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    Joseph Adams

    TitleProfessor
    SchoolUniversity of California, San Diego
    DepartmentPharmacology
    Address9500 Gilman Drive #0636
    CA La Jolla 92093
    Phone858-822-3360
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      Collapse Biography 
      Collapse Education and Training
      Pennsylvania State UniversityPh. D.

      Collapse Overview 
      Collapse Overview
      Our laboratory is interested in understanding how phosphorylation controls the splicing of mRNA and complexity of the human proteome. We focus attention on two families of enzymes, the SRPKs and CLKs, that phosphorylate and regulate the biological activities of SR proteins, an essential group of splicing factors that guide the assembly of the spliceosome and the selection of splice-sites in mRNA. These enzymes are not only essential for normal splicing function but are also over-expressed in many cancers making them viable targets for drug intervention in human diseases. Our laboratory applies various biochemical, biological and structural techniques to understand how these enzymes work at both the molecular and cellular levels to affect SR protein function and splicing outcomes.


      Collapse Research 
      Collapse Research Activities and Funding
      Clk Kinases and Splicing Regulation
      NIH/NIGMS R01GM098528Jun 1, 2012 - Aug 31, 2020
      Role: Principal Investigator
      Regulatory Pathways of SR Protein Kinases
      NIH/NIGMS R01GM067969Feb 1, 2004 - Jan 31, 2021
      Role: Principal Investigator
      Regulation of Nonreceptor Tyrosine Kinases through Csk.
      NIH/NIGMS R01GM068168Aug 20, 1997 - May 31, 2008
      Role: Principal Investigator
      STRUCTURAL CHARACTERIZATION OF V FPS
      NIH/NCI R01CA075112Aug 20, 1997 - Oct 31, 2003
      Role: Principal Investigator
      KINETIC BARRIERS FOR SERINE AND TYROSINE PHOSPHORYLATION
      NIH/NIGMS R29GM054846Jul 1, 1996 - Jun 30, 2002
      Role: Principal Investigator
      MECHANISTIC STUDIES OF CAMP-DEPENDENT PROTEIN KINASE
      NIH/NIGMS F32GM014528Jan 4, 1992
      Role: Principal Investigator

      Collapse ORNG Applications 
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      Collapse Bibliographic 
      Collapse Publications
      Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Researchers can login to make corrections and additions, or contact us for help.
      List All   |   Timeline
      1. Aubol BE, Hailey KL, Fattet L, Jennings PA, Adams J. Redirecting SR Protein Nuclear Trafficking through an Allosteric Platform. J Mol Biol. 2017 Jul 07; 429(14):2178-2191. PMID: 28576472.
        View in: PubMed
      2. Meharena HS, Fan X, Ahuja LG, Keshwani MM, McClendon CL, Chen AM, Adams J, Taylor SS. Decoding the Interactions Regulating the Active State Mechanics of Eukaryotic Protein Kinases. PLoS Biol. 2016 Nov; 14(11):e2000127. PMID: 27902690.
        View in: PubMed
      3. Aubol BE, Wu G, Keshwani MM, Movassat M, Fattet L, Hertel KJ, Fu XD, Adams J. Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing. Mol Cell. 2016 Jul 21; 63(2):218-228. PMID: 27397683; PMCID: PMC4941815 [Available on 07/21/17].
      4. Serrano P, Aubol BE, Keshwani MM, Forli S, Ma CT, Dutta SK, Geralt M, Wüthrich K, Adams J. Directional Phosphorylation and Nuclear Transport of the Splicing Factor SRSF1 Is Regulated by an RNA Recognition Motif. J Mol Biol. 2016 Jun 05; 428(11):2430-45. PMID: 27091468; PMCID: PMC4884534 [Available on 06/05/17].
      5. Keshwani MM, Hailey KL, Aubol BE, Fattet L, McGlone ML, Jennings PA, Adams J. Nuclear protein kinase CLK1 uses a non-traditional docking mechanism to select physiological substrates. Biochem J. 2015 Dec 15; 472(3):329-38. PMID: 26443864.
        View in: PubMed
      6. Barkho S, Pierce LC, Li S, Adams J, Jennings PA. Theoretical Insights Reveal Novel Motions in Csk's SH3 Domain That Control Kinase Activation. PLoS One. 2015; 10(6):e0127724. PMID: 26030592; PMCID: PMC4452171.
