Huilin Zhou

InstitutionUniversity of California San Diego
DepartmentCellular and Molecular Medicine
Address9500 Gilman Drive #0660
La Jolla CA 92093
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    Gross chromosomal rearrangements (GCRs), including chromosomal translocations, inversions and other rearrangements, are a hallmark of human cancers. The goal of my laboratory is to study the specific signaling pathways, utilizing phosphorylation, sumoylation and ubiquitination that act to maintain genome integrity. We chose Saccharomyces cerevisiae as a model system due to its available genetic assays and because it allows facile biochemical and proteomic studies. Studies in my laboratory have focused on two areas: 1) the DNA damage checkpoint, and 2) protein sumoylation and ubiquitination in genome maintenance. A convergence of genetic, biochemical and proteomic approaches are being pursued to study the molecular mechanisms of signaling pathways that specifically prevent genome rearrangements.

    Research Focus Areas:

  • Biochemistry and Structural Biology

  • Cell Division and Cell Cycle Control

  • DNA Replication and Repair

  • Genetics and Genomics

  • Signal Transduction

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    Collapse Publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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    1. Liang J, Li BZ, Tan AP, Kolodner RD, Putnam CD, Zhou H. SUMO E3 ligase Mms21 prevents spontaneous DNA damage induced genome rearrangements. PLoS Genet. 2018 Mar; 14(3):e1007250. PMID: 29505562.
      View in: PubMed
    2. Gu Y, Albuquerque CP, Braas D, Zhang W, Villa GR, Bi J, Ikegami S, Masui K, Gini B, Yang H, Gahman TC, Shiau AK, Cloughesy TF, Christofk HR, Zhou H, Guan KL, Mischel PS. mTORC2 Regulates Amino Acid Metabolism in Cancer by Phosphorylation of the Cystine-Glutamate Antiporter xCT. Mol Cell. 2017 Jul 06; 67(1):128-138.e7. PMID: 28648777.
      View in: PubMed
    3. Albuquerque CP, Yeung E, Ma S, Fu T, Corbett KD, Zhou H. A Chemical and Enzymatic Approach to Study Site-Specific Sumoylation. PLoS One. 2015; 10(12):e0143810. PMID: 26633173; PMCID: PMC4669148.
    4. Pineda G, Shen Z, de Albuquerque CP, Reynoso E, Chen J, Tu CC, Tang W, Briggs S, Zhou H, Wang JY. Proteomics studies of the interactome of RNA polymerase II C-terminal repeated domain. BMC Res Notes. 2015 Oct 29; 8:616. PMID: 26515650; PMCID: PMC4627417.
    5. Lippmann QK, Diwadkar GB, Zhou H, Menefee SA. Trends in urodynamics study utilization in a Southern California managed care population. Am J Obstet Gynecol. 2015 Nov; 213(5):724.e1-6. PMID: 26164690.
      View in: PubMed
    6. Sun S, Ling SC, Qiu J, Albuquerque CP, Zhou Y, Tokunaga S, Li H, Qiu H, Bui A, Yeo GW, Huang EJ, Eggan K, Zhou H, Fu XD, Lagier-Tourenne C, Cleveland DW. ALS-causative mutations in FUS/TLS confer gain and loss of function by altered association with SMN and U1-snRNP. Nat Commun. 2015 Jan 27; 6:6171. PMID: 25625564.
      View in: PubMed
    7. Wang P, Zhou Z, Hu A, Ponte de Albuquerque C, Zhou Y, Hong L, Sierecki E, Ajiro M, Kruhlak M, Harris C, Guan KL, Zheng ZM, Newton AC, Sun P, Zhou H, Fu XD. Both decreased and increased SRPK1 levels promote cancer by interfering with PHLPP-mediated dephosphorylation of Akt. Mol Cell. 2014 May 08; 54(3):378-91. PMID: 24703948; PMCID: PMC4019712.
    8. Farber-Katz SE, Dippold HC, Buschman MD, Peterman MC, Xing M, Noakes CJ, Tat J, Ng MM, Rahajeng J, Cowan DM, Fuchs GJ, Zhou H, Field SJ. DNA damage triggers Golgi dispersal via DNA-PK and GOLPH3. Cell. 2014 Jan 30; 156(3):413-27. PMID: 24485452; PMCID: PMC4018323.
