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    Randolph Hampton

    SchoolUniversity of California, San Diego
    DepartmentCell and Developmental Biology
    Address9500 Gilman Drive #0347
    CA La Jolla 92093
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      Collapse Research 
      Collapse Research Activities and Funding
      FASEB SRC on Ubiquitin and Cellular Regulation
      NIH/NCI R13CA168247Jul 27, 2012 - May 31, 2013
      Role: Principal Investigator
      Ubr1: A Protein Quality Control E3 Ubiquitin Ligase
      NIH/NIGMS R01GM092748Sep 30, 2011 - May 31, 2015
      Role: Principal Investigator
      Protein degradation and cholesterol regulation
      NIH/NIDDK R37DK051996Jan 1, 1997 - Jan 31, 2021
      Role: Principal Investigator
      Protein degradation and cholesterol regulation
      NIH/NIDDK R01DK051996Jan 1, 1997 - Jan 31, 2012
      Role: Principal Investigator
      IVEM and Image Analysis Resource
      NIH/NCRR P41RR004050Dec 12, 1988 - Mar 31, 2014
      Role: Co-Investigator
      Cell and Molecular Genetics Training Program
      NIH/NIGMS T32GM007240Jul 1, 1975 - Jun 30, 2020
      Role: Principal Investigator

      Collapse Bibliographic 
      Collapse Publications
      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
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      1. Hampton R, Dargemont C. New developments for protein quality control. Science. 2017 Aug 04; 357(6350):450-451. PMID: 28774913.
        View in: PubMed
      2. Neal S, Mak R, Bennett EJ, Hampton R. A Cdc48 "Retrochaperone" Function Is Required for the Solubility of Retrotranslocated, Integral Membrane Endoplasmic Reticulum-associated Degradation (ERAD-M) Substrates. J Biol Chem. 2017 Feb 24; 292(8):3112-3128. PMID: 28077573.
        View in: PubMed
      3. Akula MK, Shi M, Jiang Z, Foster CE, Miao D, Li AS, Zhang X, Gavin RM, Forde SD, Germain G, Carpenter S, Rosadini CV, Gritsman K, Chae JJ, Hampton R, Silverman N, Gravallese EM, Kagan JC, Fitzgerald KA, Kastner DL, Golenbock DT, Bergo MO, Wang D. Control of the innate immune response by the mevalonate pathway. Nat Immunol. 2016 Aug; 17(8):922-9. PMID: 27270400; PMCID: PMC4955724 [Available on 12/06/16].
      4. Vashistha N, Neal SE, Singh A, Carroll SM, Hampton R. Direct and essential function for Hrd3 in ER-associated degradation. Proc Natl Acad Sci U S A. 2016 May 24; 113(21):5934-9. PMID: 27170191; PMCID: PMC4889393 [Available on 11/24/16].
      5. Higgins R, Gendron JM, Rising L, Mak R, Webb K, Kaiser SE, Zuzow N, Riviere P, Yang B, Fenech E, Tang X, Lindsay SA, Christianson JC, Hampton R, Wasserman SA, Bennett EJ. The Unfolded Protein Response Triggers Site-Specific Regulatory Ubiquitylation of 40S Ribosomal Proteins. Mol Cell. 2015 Jul 02; 59(1):35-49. PMID: 26051182; PMCID: PMC4491043.
      6. Theesfeld CL, Hampton R. Insulin-induced gene protein (INSIG)-dependent sterol regulation of Hmg2 endoplasmic reticulum-associated degradation (ERAD) in yeast. J Biol Chem. 2013 Mar 22; 288(12):8519-30. PMID: 23306196; PMCID: PMC3605666.
      7. Hampton R, Sommer T. Finding the will and the way of ERAD substrate retrotranslocation. Curr Opin Cell Biol. 2012 Aug; 24(4):460-6. PMID: 22854296.
        View in: PubMed
      8. Li S, Spooner RA, Hampton R, Lord JM, Roberts LM. Cytosolic entry of Shiga-like toxin a chain from the yeast endoplasmic reticulum requires catalytically active Hrd1p. PLoS One. 2012; 7(7):e41119. PMID: 22829918; PMCID: PMC3400632.
      9. Theesfeld CL, Pourmand D, Davis T, Garza RM, Hampton R. The sterol-sensing domain (SSD) directly mediates signal-regulated endoplasmic reticulum-associated degradation (ERAD) of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase isozyme Hmg2. J Biol Chem. 2011 Jul 29; 286(30):26298-307. PMID: 21628456; PMCID: PMC3143592.