      7. Jamros MA, Aubol BE, Keshwani MM, Zhang Z, Stamm S, Adams J. Intra-domain Cross-talk Regulates Serine-arginine Protein Kinase 1-dependent Phosphorylation and Splicing Function of Transformer 2ß1. J Biol Chem. 2015 Jul 10; 290(28):17269-81. PMID: 26013829; PMCID: PMC4498066.
      8. Keshwani MM, Aubol BE, Fattet L, Ma CT, Qiu J, Jennings PA, Fu XD, Adams J. Conserved proline-directed phosphorylation regulates SR protein conformation and splicing function. Biochem J. 2015 Mar 01; 466(2):311-22. PMID: 25529026.
        View in: PubMed
      9. Aubol BE, Plocinik RM, Keshwani MM, McGlone ML, Hagopian JC, Ghosh G, Fu XD, Adams J. N-terminus of the protein kinase CLK1 induces SR protein hyperphosphorylation. Biochem J. 2014 Aug 15; 462(1):143-52. PMID: 24869919.
        View in: PubMed
      10. Aubol BE, Adams J. Recruiting a silent partner for activation of the protein kinase SRPK1. Biochemistry. 2014 Jul 22; 53(28):4625-34. PMID: 24984036; PMCID: PMC4108178.
      11. Aubol BE, Jamros MA, McGlone ML, Adams J. Splicing kinase SRPK1 conforms to the landscape of its SR protein substrate. Biochemistry. 2013 Oct 29; 52(43):7595-605. PMID: 24074032; PMCID: PMC3865722.
      12. Barkho S, Pierce LC, McGlone ML, Li S, Woods VL, Walker RC, Adams J, Jennings PA. Distal loop flexibility of a regulatory domain modulates dynamics and activity of C-terminal SRC kinase (csk). PLoS Comput Biol. 2013; 9(9):e1003188. PMID: 24039559; PMCID: PMC3764022.
      13. Aubol BE, Plocinik RM, Hagopian JC, Ma CT, McGlone ML, Bandyopadhyay R, Fu XD, Adams J. Partitioning RS domain phosphorylation in an SR protein through the CLK and SRPK protein kinases. J Mol Biol. 2013 Aug 23; 425(16):2894-909. PMID: 23707382; PMCID: PMC3860265.
      14. Zhou X, Chou TF, Aubol BE, Park CJ, Wolfenden R, Adams J, Wagner CR. Kinetic mechanism of human histidine triad nucleotide binding protein 1. Biochemistry. 2013 May 21; 52(20):3588-600. PMID: 23614568; PMCID: PMC3835729.
      15. Jamros MA, Oliveira LC, Whitford PC, Onuchic JN, Adams J, Jennings PA. Substrate-specific reorganization of the conformational ensemble of CSK implicates novel modes of kinase function. PLoS Comput Biol. 2012; 8(9):e1002695. PMID: 23028292; PMCID: PMC3447962.
      16. Aubol BE, Plocinik RM, McGlone ML, Adams J. Nucleotide release sequences in the protein kinase SRPK1 accelerate substrate phosphorylation. Biochemistry. 2012 Aug 21; 51(33):6584-94. PMID: 22839969; PMCID: PMC3718016.
      17. Zhou Z, Qiu J, Liu W, Zhou Y, Plocinik RM, Li H, Hu Q, Ghosh G, Adams J, Rosenfeld MG, Fu XD. The Akt-SRPK-SR axis constitutes a major pathway in transducing EGF signaling to regulate alternative splicing in the nucleus. Mol Cell. 2012 Aug 10; 47(3):422-33. PMID: 22727668; PMCID: PMC3418396.
      18. Steichen JM, Kuchinskas M, Keshwani MM, Yang J, Adams J, Taylor SS. Structural basis for the regulation of protein kinase A by activation loop phosphorylation. J Biol Chem. 2012 Apr 27; 287(18):14672-80. PMID: 22334660; PMCID: PMC3340281.
      19. Sumanasekera C, Kelemen O, Beullens M, Aubol BE, Adams J, Sunkara M, Morris A, Bollen M, Andreadis A, Stamm S. C6 pyridinium ceramide influences alternative pre-mRNA splicing by inhibiting protein phosphatase-1. Nucleic Acids Res. 2012 May; 40(9):4025-39. PMID: 22210893; PMCID: PMC3351148.