    9. Albuquerque CP, Wang G, Lee NS, Kolodner RD, Putnam CD, Zhou H. Distinct SUMO ligases cooperate with Esc2 and Slx5 to suppress duplication-mediated genome rearrangements. PLoS Genet. 2013; 9(8):e1003670. PMID: 23935535; PMCID: PMC3731205.
    10. Scafoglio C, Smolka M, Zhou H, Perissi V, Rosenfeld MG. The co-repressor SMRT delays DNA damage-induced caspase activation by repressing pro-apoptotic genes and modulating the dynamics of checkpoint kinase 2 activation. PLoS One. 2013; 8(5):e59986. PMID: 23690919; PMCID: PMC3656868.
    11. Lettman MM, Wong YL, Viscardi V, Niessen S, Chen SH, Shiau AK, Zhou H, Desai A, Oegema K. Direct binding of SAS-6 to ZYG-1 recruits SAS-6 to the mother centriole for cartwheel assembly. Dev Cell. 2013 May 13; 25(3):284-98. PMID: 23673331; PMCID: PMC3655416.
    12. Baldo V, Liang J, Wang G, Zhou H. Preserving Yeast Genetic Heritage through DNA Damage Checkpoint Regulation and Telomere Maintenance. Biomolecules. 2012 Oct 30; 2(4):505-23. PMID: 24970147; PMCID: PMC4030855.
    13. Wang G, Tong X, Weng S, Zhou H. Multiple phosphorylation of Rad9 by CDK is required for DNA damage checkpoint activation. Cell Cycle. 2012 Oct 15; 11(20):3792-800. PMID: 23070520; PMCID: PMC3495822.
    14. Fenton TR, Nathanson D, Ponte de Albuquerque C, Kuga D, Iwanami A, Dang J, Yang H, Tanaka K, Oba-Shinjo SM, Uno M, Inda MM, Wykosky J, Bachoo RM, James CD, DePinho RA, Vandenberg SR, Zhou H, Marie SK, Mischel PS, Cavenee WK, Furnari FB. Resistance to EGF receptor inhibitors in glioblastoma mediated by phosphorylation of the PTEN tumor suppressor at tyrosine 240. Proc Natl Acad Sci U S A. 2012 Aug 28; 109(35):14164-9. PMID: 22891331; PMCID: PMC3435194.
    15. Zhou H, Albuquerque CP, Liang J, Suhandynata RT, Weng S. Quantitative phosphoproteomics: New technologies and applications in the DNA damage response. Cell Cycle. 2010 Sep 01; 9(17):3479-84. PMID: 20855976; PMCID: PMC3230475.
    16. Ling SC, Albuquerque CP, Han JS, Lagier-Tourenne C, Tokunaga S, Zhou H, Cleveland DW. ALS-associated mutations in TDP-43 increase its stability and promote TDP-43 complexes with FUS/TLS. Proc Natl Acad Sci U S A. 2010 Jul 27; 107(30):13318-23. PMID: 20624952.
      View in: PubMed
    17. Chen SH, Albuquerque CP, Liang J, Suhandynata RT, Zhou H. A proteome-wide analysis of kinase-substrate network in the DNA damage response. J Biol Chem. 2010 Apr 23; 285(17):12803-12. PMID: 20190278; PMCID: PMC2857137.
    18. Dippold HC, Ng MM, Farber-Katz SE, Lee SK, Kerr ML, Peterman MC, Sim R, Wiharto PA, Galbraith KA, Madhavarapu S, Fuchs GJ, Meerloo T, Farquhar MG, Zhou H, Field SJ. GOLPH3 bridges phosphatidylinositol-4- phosphate and actomyosin to stretch and shape the Golgi to promote budding. Cell. 2009 Oct 16; 139(2):337-51. PMID: 19837035; PMCID: PMC2779841.
    19. Chen SH, Zhou H. Reconstitution of Rad53 activation by Mec1 through adaptor protein Mrc1. J Biol Chem. 2009 Jul 10; 284(28):18593-604. PMID: 19457865; PMCID: PMC2707194.
    20. Wagner MV, Smolka MB, de Bruin RA, Zhou H, Wittenberg C, Dowdy SF. Whi5 regulation by site specific CDK-phosphorylation in Saccharomyces cerevisiae. PLoS One. 2009; 4(1):e4300. PMID: 19172996; PMCID: PMC2627923.
    21. Tang T, Zheng B, Chen SH, Murphy AN, Kudlicka K, Zhou H, Farquhar MG. hNOA1 interacts with complex I and DAP3 and regulates mitochondrial respiration and apoptosis. J Biol Chem. 2009 Feb 20; 284(8):5414-24. PMID: 19103604; PMCID: PMC2643507.
    22. Payne SH, Yau M, Smolka MB, Tanner S, Zhou H, Bafna V. Phosphorylation-specific MS/MS scoring for rapid and accurate phosphoproteome analysis. J Proteome Res. 2008 Aug; 7(8):3373-81. PMID: 18563926; PMCID: PMC2566938.