      10. Hampton R. San1-mediated quality control: substrate recognition "sans" chaperones. Mol Cell. 2011 Jan 07; 41(1):2-3. PMID: 21211716.
        View in: PubMed
      11. Heck JW, Cheung SK, Hampton R. Cytoplasmic protein quality control degradation mediated by parallel actions of the E3 ubiquitin ligases Ubr1 and San1. Proc Natl Acad Sci U S A. 2010 Jan 19; 107(3):1106-11. PMID: 20080635; PMCID: PMC2824284.
      12. Garza RM, Tran PN, Hampton R. Geranylgeranyl pyrophosphate is a potent regulator of HRD-dependent 3-Hydroxy-3-methylglutaryl-CoA reductase degradation in yeast. J Biol Chem. 2009 Dec 18; 284(51):35368-80. PMID: 19776008; PMCID: PMC2790966.
      13. Carroll SM, Hampton R. Usa1p is required for optimal function and regulation of the Hrd1p endoplasmic reticulum-associated degradation ubiquitin ligase. J Biol Chem. 2010 Feb 19; 285(8):5146-56. PMID: 19940128; PMCID: PMC2820741.
      14. Sato BK, Schulz D, Do PH, Hampton R. Misfolded membrane proteins are specifically recognized by the transmembrane domain of the Hrd1p ubiquitin ligase. Mol Cell. 2009 Apr 24; 34(2):212-22. PMID: 19394298; PMCID: PMC2710143.
      15. Hampton R, Garza RM. Protein quality control as a strategy for cellular regulation: lessons from ubiquitin-mediated regulation of the sterol pathway. Chem Rev. 2009 Apr; 109(4):1561-74. PMID: 19243134.
        View in: PubMed
      16. Garza RM, Sato BK, Hampton R. In vitro analysis of Hrd1p-mediated retrotranslocation of its multispanning membrane substrate 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase. J Biol Chem. 2009 May 29; 284(22):14710-22. PMID: 19324879; PMCID: PMC2685653.
      17. Hampton R. A cholesterol toggle switch. Cell Metab. 2008 Dec; 8(6):451-3. PMID: 19041760.
        View in: PubMed
      18. Federovitch CM, Jones YZ, Tong AH, Boone C, Prinz WA, Hampton R. Genetic and structural analysis of Hmg2p-induced endoplasmic reticulum remodeling in Saccharomyces cerevisiae. Mol Biol Cell. 2008 Oct; 19(10):4506-20. PMID: 18667535; PMCID: PMC2555956.
      19. Bazirgan OA, Hampton R. Cue1p is an activator of Ubc7p E2 activity in vitro and in vivo. J Biol Chem. 2008 May 09; 283(19):12797-810. PMID: 18321851; PMCID: PMC2442330.
      20. Chen D, Gould C, Garza R, Gao T, Hampton R, Newton AC. Amplitude control of protein kinase C by RINCK, a novel E3 ubiquitin ligase. J Biol Chem. 2007 Nov 16; 282(46):33776-87. PMID: 17893151.
        View in: PubMed
      21. Bazirgan OA, Garza RM, Hampton R. Determinants of RING-E2 fidelity for Hrd1p, a membrane-anchored ubiquitin ligase. J Biol Chem. 2006 Dec 22; 281(51):38989-9001. PMID: 17035235.
        View in: PubMed
      22. Sato BK, Hampton R. Yeast Derlin Dfm1 interacts with Cdc48 and functions in ER homeostasis. Yeast. 2006 Oct-Nov; 23(14-15):1053-64. PMID: 17083136.
        View in: PubMed
      23. Flury I, Garza R, Shearer A, Rosen J, Cronin S, Hampton R. INSIG: a broadly conserved transmembrane chaperone for sterol-sensing domain proteins. EMBO J. 2005 Nov 16; 24(22):3917-26. PMID: 16270032; PMCID: PMC1283954.
      24. Federovitch CM, Ron D, Hampton R. The dynamic ER: experimental approaches and current questions. Curr Opin Cell Biol. 2005 Aug; 17(4):409-14. PMID: 15975777.
        View in: PubMed
      25. Garza RM, Hampton R. Cell biology: sterol sensor comes up for air. Nature. 2005 May 05; 435(7038):37-8. PMID: 15875007.
        View in: PubMed
      26. Bazirgan OA, Hampton R. Cdc48-Ufd2-Rad23: the road less ubiquitinated? Nat Cell Biol. 2005 Mar; 7(3):207-9. PMID: 15738968.