      20. Aubol BE, Adams J. Applying the brakes to multisite SR protein phosphorylation: substrate-induced effects on the splicing kinase SRPK1. Biochemistry. 2011 Aug 16; 50(32):6888-900. PMID: 21728354; PMCID: PMC3153611.
      21. Plocinik RM, Li S, Liu T, Hailey KL, Whitesides J, Whitehouse J, Ma CT, Fu XD, Gosh G, Woods VL, Jennings PA, Adams J. Regulating SR protein phosphorylation through regions outside the kinase domain of SRPK1. J Mol Biol. 2011 Jul 01; 410(1):131-45. PMID: 21600902; PMCID: PMC3121894.
      22. Ghosh G, Adams J. Phosphorylation mechanism and structure of serine-arginine protein kinases. FEBS J. 2011 Feb; 278(4):587-97. PMID: 21205204; PMCID: PMC3079193.
      23. Ma CT, Ghosh G, Fu XD, Adams J. Mechanism of dephosphorylation of the SR protein ASF/SF2 by protein phosphatase 1. J Mol Biol. 2010 Oct 29; 403(3):386-404. PMID: 20826166; PMCID: PMC2994579.
      24. Jamros MA, Oliveira LC, Whitford PC, Onuchic JN, Adams J, Blumenthal DK, Jennings PA. Proteins at work: a combined small angle X-RAY scattering and theoretical determination of the multiple structures involved on the protein kinase functional landscape. J Biol Chem. 2010 Nov 12; 285(46):36121-8. PMID: 20801888; PMCID: PMC2975234.
      25. Plesniak LA, Salzameda B, Hinderberger H, Regan E, Kahn J, Mills SA, Teriete P, Yao Y, Jennings P, Marassi F, Adams J. Structure and activity of CPNGRC: a modified CD13/APN peptidic homing motif. Chem Biol Drug Des. 2010 Jun; 75(6):551-62. PMID: 20374250; PMCID: PMC2890305.
      26. Huynh N, Ma CT, Giang N, Hagopian J, Ngo J, Adams J, Ghosh G. Allosteric interactions direct binding and phosphorylation of ASF/SF2 by SRPK1. Biochemistry. 2009 Dec 08; 48(48):11432-40. PMID: 19886675; PMCID: PMC3322178.
      27. Ma CT, Hagopian JC, Ghosh G, Fu XD, Adams J. Regiospecific phosphorylation control of the SR protein ASF/SF2 by SRPK1. J Mol Biol. 2009 Jul 24; 390(4):618-34. PMID: 19477182; PMCID: PMC2741142.
      28. Zhong XY, Ding JH, Adams J, Ghosh G, Fu XD. Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones. Genes Dev. 2009 Feb 15; 23(4):482-95. PMID: 19240134; PMCID: PMC2648651.
      29. Hyeon C, Jennings PA, Adams J, Onuchic JN. Ligand-induced global transitions in the catalytic domain of protein kinase A. Proc Natl Acad Sci U S A. 2009 Mar 03; 106(9):3023-8. PMID: 19204278; PMCID: PMC2651249.
      30. Hagopian JC, Ma CT, Meade BR, Albuquerque CP, Ngo JC, Ghosh G, Jennings PA, Fu XD, Adams J. Adaptable molecular interactions guide phosphorylation of the SR protein ASF/SF2 by SRPK1. J Mol Biol. 2008 Oct 17; 382(4):894-909. PMID: 18687337; PMCID: PMC2741138.
      31. Ngo JC, Giang K, Chakrabarti S, Ma CT, Huynh N, Hagopian JC, Dorrestein PC, Fu XD, Adams J, Ghosh G. A sliding docking interaction is essential for sequential and processive phosphorylation of an SR protein by SRPK1. Mol Cell. 2008 Mar 14; 29(5):563-76. PMID: 18342604; PMCID: PMC2852395.
      32. Plesniak LA, Botsch K, Leibrand M, Kelly M, Sem D, Adams J, Jennings P. Transferred NOE and saturation transfer difference NMR studies of novobiocin binding to EnvZ suggest binding mode similar to DNA gyrase. Chem Biol Drug Des. 2008 Jan; 71(1):28-35. PMID: 18093089.
        View in: PubMed
      33. Valiev M, Yang J, Adams J, Taylor SS, Weare JH. Phosphorylation reaction in cAPK protein kinase-free energy quantum mechanical/molecular mechanics simulations. J Phys Chem B. 2007 Nov 29; 111(47):13455-64. PMID: 17983217.