    23. Lehtonen S, Shah M, Nielsen R, Iino N, Ryan JJ, Zhou H, Farquhar MG. The endocytic adaptor protein ARH associates with motor and centrosomal proteins and is involved in centrosome assembly and cytokinesis. Mol Biol Cell. 2008 Jul; 19(7):2949-61. PMID: 18417616; PMCID: PMC2441659.
    24. Albuquerque CP, Smolka MB, Payne SH, Bafna V, Eng J, Zhou H. A multidimensional chromatography technology for in-depth phosphoproteome analysis. Mol Cell Proteomics. 2008 Jul; 7(7):1389-96. PMID: 18407956; PMCID: PMC2493382.
    25. Wen G, Wessel J, Zhou W, Ehret GB, Rao F, Stridsberg M, Mahata SK, Gent PM, Das M, Cooper RS, Chakravarti A, Zhou H, Schork NJ, O'connor DT, Hamilton BA. An ancestral variant of Secretogranin II confers regulation by PHOX2 transcription factors and association with hypertension. Hum Mol Genet. 2007 Jul 15; 16(14):1752-64. PMID: 17584765; PMCID: PMC2695823.
    26. Smolka MB, Chen SH, Maddox PS, Enserink JM, Albuquerque CP, Wei XX, Desai A, Kolodner RD, Zhou H. An FHA domain-mediated protein interaction network of Rad53 reveals its role in polarized cell growth. J Cell Biol. 2006 Dec 04; 175(5):743-53. PMID: 17130285; PMCID: PMC2064674.
    27. Enserink JM, Smolka MB, Zhou H, Kolodner RD. Checkpoint proteins control morphogenetic events during DNA replication stress in Saccharomyces cerevisiae. J Cell Biol. 2006 Dec 04; 175(5):729-41. PMID: 17130284; PMCID: PMC2064673.
    28. Chen SH, Smolka MB, Zhou H. Mechanism of Dun1 activation by Rad53 phosphorylation in Saccharomyces cerevisiae. J Biol Chem. 2007 Jan 12; 282(2):986-95. PMID: 17114794; PMCID: PMC2811688.
    29. Kats ES, Albuquerque CP, Zhou H, Kolodner RD. Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants. Proc Natl Acad Sci U S A. 2006 Mar 07; 103(10):3710-5. PMID: 16501045; PMCID: PMC1533778.
    30. Velazquez-Dones A, Hagopian JC, Ma CT, Zhong XY, Zhou H, Ghosh G, Fu XD, Adams JA. Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty. J Biol Chem. 2005 Dec 16; 280(50):41761-8. PMID: 16223727.
      View in: PubMed
    31. Su YH, Chen SH, Zhou H, Vacquier VD. Tandem mass spectrometry identifies proteins phosphorylated by cyclic AMP-dependent protein kinase when sea urchin sperm undergo the acrosome reaction. Dev Biol. 2005 Sep 01; 285(1):116-25. PMID: 16038896.
      View in: PubMed
    32. Lehtonen S, Ryan JJ, Kudlicka K, Iino N, Zhou H, Farquhar MG. Cell junction-associated proteins IQGAP1, MAGI-2, CASK, spectrins, and alpha-actinin are components of the nephrin multiprotein complex. Proc Natl Acad Sci U S A. 2005 Jul 12; 102(28):9814-9. PMID: 15994232; PMCID: PMC1175008.
    33. Smolka MB, Albuquerque CP, Chen SH, Schmidt KH, Wei XX, Kolodner RD, Zhou H. Dynamic changes in protein-protein interaction and protein phosphorylation probed with amine-reactive isotope tag. Mol Cell Proteomics. 2005 Sep; 4(9):1358-69. PMID: 15972895; PMCID: PMC2813687.
    34. Audhya A, Loewith R, Parsons AB, Gao L, Tabuchi M, Zhou H, Boone C, Hall MN, Emr SD. Genome-wide lethality screen identifies new PI4,5P2 effectors that regulate the actin cytoskeleton. EMBO J. 2004 Oct 01; 23(19):3747-57. PMID: 15372071; PMCID: PMC522789.
    35. Zhou W, Ryan JJ, Zhou H. Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses. J Biol Chem. 2004 Jul 30; 279(31):32262-8. PMID: 15166219; PMCID: PMC2810850.
    36. Zhou H, Boyle R, Aebersold R. Quantitative protein analysis by solid phase isotope tagging and mass spectrometry. Methods Mol Biol. 2004; 261:511-8. PMID: 15064479.
      View in: PubMed