: PMC166218.
    52. Derrien A, Zheng B, Osterhout JL, Ma YC, Milligan G, Farquhar MG, Druey KM. Src-mediated RGS16 tyrosine phosphorylation promotes RGS16 stability. J Biol Chem. 2003 May 02; 278(18):16107-16. PMID: 12588871.
      View in: PubMed
    53. Zheng B, Berrie CP, Corda D, Farquhar MG. GDE1/MIR16 is a glycerophosphoinositol phosphodiesterase regulated by stimulation of G protein-coupled receptors. Proc Natl Acad Sci U S A. 2003 Feb 18; 100(4):1745-50. PMID: 12576545; PMCID: PMC149904.
    54. Takeda T, Yamazaki H, Farquhar MG. Identification of an apical sorting determinant in the cytoplasmic tail of megalin. Am J Physiol Cell Physiol. 2003 May; 284(5):C1105-13. PMID: 12519751.
      View in: PubMed
    55. Lavoie C, Meerloo T, Lin P, Farquhar MG. Calnuc, an EF-hand Ca(2+)-binding protein, is stored and processed in the Golgi and secreted by the constitutive-like pathway in AtT20 cells. Mol Endocrinol. 2002 Nov; 16(11):2462-74. PMID: 12403836.
      View in: PubMed
    56. Lou X, McQuistan T, Orlando RA, Farquhar MG. GAIP, GIPC and Galphai3 are concentrated in endocytic compartments of proximal tubule cells: putative role in regulating megalin's function. J Am Soc Nephrol. 2002 Apr; 13(4):918-27. PMID: 11912251.
      View in: PubMed
    57. De Vries L, Farquhar MG. Screening for interacting partners for G alpha i3 and RGS-GAIP using the two-hybrid system. Methods Enzymol. 2002; 344:657-73. PMID: 11771418.
      View in: PubMed
    58. Farquhar MG, Palade GE. The Golgi apparatus: 100 years of progress and controversy. Trends Cell Biol. 1998 Jan; 8(1):2-10. PMID: 9695800.
      View in: PubMed