        View in: PubMed
      34. Ma CT, Velazquez-Dones A, Hagopian JC, Ghosh G, Fu XD, Adams J. Ordered multi-site phosphorylation of the splicing factor ASF/SF2 by SRPK1. J Mol Biol. 2008 Feb 08; 376(1):55-68. PMID: 18155240.
        View in: PubMed
      35. Lukasiewicz R, Velazquez-Dones A, Huynh N, Hagopian J, Fu XD, Adams J, Ghosh G. Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions. J Biol Chem. 2007 Aug 10; 282(32):23036-43. PMID: 17517895.
        View in: PubMed
      36. Ngo JC, Gullingsrud J, Giang K, Yeh MJ, Fu XD, Adams J, McCammon JA, Ghosh G. SR protein kinase 1 is resilient to inactivation. Structure. 2007 Jan; 15(1):123-33. PMID: 17223538.
        View in: PubMed
      37. Lukasiewicz R, Nolen B, Adams J, Ghosh G. The RGG domain of Npl3p recruits Sky1p through docking interactions. J Mol Biol. 2007 Mar 16; 367(1):249-61. PMID: 17239901.
        View in: PubMed
      38. Mills JE, Whitford PC, Shaffer J, Onuchic JN, Adams J, Jennings PA. A novel disulfide bond in the SH2 Domain of the C-terminal Src kinase controls catalytic activity. J Mol Biol. 2007 Feb 02; 365(5):1460-8. PMID: 17137590; PMCID: PMC2741090.
      39. Lieser SA, Shaffer J, Adams J. SRC tail phosphorylation is limited by structural changes in the regulatory tyrosine kinase Csk. J Biol Chem. 2006 Dec 08; 281(49):38004-12. PMID: 17018524.
        View in: PubMed
      40. Ding JH, Zhong XY, Hagopian JC, Cruz MM, Ghosh G, Feramisco J, Adams J, Fu XD. Regulated cellular partitioning of SR protein-specific kinases in mammalian cells. Mol Biol Cell. 2006 Feb; 17(2):876-85. PMID: 16319169; PMCID: PMC1356596.
      41. Velazquez-Dones A, Hagopian JC, Ma CT, Zhong XY, Zhou H, Ghosh G, Fu XD, Adams J. Mass spectrometric and kinetic analysis of ASF/SF2 phosphorylation by SRPK1 and Clk/Sty. J Biol Chem. 2005 Dec 16; 280(50):41761-8. PMID: 16223727.
        View in: PubMed
      42. Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams J, Fu XD, Ghosh G. Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2. Mol Cell. 2005 Oct 07; 20(1):77-89. PMID: 16209947.
        View in: PubMed
      43. Lieser SA, Aubol BE, Wong L, Jennings PA, Adams J. Coupling phosphoryl transfer and substrate interactions in protein kinases. Biochim Biophys Acta. 2005 Dec 30; 1754(1-2):191-9. PMID: 16213199.
        View in: PubMed
      44. Wong L, Lieser SA, Miyashita O, Miller M, Tasken K, Onuchic JN, Adams J, Woods VL, Jennings PA. Coupled motions in the SH2 and kinase domains of Csk control Src phosphorylation. J Mol Biol. 2005 Aug 05; 351(1):131-43. PMID: 16002086.
        View in: PubMed
      45. Lieser SA, Shindler C, Aubol BE, Lee S, Sun G, Adams J. Phosphoryl transfer step in the C-terminal Src kinase controls Src recognition. J Biol Chem. 2005 Mar 04; 280(9):7769-76. PMID: 15623523.
        View in: PubMed
      46. Wong L, Lieser S, Chie-Leon B, Miyashita O, Aubol B, Shaffer J, Onuchic JN, Jennings PA, Woods VL, Adams J. Dynamic coupling between the SH2 domain and active site of the COOH terminal Src kinase, Csk. J Mol Biol. 2004 Jul 30; 341(1):93-106. PMID: 15312765.
        View in: PubMed
      47. Aubol BE, Ungs L, Lukasiewicz R, Ghosh G, Adams J. Chemical clamping allows for efficient phosphorylation of the RNA carrier protein Npl3. J Biol Chem. 2004 Jul 16; 279(29):30182-8. PMID: 15145958.