        View in: PubMed
      27. Shearer AG, Hampton R. Lipid-mediated, reversible misfolding of a sterol-sensing domain protein. EMBO J. 2005 Jan 12; 24(1):149-59. PMID: 15635451; PMCID: PMC544911.
      28. Hampton R. Fusion-based strategies to identify genes involved in degradation of a specific substrate. Methods Enzymol. 2005; 399:310-23. PMID: 16338365.
        View in: PubMed
      29. Ron D, Hampton R. Membrane biogenesis and the unfolded protein response. J Cell Biol. 2004 Oct 11; 167(1):23-5. PMID: 15479733; PMCID: PMC2172515.
      30. Shearer AG, Hampton R. Structural control of endoplasmic reticulum-associated degradation: effect of chemical chaperones on 3-hydroxy-3-methylglutaryl-CoA reductase. J Biol Chem. 2004 Jan 02; 279(1):188-96. PMID: 14570925.
        View in: PubMed
      31. Hampton R. IRE1: a role in UPREgulation of ER degradation. Dev Cell. 2003 Feb; 4(2):144-6. PMID: 12586055.
        View in: PubMed
      32. Hampton R. ER-associated degradation in protein quality control and cellular regulation. Curr Opin Cell Biol. 2002 Aug; 14(4):476-82. PMID: 12383799.
        View in: PubMed
      33. Cronin SR, Rao R, Hampton R. Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis. J Cell Biol. 2002 Jun 10; 157(6):1017-28. PMID: 12058017; PMCID: PMC2174042.
      34. Bays NW, Hampton R. Cdc48-Ufd1-Npl4: stuck in the middle with Ub. Curr Biol. 2002 May 14; 12(10):R366-71. PMID: 12015140.
        View in: PubMed
      35. Hampton R. Proteolysis and sterol regulation. Annu Rev Cell Dev Biol. 2002; 18:345-78. PMID: 12142284.
        View in: PubMed
      36. Bays NW, Wilhovsky SK, Goradia A, Hodgkiss-Harlow K, Hampton R. HRD4/NPL4 is required for the proteasomal processing of ubiquitinated ER proteins. Mol Biol Cell. 2001 Dec; 12(12):4114-28. PMID: 11739805; PMCID: PMC60780.
      37. Gardner RG, Shearer AG, Hampton R. In vivo action of the HRD ubiquitin ligase complex: mechanisms of endoplasmic reticulum quality control and sterol regulation. Mol Cell Biol. 2001 Jul; 21(13):4276-91. PMID: 11390656; PMCID: PMC87088.
      38. Bays NW, Gardner RG, Seelig LP, Joazeiro CA, Hampton R. Hrd1p/Der3p is a membrane-anchored ubiquitin ligase required for ER-associated degradation. Nat Cell Biol. 2001 Jan; 3(1):24-9. PMID: 11146622.
        View in: PubMed
      39. Gardner RG, Shan H, Matsuda SP, Hampton R. An oxysterol-derived positive signal for 3-hydroxy- 3-methylglutaryl-CoA reductase degradation in yeast. J Biol Chem. 2001 Mar 23; 276(12):8681-94. PMID: 11134013.
        View in: PubMed
      40. Gardner RG, Swarbrick GM, Bays NW, Cronin SR, Wilhovsky S, Seelig L, Kim C, Hampton R. Endoplasmic reticulum degradation requires lumen to cytosol signaling. Transmembrane control of Hrd1p by Hrd3p. J Cell Biol. 2000 Oct 02; 151(1):69-82. PMID: 11018054; PMCID: PMC2189800.
      41. Hampton R. ER stress response: getting the UPR hand on misfolded proteins. Curr Biol. 2000 Jul 13; 10(14):R518-21. PMID: 10898996.
        View in: PubMed
      42. Wilhovsky S, Gardner R, Hampton R. HRD gene dependence of endoplasmic reticulum-associated degradation. Mol Biol Cell. 2000 May; 11(5):1697-708. PMID: 10793145; PMCID: PMC14877.
      43. Hampton R. Cholesterol homeostasis: ESCAPe from the ER. Curr Biol. 2000 Apr 20; 10(8):R298-301. PMID: 10801406.
        View in: PubMed
      44. Cronin SR, Khoury A, Ferry DK, Hampton R. Regulation of HMG-CoA reductase degradation requires the P-type ATPase Cod1p/Spf1p. J Cell Biol. 2000 Mar 06; 148(5):915-24. PMID: 10704442; PMCID: PMC2174543.