        View in: PubMed
      48. Wong L, Jennings PA, Adams J. Communication pathways between the nucleotide pocket and distal regulatory sites in protein kinases. Acc Chem Res. 2004 May; 37(5):304-11. PMID: 15147171.
        View in: PubMed
      49. Aubol BE, Nolen B, Shaffer J, Ghosh G, Adams J. Novel destabilization of nucleotide binding by the gamma phosphate of ATP in the yeast SR protein kinase Sky1p. Biochemistry. 2003 Nov 11; 42(44):12813-20. PMID: 14596595.
        View in: PubMed
      50. Aubol BE, Chakrabarti S, Ngo J, Shaffer J, Nolen B, Fu XD, Ghosh G, Adams J. Processive phosphorylation of alternative splicing factor/splicing factor 2. Proc Natl Acad Sci U S A. 2003 Oct 28; 100(22):12601-6. PMID: 14555757; PMCID: PMC240664.
      51. Valiev M, Kawai R, Adams J, Weare JH. The role of the putative catalytic base in the phosphoryl transfer reaction in a protein kinase: first-principles calculations. J Am Chem Soc. 2003 Aug 20; 125(33):9926-7. PMID: 12914447.
        View in: PubMed
      52. Nolen B, Ngo J, Chakrabarti S, Vu D, Adams J, Ghosh G. Nucleotide-induced conformational changes in the Saccharomyces cerevisiae SR protein kinase, Sky1p, revealed by X-ray crystallography. Biochemistry. 2003 Aug 19; 42(32):9575-85. PMID: 12911299.
        View in: PubMed
      53. Adams J. Activation loop phosphorylation and catalysis in protein kinases: is there functional evidence for the autoinhibitor model? Biochemistry. 2003 Jan 28; 42(3):601-7. PMID: 12534271.
        View in: PubMed
      54. Moore MJ, Adams J, Taylor SS. Structural basis for peptide binding in protein kinase A. Role of glutamic acid 203 and tyrosine 204 in the peptide-positioning loop. J Biol Chem. 2003 Mar 21; 278(12):10613-8. PMID: 12499371.
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      55. Plesniak L, Horiuchi Y, Sem D, Meinenger D, Stiles L, Shaffer J, Jennings PA, Adams J. Probing the nucleotide binding domain of the osmoregulator EnvZ using fluorescent nucleotide derivatives. Biochemistry. 2002 Nov 26; 41(47):13876-82. PMID: 12437344.
        View in: PubMed
      56. Hamuro Y, Wong L, Shaffer J, Kim JS, Stranz DD, Jennings PA, Woods VL, Adams J. Phosphorylation driven motions in the COOH-terminal Src kinase, CSK, revealed through enhanced hydrogen-deuterium exchange and mass spectrometry (DXMS). J Mol Biol. 2002 Nov 08; 323(5):871-81. PMID: 12417200.
        View in: PubMed
      57. Aubol BE, Nolen B, Vu D, Ghosh G, Adams J. Mechanistic insights into Sky1p, a yeast homologue of the mammalian SR protein kinases. Biochemistry. 2002 Aug 06; 41(31):10002-9. PMID: 12146964.
        View in: PubMed
      58. Shaffer J, Sun G, Adams J. Nucleotide release and associated conformational changes regulate function in the COOH-terminal Src kinase, Csk. Biochemistry. 2001 Sep 18; 40(37):11149-55. PMID: 11551213.
        View in: PubMed
      59. Leon BC, Tsigelny I, Adams J. Electrostatic environment surrounding the activation loop phosphotyrosine in the oncoprotein v-Fps. Biochemistry. 2001 Aug 28; 40(34):10078-86. PMID: 11513586.
        View in: PubMed
      60. Adams J. Kinetic and catalytic mechanisms of protein kinases. Chem Rev. 2001 Aug; 101(8):2271-90. PMID: 11749373.
        View in: PubMed
      61. Andersen MD, Shaffer J, Jennings PA, Adams J. Structural characterization of protein kinase A as a function of nucleotide binding. Hydrogen-deuterium exchange studies using matrix-assisted laser desorption ionization-time of flight mass spectrometry detection. J Biol Chem. 2001 Apr 27; 276(17):14204-11. PMID: 11278927.
        View in: PubMed
      62. Hirai TJ, Tsigelny I, Adams J. Catalytic assessment of the glycine-rich loop of the v-Fps oncoprotein using site-directed mutagenesis. Biochemistry. 2000 Oct 31; 39(43):13276-84. PMID: 11052681.
        View in: PubMed
      63. Ni Q, Shaffer J, Adams J. Insights into nucleotide binding in protein kinase A using fluorescent adenosine derivatives. Protein Sci. 2000 Sep; 9(9):1818-27. PMID: 11045627; PMCID: PMC2144695.
      64. Konkol L, Hirai TJ, Adams J. Substrate specificity of the oncoprotein v-Fps: site-specific mutagenesis of the putative P+1 pocket. Biochemistry. 2000 Jan 11; 39(1):255-62. PMID: 10625501.
        View in: PubMed
      65. Shaffer J, Adams J. Detection of conformational changes along the kinetic pathway of protein kinase A using a catalytic trapping technique. Biochemistry. 1999 Sep 14; 38(37):12072-9. PMID: 10508411.
        View in: PubMed
      66. Shaffer J, Adams J. An ATP-linked structural change in protein kinase A precedes phosphoryl transfer under physiological magnesium concentrations. Biochemistry. 1999 Apr 27; 38(17):5572-81. PMID: 10220345.
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      67. Saylor P, Hanna E, Adams J. Mutations in the activation loop tyrosine of the oncoprotein v-Fps. Biochemistry. 1998 Dec 22; 37(51):17875-81. PMID: 9922154.
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      68. Saylor P, Wang C, Hirai TJ, Adams J. A second magnesium ion is critical for ATP binding in the kinase domain of the oncoprotein v-Fps. Biochemistry. 1998 Sep 08; 37(36):12624-30. PMID: 9730835.
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      69. Grant BD, Hemmer W, Tsigelny I, Adams J, Taylor SS. Kinetic analyses of mutations in the glycine-rich loop of cAMP-dependent protein kinase. Biochemistry. 1998 May 26; 37(21):7708-15. PMID: 9601030.
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      70. Zhou J, Adams J. Participation of ADP dissociation in the rate-determining step in cAMP-dependent protein kinase. Biochemistry. 1997 Dec 16; 36(50):15733-8. PMID: 9398302.
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      71. Miyawaki A, Llopis J, Heim R, McCaffery JM, Adams J, Ikura M, Tsien RY. Fluorescent indicators for Ca2+ based on green fluorescent proteins and calmodulin. Nature. 1997 Aug 28; 388(6645):882-7. PMID: 9278050.
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      72. Lew J, Taylor SS, Adams J. Identification of a partially rate-determining step in the catalytic mechanism of cAMP-dependent protein kinase: a transient kinetic study using stopped-flow fluorescence spectroscopy. Biochemistry. 1997 Jun 03; 36(22):6717-24. PMID: 9184152.
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      73. Zhou J, Adams J. Is there a catalytic base in the active site of cAMP-dependent protein kinase? Biochemistry. 1997 Mar 11; 36(10):2977-84. PMID: 9062128.
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      74. Adams J. Insight into tyrosine phosphorylation in v-Fps using proton inventory techniques. Biochemistry. 1996 Aug 20; 35(33):10949-56. PMID: 8718888.
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      75. Grant BD, Tsigelny I, Adams J, Taylor SS. Examination of an active-site electrostatic node in the cAMP-dependent protein kinase catalytic subunit. Protein Sci. 1996 Jul; 5(7):1316-24. PMID: 8819164; PMCID: PMC2143462.
      76. Evenson DA, Adams J, McIvor RS, Wagner CR. Methotrexate resistance of mouse dihydrofolate reductase: effect of substitution of phenylalanine-31 by serine or tryptophan. J Med Chem. 1996 Apr 26; 39(9):1763-6. PMID: 8627598.
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      77. Grant BD, Adams J. Pre-steady-state kinetic analysis of cAMP-dependent protein kinase using rapid quench flow techniques. Biochemistry. 1996 Feb 13; 35(6):2022-9. PMID: 8639687.
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      78. Wang C, Lee TR, Lawrence DS, Adams J. Rate-determining steps for tyrosine phosphorylation by the kinase domain of v-fps. Biochemistry. 1996 Feb 06; 35(5):1533-9. PMID: 8634284.
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      79. Gish G, McGlone ML, Pawson T, Adams J. Bacterial expression, purification and preliminary kinetic description of the kinase domain of v-fps. Protein Eng. 1995 Jun; 8(6):609-14. PMID: 8532686.
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