      45. Gardner RG, Hampton R. A 'distributed degron' allows regulated entry into the ER degradation pathway. EMBO J. 1999 Nov 01; 18(21):5994-6004. PMID: 10545111; PMCID: PMC1171665.
      46. Gardner RG, Hampton R. A highly conserved signal controls degradation of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase in eukaryotes. J Biol Chem. 1999 Oct 29; 274(44):31671-8. PMID: 10531376.
        View in: PubMed
      47. Cronin SR, Hampton R. Measuring protein degradation with green fluorescent protein. Methods Enzymol. 1999; 302:58-73. PMID: 12876763.
        View in: PubMed
      48. Gardner R, Cronin S, Leader B, Rine J, Hampton R, Leder B. Sequence determinants for regulated degradation of yeast 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein. Mol Biol Cell. 1998 Sep; 9(9):2611-26. PMID: 9725915; PMCID: PMC25534.
      49. Finley D, Tanaka K, Mann C, Feldmann H, Hochstrasser M, Vierstra R, Johnston S, Hampton R, Haber J, Mccusker J, Silver P, Frontali L, Thorsness P, Varshavsky A, Byers B, Madura K, Reed SI, Wolf D, Jentsch S, Sommer T, Baumeister W, Goldberg A, Fried V, Rubin DM, Toh-e A. Unified nomenclature for subunits of the Saccharomyces cerevisiae proteasome regulatory particle. Trends Biochem Sci. 1998 Jul; 23(7):244-5. PMID: 9697412.
        View in: PubMed
      50. Hampton R. Genetic analysis of hydroxymethylglutaryl-coenzyme A reductase regulated degradation. Curr Opin Lipidol. 1998 Apr; 9(2):93-7. PMID: 9559264.
        View in: PubMed
      51. Hampton R, Bhakta H. Ubiquitin-mediated regulation of 3-hydroxy-3-methylglutaryl-CoA reductase. Proc Natl Acad Sci U S A. 1997 Nov 25; 94(24):12944-8. PMID: 9371780; PMCID: PMC24243.
      52. Donald KA, Hampton R, Fritz IB. Effects of overproduction of the catalytic domain of 3-hydroxy-3-methylglutaryl coenzyme A reductase on squalene synthesis in Saccharomyces cerevisiae. Appl Environ Microbiol. 1997 Sep; 63(9):3341-4. PMID: 9292983; PMCID: PMC168639.
      53. Hampton R, Gardner RG, Rine J. Role of 26S proteasome and HRD genes in the degradation of 3-hydroxy-3-methylglutaryl-CoA reductase, an integral endoplasmic reticulum membrane protein. Mol Biol Cell. 1996 Dec; 7(12):2029-44. PMID: 8970163; PMCID: PMC276048.
      54. Hampton R, Dimster-Denk D, Rine J. The biology of HMG-CoA reductase: the pros of contra-regulation. Trends Biochem Sci. 1996 Apr; 21(4):140-5. PMID: 8701471.
        View in: PubMed
      55. Hampton R, Koning A, Wright R, Rine J. In vivo examination of membrane protein localization and degradation with green fluorescent protein. Proc Natl Acad Sci U S A. 1996 Jan 23; 93(2):828-33. PMID: 8570643; PMCID: PMC40142.
      56. Hampton R, Rine J. Regulated degradation of HMG-CoA reductase, an integral membrane protein of the endoplasmic reticulum, in yeast. J Cell Biol. 1994 Apr; 125(2):299-312. PMID: 8163547; PMCID: PMC2120026.
      57. Hampton R, Raetz CR. Lipid A 4'-kinase from Escherichia coli: enzyme assay and preparation of 4'-32P-labeled probes of high specific radioactivity. Methods Enzymol. 1992; 209:466-75. PMID: 1323052.
        View in: PubMed
      58. Hampton R, Golenbock DT, Penman M, Krieger M, Raetz CR. Recognition and plasma clearance of endotoxin by scavenger receptors. Nature. 1991 Jul 25; 352(6333):342-4. PMID: 1852209.
        View in: PubMed
      59. Hampton R, Holz RW. Effects of changes in osmolality on the stability and function of cultured chromaffin cells and the possible role of osmotic forces in exocytosis. J Cell Biol. 1983 Apr; 96(4):1082-8. PMID: 6833392; PMCID: PMC2112313.